1f3w

RECOMBINANT RABBIT MUSCLE PYRUVATE KINASE

Method: X-RAY DIFFRACTION

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

PYRUVATE KINASE

Oryctolagus cuniculus

UniProt P11974

当前结构中的状态

Assembly	物理组成	蛋白状态	分子拷贝数	共同组分	数据核对
1	蛋白同源多聚体	同源多聚体	蛋白 4	POTASSIUM ION × 4 MANGANESE (II) ION × 4 PYRUVIC ACID × 4	与蛋白数一致
2	蛋白同源多聚体	同源多聚体	蛋白 4	POTASSIUM ION × 4 MANGANESE (II) ION × 4 PYRUVIC ACID × 4	与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据

UniProt名称	KPYM_RABIT
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 1–530； UniProt 1–530 作者链 B； PDB构建体 1–530； UniProt 1–530 作者链 C； PDB构建体 1–530； UniProt 1–530 作者链 D； PDB构建体 1–530； UniProt 1–530 作者链 E； PDB构建体 1–530； UniProt 1–530 作者链 F； PDB构建体 1–530； UniProt 1–530 作者链 G； PDB构建体 1–530； UniProt 1–530 作者链 H； PDB构建体 1–530； UniProt 1–530

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	1f3w
沉积日期 deposition_date	2000-06-06
结构标题 title	RECOMBINANT RABBIT MUSCLE PYRUVATE KINASE
关键词 keywords	pyruvate kinase, recombinant, muscle isozyme, TRANSFERASE; TRANSFERASE
实验方法 method	X-RAY DIFFRACTION

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3. Official assembly/model 理论 SAXS Official SAXS Profiles

页面只读取最新 assembly 计算结果。每一条曲线都对应明确的 PDB、official biological assembly 和 coordinate model。

1f3w__assembly_2__model_1

Assembly 2 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

下载该曲线 .dat

1f3w__assembly_2__model_1 | I(q)

100 个绘图点；横轴和纵轴均采用对数尺度。

1f3w__assembly_2__model_1 | P(r) · Pending

P(r) 将按相同 assembly/model 分别计算和展示。

Rg（Guinier）	40.70 Å
Rg（电子密度）	40.56 Å
总 Rg	40.79 Å
原子数	15864
残基数	2076
排除体积	283600 Å³
最大 q	0.500 Å⁻¹

Assembly	Model	结构单元	聚集说明	状态	CRYSOL	操作
1	1	`1f3w__assembly_1__model_1`	tetrameric （4）	成功	4.1.3-1-20251215 (887e7ef)	查看下载
2	1	`1f3w__assembly_2__model_1`	tetrameric （4）	成功	4.1.3-1-20251215 (887e7ef)	查看下载

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合物信息 Entities & Polymers (4)

实体 1 Entity 1 polymer

描述 description	PYRUVATE KINASE
分子量 formula_weight	57982.8 kDa
EC 编号 ec_number	2.7.1.40
来源方法 src_method	man
拷贝数 entity_count	8

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D,E,F,G,H
原子数 atom_count	31728

SKSHSEAGSAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVETLKEMIKSGMNVARMNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVDVGSKVYVDDGLISLQVKQKGPDFLVTEVENGGFLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKILGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMIIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAMFHRKLFEELARASSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTRNHQTARQAHLYRGIFPVVCKDPVQEAWAEDVDLRVNLAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP

来源 Source

来源生物 organism	Oryctolagus cuniculus
通用名 common_name	rabbit
NCBI 分类编号 ncbi_taxonomy_id	9986
宿主生物 host_organism	Escherichia coli

实体 2 Entity 2 non-polymer

描述 description	POTASSIUM ION
分子量 formula_weight	39.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	8

实体 3 Entity 3 non-polymer

描述 description	MANGANESE (II) ION
分子量 formula_weight	54.9 kDa
来源方法 src_method	syn
拷贝数 entity_count	8

实体 4 Entity 4 non-polymer

描述 description	PYRUVIC ACID
分子量 formula_weight	88.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	8

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6. 折叠分类 (SCOP + CATH) 48 domains

SCOP 2.08 (24 domains)

结构域编号 domain_id	d1f3wa1
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.58 — PK beta-barrel domain-like
超家族 Superfamily superfamily	b.58.1 — PK beta-barrel domain-like
家族 Family family	b.58.1.1 — Pyruvate kinase beta-barrel domain

结构域编号 domain_id	d1f3wa2
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamily	c.1.12 — Phosphoenolpyruvate/pyruvate domain
家族 Family family	c.1.12.1 — Pyruvate kinase

结构域编号 domain_id	d1f3wa3
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.49 — Pyruvate kinase C-terminal domain-like
超家族 Superfamily superfamily	c.49.1 — PK C-terminal domain-like
家族 Family family	c.49.1.1 — Pyruvate kinase, C-terminal domain

结构域编号 domain_id	d1f3wb1
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.58 — PK beta-barrel domain-like
超家族 Superfamily superfamily	b.58.1 — PK beta-barrel domain-like
家族 Family family	b.58.1.1 — Pyruvate kinase beta-barrel domain

结构域编号 domain_id	d1f3wb2
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamily	c.1.12 — Phosphoenolpyruvate/pyruvate domain
家族 Family family	c.1.12.1 — Pyruvate kinase

结构域编号 domain_id	d1f3wb3
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.49 — Pyruvate kinase C-terminal domain-like
超家族 Superfamily superfamily	c.49.1 — PK C-terminal domain-like
家族 Family family	c.49.1.1 — Pyruvate kinase, C-terminal domain

结构域编号 domain_id	d1f3wc1
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.58 — PK beta-barrel domain-like
超家族 Superfamily superfamily	b.58.1 — PK beta-barrel domain-like
家族 Family family	b.58.1.1 — Pyruvate kinase beta-barrel domain

结构域编号 domain_id	d1f3wc2
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamily	c.1.12 — Phosphoenolpyruvate/pyruvate domain
家族 Family family	c.1.12.1 — Pyruvate kinase

结构域编号 domain_id	d1f3wc3
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.49 — Pyruvate kinase C-terminal domain-like
超家族 Superfamily superfamily	c.49.1 — PK C-terminal domain-like
家族 Family family	c.49.1.1 — Pyruvate kinase, C-terminal domain

结构域编号 domain_id	d1f3wd1
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.58 — PK beta-barrel domain-like
超家族 Superfamily superfamily	b.58.1 — PK beta-barrel domain-like
家族 Family family	b.58.1.1 — Pyruvate kinase beta-barrel domain

结构域编号 domain_id	d1f3wd2
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamily	c.1.12 — Phosphoenolpyruvate/pyruvate domain
家族 Family family	c.1.12.1 — Pyruvate kinase

结构域编号 domain_id	d1f3wd3
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.49 — Pyruvate kinase C-terminal domain-like
超家族 Superfamily superfamily	c.49.1 — PK C-terminal domain-like
家族 Family family	c.49.1.1 — Pyruvate kinase, C-terminal domain

结构域编号 domain_id	d1f3we1
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.58 — PK beta-barrel domain-like
超家族 Superfamily superfamily	b.58.1 — PK beta-barrel domain-like
家族 Family family	b.58.1.1 — Pyruvate kinase beta-barrel domain

结构域编号 domain_id	d1f3we2
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamily	c.1.12 — Phosphoenolpyruvate/pyruvate domain
家族 Family family	c.1.12.1 — Pyruvate kinase

结构域编号 domain_id	d1f3we3
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.49 — Pyruvate kinase C-terminal domain-like
超家族 Superfamily superfamily	c.49.1 — PK C-terminal domain-like
家族 Family family	c.49.1.1 — Pyruvate kinase, C-terminal domain

结构域编号 domain_id	d1f3wf1
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.58 — PK beta-barrel domain-like
超家族 Superfamily superfamily	b.58.1 — PK beta-barrel domain-like
家族 Family family	b.58.1.1 — Pyruvate kinase beta-barrel domain

结构域编号 domain_id	d1f3wf2
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamily	c.1.12 — Phosphoenolpyruvate/pyruvate domain
家族 Family family	c.1.12.1 — Pyruvate kinase

结构域编号 domain_id	d1f3wf3
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.49 — Pyruvate kinase C-terminal domain-like
超家族 Superfamily superfamily	c.49.1 — PK C-terminal domain-like
家族 Family family	c.49.1.1 — Pyruvate kinase, C-terminal domain

结构域编号 domain_id	d1f3wg1
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.58 — PK beta-barrel domain-like
超家族 Superfamily superfamily	b.58.1 — PK beta-barrel domain-like
家族 Family family	b.58.1.1 — Pyruvate kinase beta-barrel domain

结构域编号 domain_id	d1f3wg2
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamily	c.1.12 — Phosphoenolpyruvate/pyruvate domain
家族 Family family	c.1.12.1 — Pyruvate kinase

结构域编号 domain_id	d1f3wg3
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.49 — Pyruvate kinase C-terminal domain-like
超家族 Superfamily superfamily	c.49.1 — PK C-terminal domain-like
家族 Family family	c.49.1.1 — Pyruvate kinase, C-terminal domain

结构域编号 domain_id	d1f3wh1
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.58 — PK beta-barrel domain-like
超家族 Superfamily superfamily	b.58.1 — PK beta-barrel domain-like
家族 Family family	b.58.1.1 — Pyruvate kinase beta-barrel domain

结构域编号 domain_id	d1f3wh2
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamily	c.1.12 — Phosphoenolpyruvate/pyruvate domain
家族 Family family	c.1.12.1 — Pyruvate kinase

结构域编号 domain_id	d1f3wh3
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.49 — Pyruvate kinase C-terminal domain-like
超家族 Superfamily superfamily	c.49.1 — PK C-terminal domain-like
家族 Family family	c.49.1.1 — Pyruvate kinase, C-terminal domain

CATH v4.4 (24 domains)

结构域编号 domain_id	1f3wA01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	1380 — Pyruvate Kinase; Chain: A, domain 1
同源超家族 H-superfamily homologous superfamily	20 — Pyruvate kinase, C-terminal domain

结构域编号 domain_id	1f3wA02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	20 — Alpha-Beta Barrel
拓扑 Topology topology	20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily	60 — Phosphoenolpyruvate-binding domains

结构域编号 domain_id	1f3wA03
类 Class class	2 — Mainly Beta
架构 Architecture architecture	40 — Beta Barrel
拓扑 Topology topology	33 — M1 Pyruvate Kinase; Domain 3
同源超家族 H-superfamily homologous superfamily	10 — PK beta-barrel domain-like

结构域编号 domain_id	1f3wB01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	1380 — Pyruvate Kinase; Chain: A, domain 1
同源超家族 H-superfamily homologous superfamily	20 — Pyruvate kinase, C-terminal domain

结构域编号 domain_id	1f3wB02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	20 — Alpha-Beta Barrel
拓扑 Topology topology	20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily	60 — Phosphoenolpyruvate-binding domains

结构域编号 domain_id	1f3wB03
类 Class class	2 — Mainly Beta
架构 Architecture architecture	40 — Beta Barrel
拓扑 Topology topology	33 — M1 Pyruvate Kinase; Domain 3
同源超家族 H-superfamily homologous superfamily	10 — PK beta-barrel domain-like

结构域编号 domain_id	1f3wC01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	1380 — Pyruvate Kinase; Chain: A, domain 1
同源超家族 H-superfamily homologous superfamily	20 — Pyruvate kinase, C-terminal domain

结构域编号 domain_id	1f3wC02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	20 — Alpha-Beta Barrel
拓扑 Topology topology	20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily	60 — Phosphoenolpyruvate-binding domains

结构域编号 domain_id	1f3wC03
类 Class class	2 — Mainly Beta
架构 Architecture architecture	40 — Beta Barrel
拓扑 Topology topology	33 — M1 Pyruvate Kinase; Domain 3
同源超家族 H-superfamily homologous superfamily	10 — PK beta-barrel domain-like

结构域编号 domain_id	1f3wD01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	1380 — Pyruvate Kinase; Chain: A, domain 1
同源超家族 H-superfamily homologous superfamily	20 — Pyruvate kinase, C-terminal domain

结构域编号 domain_id	1f3wD02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	20 — Alpha-Beta Barrel
拓扑 Topology topology	20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily	60 — Phosphoenolpyruvate-binding domains

结构域编号 domain_id	1f3wD03
类 Class class	2 — Mainly Beta
架构 Architecture architecture	40 — Beta Barrel
拓扑 Topology topology	33 — M1 Pyruvate Kinase; Domain 3
同源超家族 H-superfamily homologous superfamily	10 — PK beta-barrel domain-like

结构域编号 domain_id	1f3wE01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	1380 — Pyruvate Kinase; Chain: A, domain 1
同源超家族 H-superfamily homologous superfamily	20 — Pyruvate kinase, C-terminal domain

结构域编号 domain_id	1f3wE02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	20 — Alpha-Beta Barrel
拓扑 Topology topology	20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily	60 — Phosphoenolpyruvate-binding domains

结构域编号 domain_id	1f3wE03
类 Class class	2 — Mainly Beta
架构 Architecture architecture	40 — Beta Barrel
拓扑 Topology topology	33 — M1 Pyruvate Kinase; Domain 3
同源超家族 H-superfamily homologous superfamily	10 — PK beta-barrel domain-like

结构域编号 domain_id	1f3wF01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	1380 — Pyruvate Kinase; Chain: A, domain 1
同源超家族 H-superfamily homologous superfamily	20 — Pyruvate kinase, C-terminal domain

结构域编号 domain_id	1f3wF02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	20 — Alpha-Beta Barrel
拓扑 Topology topology	20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily	60 — Phosphoenolpyruvate-binding domains

结构域编号 domain_id	1f3wF03
类 Class class	2 — Mainly Beta
架构 Architecture architecture	40 — Beta Barrel
拓扑 Topology topology	33 — M1 Pyruvate Kinase; Domain 3
同源超家族 H-superfamily homologous superfamily	10 — PK beta-barrel domain-like

结构域编号 domain_id	1f3wG01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	1380 — Pyruvate Kinase; Chain: A, domain 1
同源超家族 H-superfamily homologous superfamily	20 — Pyruvate kinase, C-terminal domain

结构域编号 domain_id	1f3wG02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	20 — Alpha-Beta Barrel
拓扑 Topology topology	20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily	60 — Phosphoenolpyruvate-binding domains

结构域编号 domain_id	1f3wG03
类 Class class	2 — Mainly Beta
架构 Architecture architecture	40 — Beta Barrel
拓扑 Topology topology	33 — M1 Pyruvate Kinase; Domain 3
同源超家族 H-superfamily homologous superfamily	10 — PK beta-barrel domain-like

结构域编号 domain_id	1f3wH01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	1380 — Pyruvate Kinase; Chain: A, domain 1
同源超家族 H-superfamily homologous superfamily	20 — Pyruvate kinase, C-terminal domain

结构域编号 domain_id	1f3wH02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	20 — Alpha-Beta Barrel
拓扑 Topology topology	20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily	60 — Phosphoenolpyruvate-binding domains

结构域编号 domain_id	1f3wH03
类 Class class	2 — Mainly Beta
架构 Architecture architecture	40 — Beta Barrel
拓扑 Topology topology	33 — M1 Pyruvate Kinase; Domain 3
同源超家族 H-superfamily homologous superfamily	10 — PK beta-barrel domain-like

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晶胞边长 a length_a	79.000 Å
晶胞边长 b length_b	105.400 Å
晶胞边长 c length_c	143.500 Å
晶胞夹角 α angle_alpha	95.90 °
晶胞夹角 β angle_beta	92.80 °
晶胞夹角 γ angle_gamma	111.90 °
晶胞分子数 Z z_pdb	8

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	3.000 Å
最低分辨率 resolution_low	52.000 Å
R 因子 r_factor	0.2380
R_work r_work	0.2380
R_free r_free	0.2570

RECOMBINANT RABBIT MUSCLE PYRUVATE KINASE

1. 蛋白身份与相关结构 Protein Identity & Related Structures

PYRUVATE KINASE

当前结构中的状态

数据库中的同蛋白其他状态

2. 结构基本信息 2. Structure Basics

3. Official assembly/model 理论 SAXS Official SAXS Profiles

1f3w__assembly_2__model_1

1f3w__assembly_2__model_1 | I(q)

1f3w__assembly_2__model_1 | P(r) · Pending

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合物信息 Entities & Polymers (4)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 non-polymer

实体 3 Entity 3 non-polymer

实体 4 Entity 4 non-polymer

6. 折叠分类 (SCOP + CATH) 48 domains

SCOP 2.08 (24 domains)

CATH v4.4 (24 domains)

7. 引用文献 (1)

RECOMBINANT RABBIT MUSCLE PYRUVATE KINASE

1. 蛋白身份与相关结构 Protein Identity & Related Structures

PYRUVATE KINASE

当前结构中的状态

数据库中的同蛋白其他状态

单体

同源二聚体

同源四聚体

其他同源多聚体

异源或核酸复合物

2. 结构基本信息 2. Structure Basics

3. Official assembly/model 理论 SAXS Official SAXS Profiles

1f3w__assembly_2__model_1

1f3w__assembly_2__model_1 | I(q)

1f3w__assembly_2__model_1 | P(r) · Pending

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合物信息 Entities & Polymers (4)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 non-polymer

实体 3 Entity 3 non-polymer

实体 4 Entity 4 non-polymer

6. 折叠分类 (SCOP + CATH) 48 domains

SCOP 2.08 (24 domains)

CATH v4.4 (24 domains)

7. 引用文献 (1)