1gdt

CRYSTAL STRUCTURE OF A SITE-SPECIFIC RECOMBINASE, GAMMA-DELTA RESOLVASE COMPLEXED WITH A 34 BP CLEAVAGE SITE

Method: X-RAY DIFFRACTION Dmax: 99.8 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 1gdt

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1gdt
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1gdt
沉积日期 deposition_date1995-04-11
结构标题 titleCRYSTAL STRUCTURE OF A SITE-SPECIFIC RECOMBINASE, GAMMA-DELTA RESOLVASE COMPLEXED WITH A 34 BP CLEAVAGE SITE
关键词 keywordsPROTEIN-DNA COMPLEX, DOUBLE HELIX, OVERHANGING BASE, DNA BINDING PROTEIN-DNA COMPLEX; DNA BINDING PROTEIN/DNA
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier31.92
回转半径 Rg (电子) rg_electron31.32
零角强度 I(0) i094475900.00
分子量 molecular_weight61949.0 kDa
排除体积 excluded_volume71297 ų
包络体积 envelope_volume100070 ų
水化壳体积 shell_volume28694 ų
包络直径 envelope_diameter108.2
壳层 Rg shell_rg35.42
包络 Rg envelope_rg31.23
形状 Rg shape_rg31.28
总 Rg total_rg31.73
总原子数 total_atoms4255
残基数 n_residues435
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax99.8
Rg (实空间) rg_real31.93
Rg 误差 (实空间) rg_real_error0.76
I(0) (实空间) i0_real9.4480e+07
I(0) 误差 (实空间) i0_real_error1.3250e+06
Rg (倒空间) rg_reciprocal31.93
I(0) (倒空间) i0_reciprocal94480000.0000
解质量估计 total_estimate0.8900
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks1
主峰位置 r_peak_primary97.8
偏度 Skewness skewness0.209
峰度 Kurtosis kurtosis-0.622
角度范围 angular_range— – 0.2500 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha6489000.0000
实空间数据点数 n_real_points51
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.961; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.962; Smooth: 0.719

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (5)

6. 折叠分类 (SCOP + CATH) 10 domains

SCOP 2.08 (4 domains)

结构域编号 domain_idd1gdta1
类 Class classa — All alpha proteins
折叠类型 Fold folda.4 — DNA/RNA-binding 3-helical bundle
超家族 Superfamily superfamilya.4.1 — Homeodomain-like
家族 Family familya.4.1.2 — Recombinase DNA-binding domain
结构域编号 domain_idd1gdta2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.53 — Resolvase-like
超家族 Superfamily superfamilyc.53.1 — Resolvase-like
家族 Family familyc.53.1.1 — gamma,delta resolvase, catalytic domain
结构域编号 domain_idd1gdtb1
类 Class classa — All alpha proteins
折叠类型 Fold folda.4 — DNA/RNA-binding 3-helical bundle
超家族 Superfamily superfamilya.4.1 — Homeodomain-like
家族 Family familya.4.1.2 — Recombinase DNA-binding domain
结构域编号 domain_idd1gdtb2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.53 — Resolvase-like
超家族 Superfamily superfamilyc.53.1 — Resolvase-like
家族 Family familyc.53.1.1 — gamma,delta resolvase, catalytic domain

CATH v4.4 (6 domains)

结构域编号 domain_id1gdtA01
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily1390 — Resolvase, N-terminal catalytic domain
结构域编号 domain_id1gdtA02
类 Class class6 — Special
架构 Architecture architecture10 — Helix non-globular
拓扑 Topology topology250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id1gdtA03
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily60 — Homeodomain-like
结构域编号 domain_id1gdtB01
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily1390 — Resolvase, N-terminal catalytic domain
结构域编号 domain_id1gdtB02
类 Class class6 — Special
架构 Architecture architecture10 — Helix non-globular
拓扑 Topology topology250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id1gdtB03
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily60 — Homeodomain-like

7. 引用文献 (1)

8. 文件与曲线 (10)