Crystal structure of free BglII

Method: X-RAY DIFFRACTION D_max: 55.9 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1es8
deposition_date	2000-04-07
title	Crystal structure of free BglII
keywords	RESTRICTION ENDONUCLEASE, RESTRICTION ENZYME, uncomplexed, HYDROLASE; HYDROLASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	17.72 Å
rg_electron	16.58 Å
i0	9173330.00
molecular_weight	22783.0 kDa
excluded_volume	28665 Å³
envelope_volume	32794 Å³
shell_volume	16519 Å³
envelope_diameter	54.5 Å
shell_rg	22.61 Å
envelope_rg	16.90 Å
shape_rg	16.60 Å
total_rg	17.55 Å
total_atoms	1596
n_residues	193
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	55.9 Å
rg_real	17.61 Å
rg_real_error	0.27 Å
i0_real	9.1730e+06
i0_real_error	1.0890e+05
rg_reciprocal	17.62 Å
i0_reciprocal	9173000.0000
total_estimate	0.8871
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	22.4 Å
skewness	0.173
kurtosis	-0.377
angular_range	— – 0.4500 Å⁻¹
current_alpha	0.0000
highest_alpha	2262000.0000
n_real_points	76
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.869; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.996; Smooth: 0.926

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

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6. Citations (1)

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length_a	79.150 Å
length_b	79.150 Å
length_c	66.520 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	6

space_group	P 32 2 1
int_tables_number	154
crystal_system	—

resolution	2.300 Å
resolution_low	20.000 Å
r_factor	—
r_work	0.2140
r_free	0.2580

description	RESTRICTION ENDONUCLEASE BGLII
formula_weight	26031.8 kDa
ec_number	3.1.21.4
src_method	man
entity_count	1

type	polypeptide(L)
strand_id	A
atom_count	1677

Crystal structure of free BglII

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 non-polymer

Entity 3 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	Bacillus subtilis
ncbi_taxonomy_id	1423
host_organism	Escherichia coli

description	ACETIC ACID
formula_weight	60.1 kDa
src_method	syn
entity_count	1

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	81

fit	/app/data/saxs_computation/es/1es8/1es8.fit
pddf	/app/data/pr_computation/es/1es8/1es8.pr.out
profile	/app/data/saxs_computation/es/1es8/1es8.dat