1ea4

TRANSCRIPTIONAL REPRESSOR COPG/22bp dsDNA COMPLEX

Method: X-RAY DIFFRACTION

▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

TRANSCRIPTIONAL REPRESSOR COPG

STREPTOCOCCUS AGALACTIAE

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–DNA	Homooligomer	Protein 20 DNA 12	;DNA (5'-D(TPAPAPCPCPGPTPGP CPAPCPTPCPAPAPTPGPCPAPAPTPC)-3') ; × 6 ;DNA(5'-D(APGPAPTPTPGPCPAPTP TPGPAPGPTPGPCPAPCPGPGPTPT)-3') ; × 6 water × 32	Consistent with all polymers
2	Protein–DNA	Homooligomer	Protein 4 DNA 2	;DNA (5'-D(TPAPAPCPCPGPTPGP CPAPCPTPCPAPAPTPGPCPAPAPTPC)-3') ; × 1 ;DNA(5'-D(APGPAPTPTPGPCPAPTP TPGPAPGPTPGPCPAPCPGPGPTPT)-3') ; × 1 water × 6	Consistent with all polymers
3	Protein–DNA	Homooligomer	Protein 4 DNA 2	;DNA (5'-D(TPAPAPCPCPGPTPGP CPAPCPTPCPAPAPTPGPCPAPAPTPC)-3') ; × 1 ;DNA(5'-D(APGPAPTPTPGPCPAPTP TPGPAPGPTPGPCPAPCPGPGPTPT)-3') ; × 1 water × 6	Consistent with all polymers
4	Protein–DNA	Homooligomer	Protein 4 DNA 4	;DNA (5'-D(TPAPAPCPCPGPTPGP CPAPCPTPCPAPAPTPGPCPAPAPTPC)-3') ; × 2 ;DNA(5'-D(APGPAPTPTPGPCPAPTP TPGPAPGPTPGPCPAPCPGPGPTPT)-3') ; × 2 water × 8	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	REPA_STRPN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–45； UniProt 1–45 Author chain B； PDBConstruct 1–45； UniProt 1–45 Author chain D； PDBConstruct 1–45； UniProt 1–45 Author chain E； PDBConstruct 1–45； UniProt 1–45 Author chain F； PDBConstruct 1–45； UniProt 1–45 Author chain G； PDBConstruct 1–45； UniProt 1–45 Author chain H； PDBConstruct 1–45； UniProt 1–45 Author chain J； PDBConstruct 1–45； UniProt 1–45 Author chain K； PDBConstruct 1–45； UniProt 1–45 Author chain L； PDBConstruct 1–45； UniProt 1–45

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id	1ea4
Deposition date deposition_date	2000-11-05
Structure title title	TRANSCRIPTIONAL REPRESSOR COPG/22bp dsDNA COMPLEX
Keywords keywords	TRANSCRIPTIONAL REPRESSOR, DNA-BINDING PROTEIN, PLASMID, PROTEIN-DNA COMPLEX, GENE REGULATION/DNA, GENE REGULATION-DNA complex; GENE REGULATION/DNA
Experimental Method method	X-RAY DIFFRACTION

▼

3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1ea4__assembly_3__model_1

Assembly 3 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1ea4__assembly_3__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

1ea4__assembly_3__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	21.30 Å
Rg (electron density)	19.98 Å
Total Rg	20.77 Å
Atom count	2174
Residues	216
Excluded volume	36625 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`1ea4__assembly_1__model_1`	32-meric （32）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`1ea4__assembly_2__model_1`	hexameric （6）	Success	4.1.3-1-20251215 (887e7ef)	View Download
3	1	`1ea4__assembly_3__model_1`	hexameric （6）	Success	4.1.3-1-20251215 (887e7ef)	View Download
4	1	`1ea4__assembly_4__model_1`	octameric （8）	Success	4.1.3-1-20251215 (887e7ef)	View Download

▶

4. Crystallography and Experiment 4. Crystallography & Experiment

▶

5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Description description	TRANSCRIPTIONAL REPRESSOR COPG
Molecular weight formula_weight	5124.1 kDa
Source method src_method	man
Copy count entity_count	10

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,D,E,F,G,H,J,K,L
Atom count atom_count	6013

MKKRLTITLSESVLENLEKMAREMGLSKSAMISVALENYKKGQEK

Source Source

Source organism organism	STREPTOCOCCUS AGALACTIAE
NCBI taxonomy ID ncbi_taxonomy_id	1311
Host organism host_organism	ESCHERICHIA COLI

Entity 2 Entity 2 polymer

Description description	;DNA (5'-D(TPAPAPCPCPGPTPGP CPAPCPTPCPAPAPTPGPCPAPAPTPC)-3') ;
Molecular weight formula_weight	6680.3 kDa
Source method src_method	syn
Copy count entity_count	3

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	U,W,Y

TAACCGTGCACTCAATGCAATC

Entity 3 Entity 3 polymer

Description description	;DNA(5'-D(APGPAPTPTPGPCPAPTP TPGPAPGPTPGPCPAPCPGPGPTPT)-3') ;
Molecular weight formula_weight	6822.4 kDa
Source method src_method	syn
Copy count entity_count	3

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	V,X,Z

AGATTGCATTGAGTGCACGGTT

Entity 4 Entity 4 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	101

▼

6. Fold Classification (SCOP + CATH) 20 domains

SCOP 2.08 (10 domains)

Domain ID domain_id	d1ea4a_
Class class	a — All alpha proteins
Fold Fold fold	a.43 — Ribbon-helix-helix
Superfamily Superfamily superfamily	a.43.1 — Ribbon-helix-helix
Family Family family	a.43.1.3 — CopG-like

Domain ID domain_id	d1ea4b_
Class class	a — All alpha proteins
Fold Fold fold	a.43 — Ribbon-helix-helix
Superfamily Superfamily superfamily	a.43.1 — Ribbon-helix-helix
Family Family family	a.43.1.3 — CopG-like

Domain ID domain_id	d1ea4d_
Class class	a — All alpha proteins
Fold Fold fold	a.43 — Ribbon-helix-helix
Superfamily Superfamily superfamily	a.43.1 — Ribbon-helix-helix
Family Family family	a.43.1.3 — CopG-like

Domain ID domain_id	d1ea4e_
Class class	a — All alpha proteins
Fold Fold fold	a.43 — Ribbon-helix-helix
Superfamily Superfamily superfamily	a.43.1 — Ribbon-helix-helix
Family Family family	a.43.1.3 — CopG-like

Domain ID domain_id	d1ea4f_
Class class	a — All alpha proteins
Fold Fold fold	a.43 — Ribbon-helix-helix
Superfamily Superfamily superfamily	a.43.1 — Ribbon-helix-helix
Family Family family	a.43.1.3 — CopG-like

Domain ID domain_id	d1ea4g_
Class class	a — All alpha proteins
Fold Fold fold	a.43 — Ribbon-helix-helix
Superfamily Superfamily superfamily	a.43.1 — Ribbon-helix-helix
Family Family family	a.43.1.3 — CopG-like

Domain ID domain_id	d1ea4h_
Class class	a — All alpha proteins
Fold Fold fold	a.43 — Ribbon-helix-helix
Superfamily Superfamily superfamily	a.43.1 — Ribbon-helix-helix
Family Family family	a.43.1.3 — CopG-like

Domain ID domain_id	d1ea4j_
Class class	a — All alpha proteins
Fold Fold fold	a.43 — Ribbon-helix-helix
Superfamily Superfamily superfamily	a.43.1 — Ribbon-helix-helix
Family Family family	a.43.1.3 — CopG-like

Domain ID domain_id	d1ea4k_
Class class	a — All alpha proteins
Fold Fold fold	a.43 — Ribbon-helix-helix
Superfamily Superfamily superfamily	a.43.1 — Ribbon-helix-helix
Family Family family	a.43.1.3 — CopG-like

Domain ID domain_id	d1ea4l_
Class class	a — All alpha proteins
Fold Fold fold	a.43 — Ribbon-helix-helix
Superfamily Superfamily superfamily	a.43.1 — Ribbon-helix-helix
Family Family family	a.43.1.3 — CopG-like

CATH v4.4 (10 domains)

Domain ID domain_id	1ea4A00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1220 — Arc Repressor Mutant
Homologous superfamily homologous superfamily	10 — Met repressor-like

Domain ID domain_id	1ea4B00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1220 — Arc Repressor Mutant
Homologous superfamily homologous superfamily	10 — Met repressor-like

Domain ID domain_id	1ea4D00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1220 — Arc Repressor Mutant
Homologous superfamily homologous superfamily	10 — Met repressor-like

Domain ID domain_id	1ea4E00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1220 — Arc Repressor Mutant
Homologous superfamily homologous superfamily	10 — Met repressor-like

Domain ID domain_id	1ea4F00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1220 — Arc Repressor Mutant
Homologous superfamily homologous superfamily	10 — Met repressor-like

Domain ID domain_id	1ea4G00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1220 — Arc Repressor Mutant
Homologous superfamily homologous superfamily	10 — Met repressor-like

Domain ID domain_id	1ea4H00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1220 — Arc Repressor Mutant
Homologous superfamily homologous superfamily	10 — Met repressor-like

Domain ID domain_id	1ea4J00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1220 — Arc Repressor Mutant
Homologous superfamily homologous superfamily	10 — Met repressor-like

Domain ID domain_id	1ea4K00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1220 — Arc Repressor Mutant
Homologous superfamily homologous superfamily	10 — Met repressor-like

Domain ID domain_id	1ea4L00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1220 — Arc Repressor Mutant
Homologous superfamily homologous superfamily	10 — Met repressor-like

▶

Unit-cell length a length_a	213.400 Å
Unit-cell length b length_b	76.040 Å
Unit-cell length c length_c	50.520 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	40

Space group space_group	P 21 21 2
Space-group number int_tables_number	18
Crystal system crystal_system	—

Resolution resolution	2.950 Å
Low-resolution limit resolution_low	40.000 Å
R factor r_factor	0.2300
R_work r_work	0.2300
R_free r_free	0.3070

TRANSCRIPTIONAL REPRESSOR COPG/22bp dsDNA COMPLEX

1. Protein Identity and Related Structures Protein Identity & Related Structures

TRANSCRIPTIONAL REPRESSOR COPG

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1ea4__assembly_3__model_1

1ea4__assembly_3__model_1 | I(q)

1ea4__assembly_3__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Entity 4 Entity 4 water

6. Fold Classification (SCOP + CATH) 20 domains

SCOP 2.08 (10 domains)

CATH v4.4 (10 domains)

7. Citations (3)

TRANSCRIPTIONAL REPRESSOR COPG/22bp dsDNA COMPLEX

1. Protein Identity and Related Structures Protein Identity & Related Structures

TRANSCRIPTIONAL REPRESSOR COPG

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1ea4__assembly_3__model_1

1ea4__assembly_3__model_1 | I(q)

1ea4__assembly_3__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Entity 4 Entity 4 water

6. Fold Classification (SCOP + CATH) 20 domains

SCOP 2.08 (10 domains)

CATH v4.4 (10 domains)

7. Citations (3)