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1ek8

CRYSTAL STRUCTURE OF THE RIBOSOME RECYCLING FACTOR (RRF) FROM ESCHERICHIA COLI

Method: X-RAY DIFFRACTION D_max: 92.8 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1ek8
deposition_date	2000-03-07
title	CRYSTAL STRUCTURE OF THE RIBOSOME RECYCLING FACTOR (RRF) FROM ESCHERICHIA COLI
keywords	ribosome, translation factor, t-RNA mimicry, coiled coil, TRANSLATION; TRANSLATION
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	26.70 Å
rg_electron	26.75 Å
i0	9126880.00
molecular_weight	21335.0 kDa
excluded_volume	26012 Å³
envelope_volume	34482 Å³
shell_volume	12863 Å³
envelope_diameter	92.1 Å
shell_rg	28.82 Å
envelope_rg	27.03 Å
shape_rg	26.70 Å
total_rg	27.16 Å
total_atoms	1453
n_residues	185
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	92.8 Å
rg_real	27.26 Å
rg_real_error	1.18 Å
i0_real	9.1270e+06
i0_real_error	1.3850e+05
rg_reciprocal	27.09 Å
i0_reciprocal	9126000.0000
total_estimate	0.6825
solution_quality	REASONABLE a REASONABLE solution
n_peaks	3
r_peak_primary	15.7 Å
skewness	0.560
kurtosis	-0.567
angular_range	— – 0.2950 Å⁻¹
current_alpha	0.0000
highest_alpha	653100.0000
n_real_points	60
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.323; Stabil: 0.998; Sysdev: 1.000; Positv: 1.000; Valcen: 0.051; Smooth: 0.854

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	RIBOSOME RECYCLING FACTOR
formula_weight	20671.6 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	1683

MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEYYGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPLPPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDVQKLTDAAIKKIEAALADKEAELMQF

Source

organism	Escherichia coli
ncbi_taxonomy_id	562
host_organism	Escherichia coli

Entity 2 non-polymer

description	MERCURY (II) ION
formula_weight	200.6 kDa
src_method	syn
entity_count	3

Entity 3 non-polymer

description	DECYLOXY-METHANOL
formula_weight	188.3 kDa
src_method	syn
entity_count	1

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	230

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6. Citations (2)

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length_a	48.064 Å
length_b	48.064 Å
length_c	142.273 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	6

space_group	P 31 2 1
int_tables_number	152
crystal_system	—

resolution	2.300 Å
resolution_low	20.000 Å
r_factor	0.2350
r_work	0.2280
r_free	0.2970

fit	/app/data/saxs_computation/ek/1ek8/1ek8.fit
pddf	/app/data/pr_computation/ek/1ek8/1ek8.pr.out
profile	/app/data/saxs_computation/ek/1ek8/1ek8.dat

CRYSTAL STRUCTURE OF THE RIBOSOME RECYCLING FACTOR (RRF) FROM ESCHERICHIA COLI

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction