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1ecr

ESCHERICHIA COLI REPLICATION TERMINATOR PROTEIN (TUS) COMPLEXED WITH DNA

Method: X-RAY DIFFRACTION D_max: 77.7 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1ecr
deposition_date	1996-09-01
title	ESCHERICHIA COLI REPLICATION TERMINATOR PROTEIN (TUS) COMPLEXED WITH DNA
keywords	DNA-BINDING, DNA REPLICATION, COMPLEX (DNA-BINDING PROTEIN-DNA), REPLICATION-DNA COMPLEX; REPLICATION/DNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	22.92 Å
rg_electron	22.41 Å
i0	41582900.00
molecular_weight	44429.0 kDa
excluded_volume	53352 Å³
envelope_volume	66056 Å³
shell_volume	24754 Å³
envelope_diameter	77.6 Å
shell_rg	29.22 Å
envelope_rg	22.43 Å
shape_rg	22.43 Å
total_rg	23.13 Å
total_atoms	3103
n_residues	335
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	77.7 Å
rg_real	22.87 Å
rg_real_error	0.46 Å
i0_real	4.1580e+07
i0_real_error	6.3620e+05
rg_reciprocal	22.89 Å
i0_reciprocal	41580000.0000
total_estimate	0.8758
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	27.5 Å
skewness	0.331
kurtosis	-0.176
angular_range	— – 0.3450 Å⁻¹
current_alpha	0.0002
highest_alpha	7304000.0000
n_real_points	66
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.799; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.996; Smooth: 0.989

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	;DNA (5'-D(TPTPAPGPTPTPAPCPAPAPCPAPTPAPCPT)-3 ;
formula_weight	4856.2 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	B
atom_count	3152

TTAGTTACAACATACT

Entity 2 polymer

description	;DNA (5'-D(TPAPGPTPAPTPGPTPTPGPTPAPAPCPTPA)-3 ;
formula_weight	4927.2 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	C

TAGTATGTTGTAACTA

Entity 3 polymer

description	PROTEIN (REPLICATION-TERMINATOR PROTEIN)
formula_weight	35843.2 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A

MARYDLVDRLNTTFRQMEQELAIFAAHLEQHKLLVARVFSLPEVKKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQVDNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLTVLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQDIAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGELLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD

Source

organism	Escherichia coli
ncbi_taxonomy_id	37762
host_organism	Escherichia coli

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	49

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6. Citations (2)

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length_a	68.150 Å
length_b	68.150 Å
length_c	230.720 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	P 41 21 2
int_tables_number	92
crystal_system	—

resolution	2.700 Å
resolution_low	10.000 Å
r_factor	0.1960
r_work	0.1960
r_free	0.3140

fit	/app/data/saxs_computation/ec/1ecr/1ecr.fit
pddf	/app/data/pr_computation/ec/1ecr/1ecr.pr.out
profile	/app/data/saxs_computation/ec/1ecr/1ecr.dat

ESCHERICHIA COLI REPLICATION TERMINATOR PROTEIN (TUS) COMPLEXED WITH DNA

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Source

Entity 4 water

6. Citations (2)

7. Files & Curves (10)

Files (7)

Curves (3)