← Back to search

1e5n

E246C mutant of P fluorescens subsp. cellulosa xylanase A in complex with xylopentaose

Method: X-RAY DIFFRACTION D_max: 79.2 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	1e5n
deposition_date	2000-07-27
title	E246C mutant of P fluorescens subsp. cellulosa xylanase A in complex with xylopentaose
keywords	GLYCOSYL HYDROLASE, FAMILY 10, XYLAN DEGRADATION, HYDROLASE; HYDROLASE
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	26.62 Å
rg_electron	25.73 Å
i0	104958000.00
molecular_weight	77880.0 kDa
excluded_volume	96147 Å³
envelope_volume	112780 Å³
shell_volume	35548 Å³
envelope_diameter	80.1 Å
shell_rg	34.12 Å
envelope_rg	25.45 Å
shape_rg	25.72 Å
total_rg	26.58 Å
total_atoms	5494
n_residues	692
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	79.2 Å
rg_real	26.48 Å
rg_real_error	0.39 Å
i0_real	1.0500e+08
i0_real_error	1.3270e+06
rg_reciprocal	26.53 Å
i0_reciprocal	105000000.0000
total_estimate	0.9085
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	1
r_peak_primary	77.9 Å
skewness	0.198
kurtosis	-0.494
angular_range	— – 0.3000 Å⁻¹
current_alpha	0.0000
highest_alpha	41510000.0000
n_real_points	61
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.956; Stabil: 0.998; Sysdev: 1.000; Positv: 1.000; Valcen: 0.990; Smooth: 0.955

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (3)

Entity 1 polymer

description	ENDO-1,4-BETA-XYLANASE A
formula_weight	38548.6 kDa
ec_number	3.2.1.8
mutation	YES
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	5494

NGLASLADFPIGVAVAASGGNADIFTSSARQNIVRAEFNQITAENIMKMSYMYSGSNFSFTNSDRLVSWAAQNGQTVHGHALVWHPSYQLPNWASDSNANFRQDFARHIDTVAAHFAGQVKSWDVVNEALFDSADDPDGRGSANGYRQSVFYRQFGGPEYIDEAFRRARAADPTAELYYNDFNTEENGAKTTALVNLVQRLLNNGVPIDGVGFQMHVMNDYPSIANIRQAMQKIVALSPTLKIKITCLDVRLNNPYDGNSSNDYTNRNDCAVSCAGLDRQKARYKEIVQAYLEVVPPGRRGGITVWGIADPDSWLYTHQNLPDWPLLFNDNLQPKPAYQGVVEALSGR

Source

organism	PSEUDOMONAS FLUORESCENS
ncbi_taxonomy_id	294
host_organism	ESCHERICHIA COLI

Entity 2 branched

description	beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose
formula_weight	678.6 kDa
src_method	man
entity_count	2

Entity 3 non-polymer

description	CALCIUM ION
formula_weight	40.1 kDa
src_method	syn
entity_count	2

▶

6. Citations (4)

▶

length_a	96.700 Å
length_b	96.700 Å
length_c	152.700 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	16

space_group	P 43 21 2
int_tables_number	96
crystal_system	—

resolution	3.200 Å
resolution_low	29.700 Å
r_factor	0.1900
r_work	0.1900
r_free	0.2450

fit	/app/data/saxs_computation/e5/1e5n/1e5n.fit
pddf	/app/data/pr_computation/e5/1e5n/1e5n.pr.out
profile	/app/data/saxs_computation/e5/1e5n/1e5n.dat

E246C mutant of P fluorescens subsp. cellulosa xylanase A in complex with xylopentaose

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction