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1f60

CRYSTAL STRUCTURE OF THE YEAST ELONGATION FACTOR COMPLEX EEF1A:EEF1BA

Method: X-RAY DIFFRACTION D_max: 83.1 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1f60
deposition_date	2000-06-19
title	CRYSTAL STRUCTURE OF THE YEAST ELONGATION FACTOR COMPLEX EEF1A:EEF1BA
keywords	protein-protein complex, TRANSLATION; TRANSLATION
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	24.81 Å
rg_electron	23.78 Å
i0	55039400.00
molecular_weight	58163.0 kDa
excluded_volume	73148 Å³
envelope_volume	87350 Å³
shell_volume	30149 Å³
envelope_diameter	82.2 Å
shell_rg	31.37 Å
envelope_rg	23.73 Å
shape_rg	23.79 Å
total_rg	24.62 Å
total_atoms	4091
n_residues	530
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	83.1 Å
rg_real	24.65 Å
rg_real_error	0.50 Å
i0_real	5.5040e+07
i0_real_error	7.3340e+05
rg_reciprocal	24.69 Å
i0_reciprocal	55040000.0000
total_estimate	0.8039
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	31.7 Å
skewness	0.177
kurtosis	-0.436
angular_range	— – 0.3200 Å⁻¹
current_alpha	0.0000
highest_alpha	16100000.0000
n_real_points	64
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.816; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.998; Smooth: 0.000

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	ELONGATION FACTOR EEF1A
formula_weight	50110.6 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	4824

MGKEKSHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYQVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGVGEFEAGISKDGQTREHALLAFTLGVRQLIVAVNKMDSVKWDESRFQEIVKETSNFIKKVGYNPKTVPFVPISGWNGDNMIEATTNAPWYKGWEKETKAGVVKGKTLLEAIDAIEQPSRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMVVTFAPAGVTTEVKSVEMHHEQLEQGVPGDNVGFNVKNVSVKEIRRGNVCGDAKNDPPKGCASFNATVIVLNHPGQISAGYSPVLDCHTAHIACRFDELLEKNDRRSGKKLEDHPKFLKSGDAALVKFVPSKPMCVEAFSEYPPLGRFAVRDMRQTVAVGVIKSVDKTEKAAKVTKAAQKAAKK

Entity 2 polymer

description	ELONGATION FACTOR EEF1BA
formula_weight	10472.8 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

KPAKPAAKSIVTLDVKPWDDETNLEEMVANVKAIEMEGLTWGAHQFIPIGFGIKKLQINCVVEDDKVSLDDLQQSIEEDEDHVQSTDIAAMQKL

Source

organism	Saccharomyces cerevisiae
common_name	;baker's yeast ;
ncbi_taxonomy_id	4932
host_organism	Escherichia coli

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	733

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6. Citations (2)

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length_a	63.850 Å
length_b	91.810 Å
length_c	92.980 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	1.670 Å
resolution_low	20.000 Å
r_factor	0.1890
r_work	0.1870
r_free	0.2210

fit	/app/data/saxs_computation/f6/1f60/1f60.fit
pddf	/app/data/pr_computation/f6/1f60/1f60.pr.out
profile	/app/data/saxs_computation/f6/1f60/1f60.dat

CRYSTAL STRUCTURE OF THE YEAST ELONGATION FACTOR COMPLEX EEF1A:EEF1BA

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Source

Entity 3 water

6. Citations (2)

7. Files & Curves (10)

Files (7)

Curves (3)