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1f4k

CRYSTAL STRUCTURE OF THE REPLICATION TERMINATOR PROTEIN/B-SITE DNA COMPLEX

Method: X-RAY DIFFRACTION D_max: 87.3 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1f4k
deposition_date	2000-06-08
title	CRYSTAL STRUCTURE OF THE REPLICATION TERMINATOR PROTEIN/B-SITE DNA COMPLEX
keywords	winged-helix protein-DNA complex, REPLICATION AND TERMINATION, FORK ARREST MECHANISM, REPLICATION-DNA COMPLEX; REPLICATION/DNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	23.73 Å
rg_electron	23.25 Å
i0	35386600.00
molecular_weight	40126.0 kDa
excluded_volume	47705 Å³
envelope_volume	59252 Å³
shell_volume	22029 Å³
envelope_diameter	89.3 Å
shell_rg	29.35 Å
envelope_rg	23.68 Å
shape_rg	23.20 Å
total_rg	24.02 Å
total_atoms	2777
n_residues	272
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	87.3 Å
rg_real	23.84 Å
rg_real_error	0.85 Å
i0_real	3.5390e+07
i0_real_error	5.6540e+05
rg_reciprocal	23.81 Å
i0_reciprocal	35390000.0000
total_estimate	0.8298
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	27.3 Å
skewness	0.475
kurtosis	-0.167
angular_range	— – 0.3350 Å⁻¹
current_alpha	0.0000
highest_alpha	4330000.0000
n_real_points	65
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.675; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.767; Smooth: 0.994

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	5'-D(CPTPAPTPGPAPAPCPAPTPAPAPTPGPTPTPCPAPTPAP*G)-3'
formula_weight	6445.2 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	D
atom_count	2873

CTATGAACATAATGTTCATAG

Entity 2 polymer

description	5'-D(CPTPAPTPGPAPAPCPAPTPTPAPTPGPTPTPCPAPTPAP*G)-3'
formula_weight	6436.2 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	E

CTATGAACATTATGTTCATAG

Entity 3 polymer

description	REPLICATION TERMINATION PROTEIN
formula_weight	14531.1 kDa
mutation	C110S
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B

MKEEKRSSTGFLVKQRAFLKLYMITMTEQERLYGLKLLEVLRSEFKEIGFKPNHTEVYRSLHELLDDGILKQIKVKKEGAKLQEVVLYQFKDYEAAKLYKKQLKVELDRSKKLIEKALSDNF

Source

organism	Bacillus subtilis
ncbi_taxonomy_id	1423
host_organism	Escherichia coli

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	96

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6. Citations (1)

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length_a	44.780 Å
length_b	44.780 Å
length_c	395.580 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	12

space_group	P 32 2 1
int_tables_number	154
crystal_system	—

resolution	2.500 Å
resolution_low	50.000 Å
r_factor	0.2710
r_work	0.2279
r_free	0.2759

fit	/app/data/saxs_computation/f4/1f4k/1f4k.fit
pddf	/app/data/pr_computation/f4/1f4k/1f4k.pr.out
profile	/app/data/saxs_computation/f4/1f4k/1f4k.dat

CRYSTAL STRUCTURE OF THE REPLICATION TERMINATOR PROTEIN/B-SITE DNA COMPLEX

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Source

Entity 4 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)