HIGH RESOLUTION STRUCTURE OF THE PVUII ENDONCULEASE/COGNATE DNA COMPLEX AT PH 4.6

entry_id	1eyu
deposition_date	2000-05-09
title	HIGH RESOLUTION STRUCTURE OF THE PVUII ENDONCULEASE/COGNATE DNA COMPLEX AT PH 4.6
keywords	PROTEIN-DNA COMPLEX, endonuclease type II, restriction enzyme, hydrolase-DNA COMPLEX; hydrolase/DNA
method	X-RAY DIFFRACTION

rg_guinier	20.97 Å
rg_electron	20.44 Å
i0	36652300.00
molecular_weight	43247.0 kDa
excluded_volume	52430 ų
envelope_volume	60674 ų
shell_volume	24220 ų
envelope_diameter	66.7 Å
shell_rg	27.40 Å
envelope_rg	20.51 Å
shape_rg	20.44 Å
total_rg	21.19 Å
total_atoms	3034
n_residues	335
n_harmonics	20
q_range	— – 0.5000 Å−1
n_points	101
shell_type	directional
solvent_density	0.3340 e/ų
contrast_shell	0.0300 e/ų
crysol_version	4.1.3

dmax	63.4 Å
rg_real	20.84 Å
rg_real_error	0.24 Å
i0_real	3.6650e+07
i0_real_error	4.2200e+05
rg_reciprocal	20.86 Å
i0_reciprocal	36650000.0000
total_estimate	0.9077
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	25.7 Å
skewness	0.172
kurtosis	-0.463
angular_range	— – 0.3800 Å−1
current_alpha	0.0000
highest_alpha	7172000.0000
n_real_points	70
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.938; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.986; Smooth: 0.996

Unit Cell

length_a	93.797 Å
length_b	84.336 Å
length_c	46.155 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

Symmetry

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

Refinement

resolution	1.780 Å
resolution_low	25.000 Å
r_factor	0.2400
r_work	0.2080
r_free	0.2430

Diffraction

temperature

95.0 K

Files (7)

• pr_distribution /app/data/pr_computation/ey/1eyu/pr_distribution.json
• pr_output /app/data/pr_computation/ey/1eyu/1eyu.pr.out
• pr_plot /app/data/visualization/pr_computation/ey/1eyu/1eyu_pr_distribution.png
• saxs_curve_dat /app/data/saxs_computation/ey/1eyu/1eyu.dat
• saxs_fit /app/data/saxs_computation/ey/1eyu/1eyu.fit
• saxs_log /app/data/saxs_computation/ey/1eyu/1eyu.log
• saxs_plot /app/data/visualization/saxs_computation/ey/1eyu/1eyu_saxs_profile.png

Curves (3)

fit	/app/data/saxs_computation/ey/1eyu/1eyu.fit
pddf	/app/data/pr_computation/ey/1eyu/1eyu.pr.out
profile	/app/data/saxs_computation/ey/1eyu/1eyu.dat