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1g68

PSE-4 CARBENICILLINASE, WILD TYPE

Method: X-RAY DIFFRACTION Dmax: 61.4 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

BETA-LACTAMASE PSE-4

Pseudomonas aeruginosa

UniProt P16897

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 18–288 Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；ammonium sulfate, MgCl2, MOPS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K Resolution 1.95 Å R-free 0.212

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 1G68 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1G6A PSE-4 CARBENICILLINASE, R234K MUTANT Deposited 2000-11-03 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 18–288（271 aa）
Mutation：R234K SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；ammonium sulfate, sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.75 Å R-free 0.209
4ID4 Crystal structure of chimeric beta-lactamase cTEM-17m Deposited 2012-12-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 145–185（41 aa）
Not recorded CL CHLORIDE ION × 5 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；26% PEG 4000, 0.25M magnesium chloride, 0.1M TrisHCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 1.05 Å R-free 0.138
4QY5 Crystal structures of chimeric beta-lactamase cTEM-19m showing different conformations Deposited 2014-07-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 63–64（2 aa）
Chain A 145–185（41 aa）
Not recorded CL CHLORIDE ION × 4 MG MAGNESIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；295 K；30% PEG 4000, 0.2M magnesium chloride, 0.1M TrisHCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 1.50 Å R-free 0.161
4QY6 Crystal structures of chimeric beta-lactamase cTEM-19m showing different conformations Deposited 2014-07-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 63–64（2 aa）
Chain A 145–185（41 aa）
Not recorded CL CHLORIDE ION × 6 MG MAGNESIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；295 K；30% PEG 4000, 0.2M magnesium chloride, 0.1M TrisHCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 1.15 Å R-free 0.139
4R4R Crystal structure of chimeric beta-lactamase cTEM-19m at 1.2 angstrom resolution Deposited 2014-08-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 63–64（2 aa）
Chain A 145–185（41 aa）
Not recorded CL CHLORIDE ION × 4 MG MAGNESIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；295 K；30% PEG 4000, 0.2M magnesium chloride, 0.1M TrisHCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 1.20 Å R-free 0.138
4R4S Crystal structure of chimeric beta-lactamase cTEM-19m at 1.1 angstrom resolution Deposited 2014-08-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 63–64（2 aa）
Chain A 145–185（41 aa）
Not recorded CL CHLORIDE ION × 5 MG MAGNESIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；295 K；30% PEG 4000, 0.2M magnesium chloride, 0.1M TrisHCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 1.10 Å R-free 0.128
8J6Y Crystal structure of class A beta-lactamase PSE-4 WT- Apo state Deposited 2023-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–288（288 aa）
Not recorded SO4 SULFATE ION × 16 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；2.5 M Ammonium sulfate, 0.1 M BIS-TRIS propane pH 7.0
Resolution 2.40 Å R-free 0.272

7 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name BLP4_PSEAE
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–271； UniProt 18–288

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 1g68

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 1g68
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2. Structure Basics 2. Structure Basics

Entry ID entry_id1g68
Deposition date deposition_date2000-11-03
Structure title titlePSE-4 CARBENICILLINASE, WILD TYPE
Keywords keywordsclass A beta-lactamase, carbenicillinase, HYDROLASE; HYDROLASE
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier18.88 Å
Radius of gyration Rg (electron density) rg_electron17.77 Å
Forward intensity I(0) i015232400.00
Molecular weight molecular_weight29056.0 kDa
Excluded volume excluded_volume36255 ų
Envelope volume envelope_volume40627 ų
Hydration-shell volume shell_volume18889 ų
Envelope diameter envelope_diameter62.2 Å
Shell Rg shell_rg24.34 Å
Envelope Rg envelope_rg18.15 Å
Shape Rg shape_rg17.77 Å
Total Rg total_rg18.70 Å
Total atoms total_atoms2043
Residues n_residues266
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax61.4 Å
Rg (real space) rg_real18.78 Å
Rg uncertainty (real space) rg_real_error0.35 Å
I(0) (real space) i0_real1.5230e+07
I(0) uncertainty (real space) i0_real_error1.8970e+05
Rg (reciprocal space) rg_reciprocal18.80 Å
I(0) (reciprocal space) i0_reciprocal15230000.0000
Solution quality estimate total_estimate0.8057
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks3
Primary peak position r_peak_primary23.9 Å
Skewness Skewness skewness0.225
Kurtosis Kurtosis kurtosis-0.290
Angular range angular_range— – 0.4200 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha3313000.0000
Real-space data points n_real_points73
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.825; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.998; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (3)

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7. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

Domain ID domain_idd1g68a_
Class classe — Multi-domain proteins (alpha and beta)
Fold Fold folde.3 — beta-lactamase/transpeptidase-like
Superfamily Superfamily superfamilye.3.1 — beta-lactamase/transpeptidase-like
Family Family familye.3.1.1 — beta-Lactamase/D-ala carboxypeptidase

CATH v4.4 (1 domains)

Domain ID domain_id1g68A00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology710 — Beta-lactamase
Homologous superfamily homologous superfamily10 — DD-peptidase/beta-lactamase superfamily
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8. Citations (1)

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9. Files and Curves (10)