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1e5t

PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN, MUTANT

Method: X-RAY DIFFRACTION D_max: 81.6 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1e5t
deposition_date	2000-08-02
title	PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN, MUTANT
keywords	HYDROLASE, PROLYL OLIGOPEPTIDASE, AMNESIA, ALPHA/ BETA-HYDROLASE, BETA-PROPELLER; HYDROLASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	26.99 Å
rg_electron	25.85 Å
i0	104977000.00
molecular_weight	81551.0 kDa
excluded_volume	102330 Å³
envelope_volume	122710 Å³
shell_volume	38173 Å³
envelope_diameter	85.2 Å
shell_rg	34.61 Å
envelope_rg	25.68 Å
shape_rg	25.83 Å
total_rg	26.80 Å
total_atoms	5752
n_residues	710
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	81.6 Å
rg_real	26.81 Å
rg_real_error	0.41 Å
i0_real	1.0500e+08
i0_real_error	1.3830e+06
rg_reciprocal	26.86 Å
i0_reciprocal	105000000.0000
total_estimate	0.9030
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	34.6 Å
skewness	0.149
kurtosis	-0.421
angular_range	— – 0.2950 Å⁻¹
current_alpha	0.0000
highest_alpha	20450000.0000
n_real_points	60
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.927; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.984; Smooth: 0.969

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	PROLYL ENDOPEPTIDASE
formula_weight	80839.3 kDa
ec_number	3.4.21.26
mutation	YES
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	6618

MLSFQYPDVYRDETAIQDYHGHKVCDPYAWLEDPDSEQTKAFVEAQNKITVPFLEQCPIRGLYKERMTELYDYPKYSCHFKKGKRYFYFYNTGLQNQRVLYVQDSLEGEARVFLDPNILSDDGTVALRGYAFSEDGEYFAYGLSASGSDWVTIKFMKVDGAKELPDVLERVKFSCMAWTHDGKGMFYNAYPQQDGKSDGTETSTNLHQKLYYHVLGTDQSEDILCAEFPDEPKWMGGAELSDDGRYVLLSIREGTDPVNRLWYCDLQQESNGITGILKWVKLIDNFEGEYDYVTNEGTVFTFKTNRHSPNYRLINIDFTDPEESKWKVLVPEHEKDVLEWVACVRSNFLVLCYLHDVKNTLQLHDLATGALLKIFPLEVGSVVGYSGQKKDTEIFYCFTSFLSPGIIYHCDLTKEELEPRVFREVTVKGIDASDYQTVQIFYPSKDGTKIPMFIVHKKGIKLDGSHPAFLYGYGGFNISITPNYSVSRLIFVRHMGGVLAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAEYLIKEGYTSPKRLTINGGSNGGLLVATCANQRPDLFGCVIAQVGVMDMLKFHKYTIGHAWTTDYGCSDSKQHFEWLIKYSPLHNVKLPEADDIQYPSMLLLTADHDDRVVPLHSLKFIATLQYIVGRSRKQNNPLLIHVDTKAGHGAGMPTAKVIEEVSDMFAFIARCLNIDWIP

Source

organism	SUS SCROFA
common_name	PIG
ncbi_taxonomy_id	9823
host_organism	ESCHERICHIA COLI

Entity 2 non-polymer

description	GLYCEROL
formula_weight	92.1 kDa
src_method	syn
entity_count	9

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	866

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6. Citations (2)

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length_a	70.700 Å
length_b	99.700 Å
length_c	110.900 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	1.700 Å
resolution_low	21.000 Å
r_factor	0.1840
r_work	0.1840
r_free	0.2060

fit	/app/data/saxs_computation/e5/1e5t/1e5t.fit
pddf	/app/data/pr_computation/e5/1e5t/1e5t.pr.out
profile	/app/data/saxs_computation/e5/1e5t/1e5t.dat

PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN, MUTANT

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction