;A TRANSIENT INTERACTION BETWEEN TWO PHOSPHORELAY PROTEINS TRAPPED IN A CRYSTAL LATTICE REVEALS THE MECHANISM OF MOLECULAR RECOGNITION AND PHOSPHOTRANSFER IN SINGAL TRANSDUCTION ;

entry_id	1f51
deposition_date	2000-06-11
title	;A TRANSIENT INTERACTION BETWEEN TWO PHOSPHORELAY PROTEINS TRAPPED IN A CRYSTAL LATTICE REVEALS THE MECHANISM OF MOLECULAR RECOGNITION AND PHOSPHOTRANSFER IN SINGAL TRANSDUCTION ;
keywords	TWO COMPONENT SYSTEM, SINGAL TRANDUCTION, RESPONSE REGULATOR, PHOSPHOTRANSFERASE, SPORULATION, PHOSPHORELAY, TRANSFERASE; TRANSFERASE
method	X-RAY DIFFRACTION

rg_guinier	39.68 Å
rg_electron	39.59 Å
i0	278512000.00
molecular_weight	138520.0 kDa
excluded_volume	174560 ų
envelope_volume	232590 ų
shell_volume	51271 ų
envelope_diameter	147.0 Å
shell_rg	43.07 Å
envelope_rg	39.05 Å
shape_rg	39.60 Å
total_rg	39.77 Å
total_atoms	9751
n_residues	1202
n_harmonics	20
q_range	— – 0.5000 Å−1
n_points	101
shell_type	directional
solvent_density	0.3340 e/ų
contrast_shell	0.0300 e/ų
crysol_version	4.1.3

dmax	137.9 Å
rg_real	40.01 Å
rg_real_error	1.43 Å
i0_real	2.7850e+08
i0_real_error	4.9570e+06
rg_reciprocal	39.81 Å
i0_reciprocal	278500000.0000
total_estimate	0.8418
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	44.8 Å
skewness	0.545
kurtosis	-0.177
angular_range	— – 0.2000 Å−1
current_alpha	0.0000
highest_alpha	35830000.0000
n_real_points	41
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.738; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.920; Smooth: 0.806

Unit Cell

length_a	72.974 Å
length_b	117.774 Å
length_c	170.736 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	16

Symmetry

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

Refinement

resolution	3.000 Å
resolution_low	45.000 Å
r_factor	0.2290
r_work	0.2280
r_free	0.2680

Diffraction

temperature

90.0 K

Files (7)

• pr_distribution /app/data/pr_computation/f5/1f51/pr_distribution.json
• pr_output /app/data/pr_computation/f5/1f51/1f51.pr.out
• pr_plot /app/data/visualization/pr_computation/f5/1f51/1f51_pr_distribution.png
• saxs_curve_dat /app/data/saxs_computation/f5/1f51/1f51.dat
• saxs_fit /app/data/saxs_computation/f5/1f51/1f51.fit
• saxs_log /app/data/saxs_computation/f5/1f51/1f51.log
• saxs_plot /app/data/visualization/saxs_computation/f5/1f51/1f51_saxs_profile.png

Curves (3)

fit	/app/data/saxs_computation/f5/1f51/1f51.fit
pddf	/app/data/pr_computation/f5/1f51/1f51.pr.out
profile	/app/data/saxs_computation/f5/1f51/1f51.dat