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1f1s

CRYSTAL STRUCTURE OF STREPTOCOCCUS AGALACTIAE HYALURONATE LYASE AT 2.1 ANGSTROM RESOLUTION.

Method: X-RAY DIFFRACTION D_max: 110.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1f1s
deposition_date	2000-05-19
title	CRYSTAL STRUCTURE OF STREPTOCOCCUS AGALACTIAE HYALURONATE LYASE AT 2.1 ANGSTROM RESOLUTION.
keywords	;The structure consists of three distinct structural domains: two beta domains at two terminals and one alpha domain in the middle of the sequence., LYASE ;; LYASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	31.38 Å
rg_electron	31.20 Å
i0	136028000.00
molecular_weight	92387.0 kDa
excluded_volume	115360 Å³
envelope_volume	141550 Å³
shell_volume	38783 Å³
envelope_diameter	116.6 Å
shell_rg	37.23 Å
envelope_rg	31.41 Å
shape_rg	31.18 Å
total_rg	31.76 Å
total_atoms	6513
n_residues	814
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	110.5 Å
rg_real	31.57 Å
rg_real_error	1.00 Å
i0_real	1.3600e+08
i0_real_error	2.3780e+06
rg_reciprocal	31.49 Å
i0_reciprocal	136000000.0000
total_estimate	0.8389
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	33.1 Å
skewness	0.564
kurtosis	-0.029
angular_range	— – 0.2500 Å⁻¹
current_alpha	0.0000
highest_alpha	22030000.0000
n_real_points	51
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.685; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.960; Smooth: 0.887

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

Entity 1 polymer

description	HYALURONATE LYASE
formula_weight	92510.3 kDa
ec_number	4.2.2.1
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	7051

SEHPQPVTTQIEKSVNTALNKNYVFNKADYQYTLTNPSLGKIVGGILYPNATGSTTVKISDKSGKIIKEVPLSVTASTEDNFTKLLDKWNDVTIGNYVYDTNDSNMQKLNQKLDETNAKNIEAIKLDSNRTFLWKDLDNLNNSAQLTATYRRLEDLAKQITNPHSTIYKNEKAIRTVKESLAWLHQNFYNVNKDIEGSANWWDFEIGVPRSITGTLSLMNNYFTDAEIKTYTDPIEHFVPDAEYFRKTLVNPFKALGGNLVDMGRVKIIEGLLRKDNTIIEKTSHSLKNLFTTATKAEGFYADGSYIDHTNVAYTGAYGNVLIDGLTQLLPIIQETDYKISNQELDMVYKWINQSFLPLIVKGELMDMSRGRSISREAASSHAAAVEVLRGFLRLANMSNEERNLDLKSTIKTIITSNKFYNVFNNLKSYSDIANMNKLLNDSTVATKPLKSNLSTFNSMDRLAYYNAKKDFGFALSLHSKRTLNYEGMNDENTRGWYTGDGMFYIYNSDQSHYSNHFWPTVNPYKMAGTTEKDAKREDTTKEFMSKHSKDAKEKTGQVTGTSDFVGSVKLNDHFALAAMDFTNWDRTLTAQKGWVILNDKIVFLGSNIKNTNGIGNVSTTIDQRKDDSKTPYTTYVNGKTIDLKQASSQQFTDTKSVFLESKEPGRNIGYIFFKNSTIDIERKEQTGTWNSINRTSKNTSIVSNPFITISQKHDNKGDSYGYMMVPNIDRTSFDKLANSKEVELLENSSKQQVIYDKNSQTWAVIKHDNQESLINNQFKMNKAGLYLVQKVGNDYQNVYYQPQTMTKTDQLAI

Source

organism	Streptococcus agalactiae
ncbi_taxonomy_id	1311
host_organism	Escherichia coli

Entity 2 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	538

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6. Citations (1)

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length_a	51.265 Å
length_b	156.765 Å
length_c	237.429 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	C 2 2 21
int_tables_number	20
crystal_system	—

resolution	2.100 Å
resolution_low	20.000 Å
r_factor	—
r_work	0.1908
r_free	0.2530

fit	/app/data/saxs_computation/f1/1f1s/1f1s.fit
pddf	/app/data/pr_computation/f1/1f1s/1f1s.pr.out
profile	/app/data/saxs_computation/f1/1f1s/1f1s.dat

CRYSTAL STRUCTURE OF STREPTOCOCCUS AGALACTIAE HYALURONATE LYASE AT 2.1 ANGSTROM RESOLUTION.

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)