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1ek6

STRUCTURE OF HUMAN UDP-GALACTOSE 4-EPIMERASE COMPLEXED WITH NADH AND UDP-GLUCOSE

Method: X-RAY DIFFRACTION D_max: 93.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1ek6
deposition_date	2000-03-06
title	STRUCTURE OF HUMAN UDP-GALACTOSE 4-EPIMERASE COMPLEXED WITH NADH AND UDP-GLUCOSE
keywords	Epimerase, Short-Chain Dehydrogenase, Galactosemia, ISOMERASE; ISOMERASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	28.32 Å
rg_electron	27.59 Å
i0	103374000.00
molecular_weight	78664.0 kDa
excluded_volume	97734 Å³
envelope_volume	113230 Å³
shell_volume	34089 Å³
envelope_diameter	99.5 Å
shell_rg	35.11 Å
envelope_rg	27.75 Å
shape_rg	27.59 Å
total_rg	28.31 Å
total_atoms	5519
n_residues	691
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	93.5 Å
rg_real	28.35 Å
rg_real_error	0.62 Å
i0_real	1.0340e+08
i0_real_error	1.4970e+06
rg_reciprocal	28.34 Å
i0_reciprocal	103400000.0000
total_estimate	0.8859
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	30.1 Å
skewness	0.377
kurtosis	-0.432
angular_range	— – 0.2800 Å⁻¹
current_alpha	0.0000
highest_alpha	32770000.0000
n_real_points	57
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.857; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.968; Smooth: 0.974

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4. Crystallography & Experiment

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5. Entities & Polymer Info (6)

Entity 1 polymer

description	UDP-GALACTOSE 4-EPIMERASE
formula_weight	38324.6 kDa
ec_number	5.1.3.2
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	6489

MAEKVLVTGGAGYIGSHTVLELLEAGYLPVVIDNFHNAFRGGGSLPESLRRVQELTGRSVEFEEMDILDQGALQRLFKKYSFMAVIHFAGLKAVGESVQKPLDYYRVNLTGTIQLLEIMKAHGVKNLVFSSSATVYGNPQYLPLDEAHPTGGCTNPYGKSKFFIEEMIRDLCQADKTWNAVLLRYFNPTGAHASGCIGEDPQGIPNNLMPYVSQVAIGRREALNVFGNDYDTEDGTGVRDYIHVVDLAKGHIAALRKLKEQCGCRIYNLGTGTGYSVLQMVQAMEKASGKKIPYKVVARREGDVAACYANPSLAQEELGWTAALGLDRMCEDLWRWQKQNPSGFGTQA

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Saccharomyces cerevisiae

Entity 2 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	2

Entity 3 non-polymer

description	1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE
formula_weight	665.4 kDa
src_method	syn
entity_count	2

Entity 4 non-polymer

description	URIDINE-5'-DIPHOSPHATE-GLUCOSE
formula_weight	566.3 kDa
src_method	syn
entity_count	2

Entity 5 non-polymer

description	TETRAMETHYLAMMONIUM ION
formula_weight	74.1 kDa
src_method	syn
entity_count	3

Entity 6 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	899

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6. Citations (1)

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length_a	74.700 Å
length_b	86.900 Å
length_c	95.300 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	1.500 Å
resolution_low	30.000 Å
r_factor	0.1690
r_work	0.1680
r_free	0.1980

fit	/app/data/saxs_computation/ek/1ek6/1ek6.fit
pddf	/app/data/pr_computation/ek/1ek6/1ek6.pr.out
profile	/app/data/saxs_computation/ek/1ek6/1ek6.dat