1h0m

Three-dimensional structure of the quorum sensing protein TraR bound to its autoinducer and to its target DNA

Method: X-RAY DIFFRACTION

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

Transcriptional activator protein TraR

Rhizobium radiobacter

当前结构中的状态

Assembly	物理组成	蛋白状态	分子拷贝数	共同组分	数据核对
1	蛋白–DNA	同源多聚体	蛋白 2 DNA 2	;5'-D(APTPGPTPGPCPAPGPAPTP CPTPGPCPAPCPAPT)-3' ; × 2 3-OXO-OCTANOIC ACID (2-OXO-TETRAHYDRO-FURAN-3-YL)-AMIDE × 2 water × 3	与全部聚合物一致
2	蛋白–DNA	同源多聚体	蛋白 2 DNA 2	;5'-D(APTPGPTPGPCPAPGPAPTP CPTPGPCPAPCPAPT)-3' ; × 2 3-OXO-OCTANOIC ACID (2-OXO-TETRAHYDRO-FURAN-3-YL)-AMIDE × 2 water × 4	与全部聚合物一致

数据库中的同蛋白其他状态

查看构建体与数据证据

UniProt名称	TRAR_RHIRD
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 1–234； UniProt 1–234 作者链 B； PDB构建体 1–234； UniProt 1–234 作者链 C； PDB构建体 1–234； UniProt 1–234 作者链 D； PDB构建体 1–234； UniProt 1–234

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	1h0m
沉积日期 deposition_date	2002-06-25
结构标题 title	Three-dimensional structure of the quorum sensing protein TraR bound to its autoinducer and to its target DNA
关键词 keywords	;TRANSCRIPTION/DNA, LUXR-TYPE PROTEIN, QUORUM SENSING, TRANSCRIPTIONAL REGULATOR, DNA-BINDING PROTEIN, HOMOSERINE LACTONE, TRAR, TRANSCRIPTION-DNA complex ;; TRANSCRIPTION/DNA
实验方法 method	X-RAY DIFFRACTION

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3. Official assembly/model 理论 SAXS Official SAXS Profiles

页面只读取最新 assembly 计算结果。每一条曲线都对应明确的 PDB、official biological assembly 和 coordinate model。

1h0m__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

下载该曲线 .dat

1h0m__assembly_1__model_1 | I(q)

100 个绘图点；横轴和纵轴均采用对数尺度。

1h0m__assembly_1__model_1 | P(r) · Pending

P(r) 将按相同 assembly/model 分别计算和展示。

Rg（Guinier）	28.00 Å
Rg（电子密度）	26.55 Å
总 Rg	27.27 Å
原子数	4476
残基数	484
排除体积	77512 Å³
最大 q	0.500 Å⁻¹

Assembly	Model	结构单元	聚集说明	状态	CRYSOL	操作
1	1	`1h0m__assembly_1__model_1`	tetrameric （4）	成功	4.1.3-1-20251215 (887e7ef)	查看下载
2	1	`1h0m__assembly_2__model_1`	tetrameric （4）	成功	4.1.3-1-20251215 (887e7ef)	查看下载

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合物信息 Entities & Polymers (4)

实体 1 Entity 1 polymer

描述 description	Transcriptional activator protein TraR
分子量 formula_weight	27060.0 kDa
来源方法 src_method	man
拷贝数 entity_count	4

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D
原子数 atom_count	9013

MQHWLDKLTDLAAIEGDECILKTGLADIADHFGFTGYAYLHIQHRHITAVTNYHRQWQSTYFDKKFEALDPVVKRARSRKHIFTWSGEHERPTLSKDERAFYDHASDFGIRSGITIPIKTANGFMSMFTMASDKPVIDLDREIDAVAAAATIGQIHARISFLRTTPTAEDAAWLDPKEATYLRWIAVGKTMEEIADVEGVKYNSVRVKLREAMKRFDVRSKAHLTALAIRRKLI

来源 Source

来源生物 organism	Rhizobium radiobacter
通用名 common_name	Agrobacterium tumefaciens
NCBI 分类编号 ncbi_taxonomy_id	358
宿主生物 host_organism	ESCHERICHIA COLI

实体 2 Entity 2 polymer

描述 description	;5'-D(APTPGPTPGPCPAPGPAPTP CPTPGPCPAPCPAPT)-3' ;
分子量 formula_weight	5515.6 kDa
来源方法 src_method	syn
拷贝数 entity_count	4

聚合物 Polymer

聚合物类型 type	polydeoxyribonucleotide
链编号 strand_id	E,F,G,H

ATGTGCAGATCTGCACAT

实体 3 Entity 3 non-polymer

描述 description	3-OXO-OCTANOIC ACID (2-OXO-TETRAHYDRO-FURAN-3-YL)-AMIDE
分子量 formula_weight	241.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	4

实体 4 Entity 4 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	84

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6. 折叠分类 (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

结构域编号 domain_id	d1h0ma1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.4 — DNA/RNA-binding 3-helical bundle
超家族 Superfamily superfamily	a.4.6 — C-terminal effector domain of the bipartite response regulators
家族 Family family	a.4.6.2 — GerE-like (LuxR/UhpA family of transcriptional regulators)

结构域编号 domain_id	d1h0ma2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.110 — Profilin-like
超家族 Superfamily superfamily	d.110.5 — Pheromone-binding domain of LuxR-like quorum-sensing transcription factors
家族 Family family	d.110.5.1 — Pheromone-binding domain of LuxR-like quorum-sensing transcription factors

结构域编号 domain_id	d1h0mb1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.4 — DNA/RNA-binding 3-helical bundle
超家族 Superfamily superfamily	a.4.6 — C-terminal effector domain of the bipartite response regulators
家族 Family family	a.4.6.2 — GerE-like (LuxR/UhpA family of transcriptional regulators)

结构域编号 domain_id	d1h0mb2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.110 — Profilin-like
超家族 Superfamily superfamily	d.110.5 — Pheromone-binding domain of LuxR-like quorum-sensing transcription factors
家族 Family family	d.110.5.1 — Pheromone-binding domain of LuxR-like quorum-sensing transcription factors

结构域编号 domain_id	d1h0mc1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.4 — DNA/RNA-binding 3-helical bundle
超家族 Superfamily superfamily	a.4.6 — C-terminal effector domain of the bipartite response regulators
家族 Family family	a.4.6.2 — GerE-like (LuxR/UhpA family of transcriptional regulators)

结构域编号 domain_id	d1h0mc2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.110 — Profilin-like
超家族 Superfamily superfamily	d.110.5 — Pheromone-binding domain of LuxR-like quorum-sensing transcription factors
家族 Family family	d.110.5.1 — Pheromone-binding domain of LuxR-like quorum-sensing transcription factors

结构域编号 domain_id	d1h0md1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.4 — DNA/RNA-binding 3-helical bundle
超家族 Superfamily superfamily	a.4.6 — C-terminal effector domain of the bipartite response regulators
家族 Family family	a.4.6.2 — GerE-like (LuxR/UhpA family of transcriptional regulators)

结构域编号 domain_id	d1h0md2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.110 — Profilin-like
超家族 Superfamily superfamily	d.110.5 — Pheromone-binding domain of LuxR-like quorum-sensing transcription factors
家族 Family family	d.110.5.1 — Pheromone-binding domain of LuxR-like quorum-sensing transcription factors

CATH v4.4 (8 domains)

结构域编号 domain_id	1h0mA01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	450 — Beta-Lactamase
同源超家族 H-superfamily homologous superfamily	80 — Transcription factor LuxR-like, autoinducer-binding domain

结构域编号 domain_id	1h0mA02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily	10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

结构域编号 domain_id	1h0mB01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	450 — Beta-Lactamase
同源超家族 H-superfamily homologous superfamily	80 — Transcription factor LuxR-like, autoinducer-binding domain

结构域编号 domain_id	1h0mB02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily	10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

结构域编号 domain_id	1h0mC01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	450 — Beta-Lactamase
同源超家族 H-superfamily homologous superfamily	80 — Transcription factor LuxR-like, autoinducer-binding domain

结构域编号 domain_id	1h0mC02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily	10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

结构域编号 domain_id	1h0mD01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	450 — Beta-Lactamase
同源超家族 H-superfamily homologous superfamily	80 — Transcription factor LuxR-like, autoinducer-binding domain

结构域编号 domain_id	1h0mD02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily	10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

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晶胞边长 a length_a	66.993 Å
晶胞边长 b length_b	94.678 Å
晶胞边长 c length_c	209.669 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	16

空间群 space_group	P 21 21 21
空间群编号 int_tables_number	19
晶系 crystal_system	—

分辨率 resolution	3.000 Å
最低分辨率 resolution_low	20.000 Å
R 因子 r_factor	—
R_work r_work	0.2330
R_free r_free	0.2850

Three-dimensional structure of the quorum sensing protein TraR bound to its autoinducer and to its target DNA

1. 蛋白身份与相关结构 Protein Identity & Related Structures

Transcriptional activator protein TraR

当前结构中的状态

数据库中的同蛋白其他状态

2. 结构基本信息 2. Structure Basics

3. Official assembly/model 理论 SAXS Official SAXS Profiles

1h0m__assembly_1__model_1

1h0m__assembly_1__model_1 | I(q)

1h0m__assembly_1__model_1 | P(r) · Pending

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合物信息 Entities & Polymers (4)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 polymer

聚合物 Polymer

实体 3 Entity 3 non-polymer

实体 4 Entity 4 water

6. 折叠分类 (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

CATH v4.4 (8 domains)

7. 引用文献 (2)

Three-dimensional structure of the quorum sensing protein TraR bound to its autoinducer and to its target DNA

1. 蛋白身份与相关结构 Protein Identity & Related Structures

Transcriptional activator protein TraR

当前结构中的状态

数据库中的同蛋白其他状态

单体

同源二聚体

同源四聚体

其他同源多聚体

异源或核酸复合物

2. 结构基本信息 2. Structure Basics

3. Official assembly/model 理论 SAXS Official SAXS Profiles

1h0m__assembly_1__model_1

1h0m__assembly_1__model_1 | I(q)

1h0m__assembly_1__model_1 | P(r) · Pending

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合物信息 Entities & Polymers (4)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 polymer

聚合物 Polymer

实体 3 Entity 3 non-polymer

实体 4 Entity 4 water

6. 折叠分类 (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

CATH v4.4 (8 domains)

7. 引用文献 (2)