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1efz

;MUTAGENESIS AND CRYSTALLOGRAPHIC STUDIES OF ZYMOMONAS MOBILIS TRNA-GUANINE TRANSGLYCOSYLASE TO ELUCIDATE THE ROLE OF SERINE 103 FOR ENZYMATIC ACTIVITY ;

Method: X-RAY DIFFRACTION D_max: 70.0 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1efz
deposition_date	2000-02-11
title	;MUTAGENESIS AND CRYSTALLOGRAPHIC STUDIES OF ZYMOMONAS MOBILIS TRNA-GUANINE TRANSGLYCOSYLASE TO ELUCIDATE THE ROLE OF SERINE 103 FOR ENZYMATIC ACTIVITY ;
keywords	TRNA-MODIFYING ENZYME, TRANSFERASE, GLYCOSYLTRANSFERASE; TRANSFERASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	21.59 Å
rg_electron	20.46 Å
i0	31123700.00
molecular_weight	41561.0 kDa
excluded_volume	51433 Å³
envelope_volume	58507 Å³
shell_volume	23473 Å³
envelope_diameter	72.4 Å
shell_rg	27.50 Å
envelope_rg	20.67 Å
shape_rg	20.47 Å
total_rg	21.28 Å
total_atoms	2914
n_residues	372
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	70.0 Å
rg_real	21.46 Å
rg_real_error	0.40 Å
i0_real	3.1120e+07
i0_real_error	3.6960e+05
rg_reciprocal	21.48 Å
i0_reciprocal	31120000.0000
total_estimate	0.8940
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	27.8 Å
skewness	0.158
kurtosis	-0.491
angular_range	— – 0.3700 Å⁻¹
current_alpha	0.0000
highest_alpha	11010000.0000
n_real_points	69
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.877; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.987

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	TRNA-GUANINE TRANSGLYCOSYLASE
formula_weight	42909.7 kDa
ec_number	2.4.2.29
mutation	S103A
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	3276

MVEATAQETDRPRFSFSIAAREGKARTGTIEMKRGVIRTPAFMPVGTAATVKALKPETVRATGADIILGNTYHLMLRPGAERIAKLGGLHSFMGWDRPILTDAGGYQVMSLSSLTKQSEEGVTFKSHLDGSRHMLSPERSIEIQHLLGSDIVMAFDECTPYPATPSRAASSMERSMRWAKRSRDAFDSRKEQAENAALFGIQQGSVFENLRQQSADALAEIGFDGYAVGGLAVGEGQDEMFRVLDFSVPMLPDDKPHYLMGVGKPDDIVGAVERGIDMFDCVLPTRSGRNGQAFTWDGPINIRNARFSEDLKPLDSECHCAVCQKWSRAYIHHLIRAGEILGAMLMTEHNIAFYQQLMQKIRDSISEGRFSQFAQDFRARYFARNS

Source

organism	Zymomonas mobilis
ncbi_taxonomy_id	542
host_organism	Escherichia coli

Entity 2 non-polymer

description	ZINC ION
formula_weight	65.4 kDa
src_method	syn
entity_count	1

Entity 3 non-polymer

description	7-DEAZA-7-AMINOMETHYL-GUANINE
formula_weight	179.2 kDa
src_method	syn
entity_count	1

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	362

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6. Citations (2)

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length_a	90.600 Å
length_b	65.040 Å
length_c	70.740 Å
angle_alpha	90.00 °
angle_beta	96.25 °
angle_gamma	90.00 °
z_pdb	4

space_group	C 1 2 1
int_tables_number	5
crystal_system	—

resolution	2.000 Å
resolution_low	71.000 Å
r_factor	—
r_work	0.1610
r_free	0.1990

fit	/app/data/saxs_computation/ef/1efz/1efz.fit
pddf	/app/data/pr_computation/ef/1efz/1efz.pr.out
profile	/app/data/saxs_computation/ef/1efz/1efz.dat

;MUTAGENESIS AND CRYSTALLOGRAPHIC STUDIES OF ZYMOMONAS MOBILIS TRNA-GUANINE TRANSGLYCOSYLASE TO ELUCIDATE THE ROLE OF SERINE 103 FOR ENZYMATIC ACTIVITY ;

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction