1hsk

CRYSTAL STRUCTURE OF S. AUREUS MURB

Method: X-RAY DIFFRACTION Dmax: 65.4 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 1hsk

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1hsk
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1hsk
沉积日期 deposition_date2000-12-27
结构标题 titleCRYSTAL STRUCTURE OF S. AUREUS MURB
关键词 keywordsPEPTIDOGLYCAN SYNTHESIS, CELL WALL, CELL DIVISION, OXIDOREDUCTASE, NADP, FLAVOPROTEIN, FAD; OXIDOREDUCTASE
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier20.87
回转半径 Rg (电子) rg_electron20.07
零角强度 I(0) i020501700.00
分子量 molecular_weight34097.0 kDa
排除体积 excluded_volume42560 ų
包络体积 envelope_volume49615 ų
水化壳体积 shell_volume20737 ų
包络直径 envelope_diameter71.0
壳层 Rg shell_rg26.40
包络 Rg envelope_rg20.26
形状 Rg shape_rg20.07
总 Rg total_rg20.90
总原子数 total_atoms2398
残基数 n_residues303
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax65.4
Rg (实空间) rg_real20.78
Rg 误差 (实空间) rg_real_error0.43
I(0) (实空间) i0_real2.0500e+07
I(0) 误差 (实空间) i0_real_error2.9400e+05
Rg (倒空间) rg_reciprocal20.80
I(0) (倒空间) i0_reciprocal20500000.0000
解质量估计 total_estimate0.8277
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary24.6
偏度 Skewness skewness0.204
峰度 Kurtosis kurtosis-0.499
角度范围 angular_range— – 0.3800 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha4847000.0000
实空间数据点数 n_real_points70
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.922; Stabil: 0.997; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.000

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (3)

6. 折叠分类 (SCOP + CATH) 5 domains

SCOP 2.08 (2 domains)

结构域编号 domain_idd1hska1
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.145 — FAD-binding/transporter-associated domain-like
超家族 Superfamily superfamilyd.145.1 — FAD-binding/transporter-associated domain-like
家族 Family familyd.145.1.2 — Uridine diphospho-N-Acetylenolpyruvylglucosamine reductase (MurB), N-terminal domain
结构域编号 domain_idd1hska2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.146 — Uridine diphospho-N-Acetylenolpyruvylglucosamine reductase, MurB, C-terminal domain
超家族 Superfamily superfamilyd.146.1 — Uridine diphospho-N-Acetylenolpyruvylglucosamine reductase, MurB, C-terminal domain
家族 Family familyd.146.1.1 — Uridine diphospho-N-Acetylenolpyruvylglucosamine reductase, MurB, C-terminal domain

CATH v4.4 (3 domains)

结构域编号 domain_id1hskA01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology465 — Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase; domain 3
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id1hskA02
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology43 — Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase; domain 2
同源超家族 H-superfamily homologous superfamily10 — Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2
结构域编号 domain_id1hskA03
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology78 — Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase; domain 1
同源超家族 H-superfamily homologous superfamily10 — UDP-N-acetylenolpyruvoylglucosamine reductase, C-terminal domain

7. 引用文献 (1)

8. 文件与曲线 (10)