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1e4o

Phosphorylase recognition and phosphorolysis of its oligosaccharide substrate: answers to a long outstanding question

Method: X-RAY DIFFRACTION D_max: 127.9 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1e4o
deposition_date	2000-07-11
title	Phosphorylase recognition and phosphorolysis of its oligosaccharide substrate: answers to a long outstanding question
keywords	MALTOPENTAOSE, CARBOHYDRATE RECOGNITION, PHOSPHORYLASE, HYDROLASE, BINARY AND TERNARY OLIGOSACCHARIDE COMPLEXES; HYDROLASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	38.73 Å
rg_electron	38.56 Å
i0	489787000.00
molecular_weight	182640.0 kDa
excluded_volume	229120 Å³
envelope_volume	276080 Å³
shell_volume	59008 Å³
envelope_diameter	132.1 Å
shell_rg	44.82 Å
envelope_rg	38.46 Å
shape_rg	38.54 Å
total_rg	38.97 Å
total_atoms	12898
n_residues	1592
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	127.9 Å
rg_real	38.78 Å
rg_real_error	1.05 Å
i0_real	4.8980e+08
i0_real_error	8.2050e+06
rg_reciprocal	38.75 Å
i0_reciprocal	489800000.0000
total_estimate	0.8835
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	40.6 Å
skewness	0.358
kurtosis	-0.478
angular_range	— – 0.2050 Å⁻¹
current_alpha	0.0000
highest_alpha	184700000.0000
n_real_points	42
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.878; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.992; Smooth: 0.856

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	MALTODEXTRIN PHOSPHORYLASE
formula_weight	90497.0 kDa
ec_number	2.4.1.1
src_method	nat
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	13251

SQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALAEMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDLLEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPDDWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPLRLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQCACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAWAITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEKVWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTNGITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRDQYREIKQANKVRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVPRVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIPAADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVEQVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYLVMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR

Entity 2 branched

description	;alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose ;
formula_weight	828.7 kDa
src_method	man
entity_count	2

Entity 3 non-polymer

description	PYRIDOXAL-5'-PHOSPHATE
formula_weight	247.1 kDa
src_method	syn
entity_count	2

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	353

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6. Citations (1)

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length_a	74.450 Å
length_b	105.690 Å
length_c	214.910 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	2.900 Å
resolution_low	50.000 Å
r_factor	0.2450
r_work	0.2450
r_free	0.3060

fit	/app/data/saxs_computation/e4/1e4o/1e4o.fit
pddf	/app/data/pr_computation/e4/1e4o/1e4o.pr.out
profile	/app/data/saxs_computation/e4/1e4o/1e4o.dat

Phosphorylase recognition and phosphorolysis of its oligosaccharide substrate: answers to a long outstanding question

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction