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1en7

ENDONUCLEASE VII (ENDOVII) FROM PHAGE T4

Method: X-RAY DIFFRACTION D_max: 94.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1en7
deposition_date	1999-02-07
title	ENDONUCLEASE VII (ENDOVII) FROM PHAGE T4
keywords	ENDONUCLEASE, RESOLVASE, HOLLIDAY JUNCTION, DNASE, HYDROLASE; HYDROLASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	26.06 Å
rg_electron	25.97 Å
i0	23987000.00
molecular_weight	36547.0 kDa
excluded_volume	45344 Å³
envelope_volume	58144 Å³
shell_volume	20737 Å³
envelope_diameter	97.0 Å
shell_rg	30.31 Å
envelope_rg	26.18 Å
shape_rg	25.97 Å
total_rg	26.49 Å
total_atoms	2549
n_residues	314
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	94.8 Å
rg_real	26.31 Å
rg_real_error	1.08 Å
i0_real	2.3990e+07
i0_real_error	4.0300e+05
rg_reciprocal	26.23 Å
i0_reciprocal	23990000.0000
total_estimate	0.8242
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	24.9 Å
skewness	0.558
kurtosis	-0.068
angular_range	— – 0.3050 Å⁻¹
current_alpha	0.0000
highest_alpha	4023000.0000
n_real_points	62
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.708; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.609; Smooth: 0.977

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	RECOMBINATION ENDONUCLEASE VII
formula_weight	18173.8 kDa
ec_number	3.1.22.4
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	2622

MLLTGKLYKEEKQKFYDAQNGKCLICQRELNPDVQANHLDHDHELNGPKAGKVRGLLCNLCNAAEGQMKHKFNRSGLKGQGVDYLEWLENLLTYLKSDYTQNNIHPNFVGDKSKEFSRLGKEEMMAEMLQRGFEYNESDTKTQLIASFKKQLRKSLK

Source

organism	Enterobacteria phage T4
ncbi_taxonomy_id	10665
host_organism	Escherichia coli BL21

Entity 2 non-polymer

description	ZINC ION
formula_weight	65.4 kDa
src_method	syn
entity_count	2

Entity 3 non-polymer

description	CALCIUM ION
formula_weight	40.1 kDa
src_method	syn
entity_count	3

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	73

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6. Citations (1)

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length_a	144.990 Å
length_b	39.450 Å
length_c	75.750 Å
angle_alpha	90.00 °
angle_beta	106.20 °
angle_gamma	90.00 °
z_pdb	8

space_group	C 1 2 1
int_tables_number	5
crystal_system	—

resolution	2.400 Å
resolution_low	10.000 Å
r_factor	—
r_work	0.2395
r_free	0.3080

fit	/app/data/saxs_computation/en/1en7/1en7.fit
pddf	/app/data/pr_computation/en/1en7/1en7.pr.out
profile	/app/data/saxs_computation/en/1en7/1en7.dat

ENDONUCLEASE VII (ENDOVII) FROM PHAGE T4

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction