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1f51

A TRANSIENT INTERACTION BETWEEN TWO PHOSPHORELAY PROTEINS TRAPPED IN A CRYSTAL LATTICE REVEALS THE MECHANISM OF MOLECULAR RECOGNITION AND PHOSPHOTRANSFER IN SINGAL TRANSDUCTION

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

SPORULATION INITIATION PHOSPHOTRANSFERASE B

Bacillus subtilis

UniProt P06535

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 4 SPORULATION INITIATION PHOSPHOTRANSFERASE F × 2 （P06628） MAGNESIUM ION × 2 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 4 SPORULATION INITIATION PHOSPHOTRANSFERASE F × 2 （P06628） MAGNESIUM ION × 1 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer
1ixm

1 assemblies

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1f51 1f51 2ftk 2ftk

4 assemblies

View Construct and Data Evidence
UniProt name SP0B_BACSU
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–182； UniProt 11–192 Author chain B； PDBConstruct 1–182； UniProt 11–192 Author chain C； PDBConstruct 1–182； UniProt 11–192 Author chain D； PDBConstruct 1–182； UniProt 11–192

SPORULATION INITIATION PHOSPHOTRANSFERASE F

Bacillus subtilis

UniProt P06628

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 4 SPORULATION INITIATION PHOSPHOTRANSFERASE B × 2 （P06535） MAGNESIUM ION × 2 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 4 SPORULATION INITIATION PHOSPHOTRANSFERASE B × 2 （P06535） MAGNESIUM ION × 1 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
1fsp 1nat 1pey 1pey 1pey 1pux 2fsp 2jvi 2jvj 2jvk

10 assemblies

Homodimer
1srr 1srr

2 assemblies

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1f51 1f51 2ftk 2ftk 3q15 3q15

6 assemblies

View Construct and Data Evidence
UniProt name SP0F_BACSU
Isoform —
PDB entities 2
Chains and sequence ranges Author chain E； PDBConstruct 1–119； UniProt 3–121 Author chain F； PDBConstruct 1–119； UniProt 3–121 Author chain G； PDBConstruct 1–119； UniProt 3–121 Author chain H； PDBConstruct 1–119； UniProt 3–121

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id1f51
Deposition date deposition_date2000-06-11
Structure title titleA TRANSIENT INTERACTION BETWEEN TWO PHOSPHORELAY PROTEINS TRAPPED IN A CRYSTAL LATTICE REVEALS THE MECHANISM OF MOLECULAR RECOGNITION AND PHOSPHOTRANSFER IN SINGAL TRANSDUCTION
Keywords keywordsTWO COMPONENT SYSTEM, SINGAL TRANDUCTION, RESPONSE REGULATOR, PHOSPHOTRANSFERASE, SPORULATION, PHOSPHORELAY, TRANSFERASE; TRANSFERASE
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1f51__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1f51__assembly_1__model_1 | I(q)

10-2 10-1 106 107 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

1f51__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）27.82 Å
Rg (electron density)26.68 Å
Total Rg27.46 Å
Atom count4885
Residues601
Excluded volume87460 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 1f51__assembly_1__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 1f51__assembly_2__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (3)

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6. Fold Classification (SCOP + CATH) 20 domains

SCOP 2.08 (8 domains)

Domain ID domain_idd1f51a_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.123 — Sporulation response regulatory protein Spo0B
Superfamily Superfamily superfamilyd.123.1 — Sporulation response regulatory protein Spo0B
Family Family familyd.123.1.1 — Sporulation response regulatory protein Spo0B
Domain ID domain_idd1f51b_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.123 — Sporulation response regulatory protein Spo0B
Superfamily Superfamily superfamilyd.123.1 — Sporulation response regulatory protein Spo0B
Family Family familyd.123.1.1 — Sporulation response regulatory protein Spo0B
Domain ID domain_idd1f51c_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.123 — Sporulation response regulatory protein Spo0B
Superfamily Superfamily superfamilyd.123.1 — Sporulation response regulatory protein Spo0B
Family Family familyd.123.1.1 — Sporulation response regulatory protein Spo0B
Domain ID domain_idd1f51d_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.123 — Sporulation response regulatory protein Spo0B
Superfamily Superfamily superfamilyd.123.1 — Sporulation response regulatory protein Spo0B
Family Family familyd.123.1.1 — Sporulation response regulatory protein Spo0B
Domain ID domain_idd1f51e_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.23 — Flavodoxin-like
Superfamily Superfamily superfamilyc.23.1 — CheY-like
Family Family familyc.23.1.1 — CheY-related
Domain ID domain_idd1f51f_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.23 — Flavodoxin-like
Superfamily Superfamily superfamilyc.23.1 — CheY-like
Family Family familyc.23.1.1 — CheY-related
Domain ID domain_idd1f51g_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.23 — Flavodoxin-like
Superfamily Superfamily superfamilyc.23.1 — CheY-like
Family Family familyc.23.1.1 — CheY-related
Domain ID domain_idd1f51h_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.23 — Flavodoxin-like
Superfamily Superfamily superfamilyc.23.1 — CheY-like
Family Family familyc.23.1.1 — CheY-related

CATH v4.4 (12 domains)

Domain ID domain_id1f51A01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology287 — Helix Hairpins
Homologous superfamily homologous superfamily130 — Signal transduction histidine kinase, dimerisation/phosphotransfer (DHp) domain
Domain ID domain_id1f51A02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology565 — Heat Shock Protein 90
Homologous superfamily homologous superfamily30 — Sporulation initiation phosphotransferase B (SpoOB), C-terminal domain
Domain ID domain_id1f51B01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology287 — Helix Hairpins
Homologous superfamily homologous superfamily130 — Signal transduction histidine kinase, dimerisation/phosphotransfer (DHp) domain
Domain ID domain_id1f51B02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology565 — Heat Shock Protein 90
Homologous superfamily homologous superfamily30 — Sporulation initiation phosphotransferase B (SpoOB), C-terminal domain
Domain ID domain_id1f51C01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology287 — Helix Hairpins
Homologous superfamily homologous superfamily130 — Signal transduction histidine kinase, dimerisation/phosphotransfer (DHp) domain
Domain ID domain_id1f51C02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology565 — Heat Shock Protein 90
Homologous superfamily homologous superfamily30 — Sporulation initiation phosphotransferase B (SpoOB), C-terminal domain
Domain ID domain_id1f51D01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology287 — Helix Hairpins
Homologous superfamily homologous superfamily130 — Signal transduction histidine kinase, dimerisation/phosphotransfer (DHp) domain
Domain ID domain_id1f51D02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology565 — Heat Shock Protein 90
Homologous superfamily homologous superfamily30 — Sporulation initiation phosphotransferase B (SpoOB), C-terminal domain
Domain ID domain_id1f51E00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily2300 — Response regulator
Domain ID domain_id1f51F00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily2300 — Response regulator
Domain ID domain_id1f51G00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily2300 — Response regulator
Domain ID domain_id1f51H00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily2300 — Response regulator
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7. Citations (1)