1i5l

CRYSTAL STRUCTURE OF AN SM-LIKE PROTEIN (AF-SM1) FROM ARCHAEOGLOBUS FULGIDUS COMPLEXED WITH SHORT POLY-U RNA

Method: X-RAY DIFFRACTION D_max: 112.3 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	1i5l
沉积日期 deposition_date	2001-02-28
结构标题 title	CRYSTAL STRUCTURE OF AN SM-LIKE PROTEIN (AF-SM1) FROM ARCHAEOGLOBUS FULGIDUS COMPLEXED WITH SHORT POLY-U RNA
关键词 keywords	SNRNP, SM, CORE SNRNP DOMAIN, RNA BINDING PROTEIN, SINGLE-STRANDED RNA BINDING PROTEIN, RNA BINDING PROTEIN-RNA COMPLEX; RNA BINDING PROTEIN/RNA
实验方法 method	X-RAY DIFFRACTION

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	34.55 Å
回转半径 Rg (电子) rg_electron	34.20 Å
零角强度 I(0) i0	196319000.00
分子量 molecular_weight	113420.0 kDa
排除体积 excluded_volume	143000 Å³
包络体积 envelope_volume	189840 Å³
水化壳体积 shell_volume	46674 Å³
包络直径 envelope_diameter	116.2 Å
壳层 Rg shell_rg	40.52 Å
包络 Rg envelope_rg	33.64 Å
形状 Rg shape_rg	34.20 Å
总 Rg total_rg	34.68 Å
总原子数 total_atoms	7979
残基数 n_residues	1016
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	112.3 Å
Rg (实空间) rg_real	34.55 Å
Rg 误差 (实空间) rg_real_error	0.70 Å
I(0) (实空间) i0_real	1.9630e+08
I(0) 误差 (实空间) i0_real_error	3.1040e+06
Rg (倒空间) rg_reciprocal	34.55 Å
I(0) (倒空间) i0_reciprocal	196300000.0000
解质量估计 total_estimate	0.8821
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	43.9 Å
偏度 Skewness skewness	0.350
峰度 Kurtosis kurtosis	-0.368
角度范围 angular_range	— – 0.2300 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	134900000.0000
实空间数据点数 n_real_points	47
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.882; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.819

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (4)

Entity 1 polymer

描述 description	5'-R(UPUP*U)-3'
分子量 formula_weight	873.5 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	U,Y
原子数 atom_count	8036

UUU

Entity 2 polymer

描述 description	PUTATIVE SNRNP SM-LIKE PROTEIN AF-SM1
分子量 formula_weight	8391.7 kDa
来源方法 src_method	man
拷贝数 entity_count	14

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D,E,F,G,H,I,J,K,L,M,N

MPPRPLDVLNRSLKSPVIVRLKGGREFRGTLDGYDIHMNLVLLDAEEIQNGEVVRKVGSVVIRGDTVVFVSPAPGGE

来源 Source

来源生物 organism	Archaeoglobus fulgidus
NCBI 分类编号 ncbi_taxonomy_id	2234
宿主生物 host_organism	Escherichia coli BL21(DE3)

Entity 3 non-polymer

描述 description	URIDINE
分子量 formula_weight	244.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	4

Entity 4 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	57

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6. 折叠分类 (SCOP + CATH) 28 domains

SCOP 2.08 (14 domains)

结构域编号 domain_id	d1i5la_
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.38 — Sm-like fold
超家族 Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
家族 Family family	b.38.1.1 — Sm motif of small nuclear ribonucleoproteins, SNRNP

结构域编号 domain_id	d1i5lb_
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.38 — Sm-like fold
超家族 Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
家族 Family family	b.38.1.1 — Sm motif of small nuclear ribonucleoproteins, SNRNP

结构域编号 domain_id	d1i5lc_
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.38 — Sm-like fold
超家族 Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
家族 Family family	b.38.1.1 — Sm motif of small nuclear ribonucleoproteins, SNRNP

结构域编号 domain_id	d1i5ld_
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.38 — Sm-like fold
超家族 Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
家族 Family family	b.38.1.1 — Sm motif of small nuclear ribonucleoproteins, SNRNP

结构域编号 domain_id	d1i5le_
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.38 — Sm-like fold
超家族 Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
家族 Family family	b.38.1.1 — Sm motif of small nuclear ribonucleoproteins, SNRNP

结构域编号 domain_id	d1i5lf_
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.38 — Sm-like fold
超家族 Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
家族 Family family	b.38.1.1 — Sm motif of small nuclear ribonucleoproteins, SNRNP

结构域编号 domain_id	d1i5lg_
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.38 — Sm-like fold
超家族 Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
家族 Family family	b.38.1.1 — Sm motif of small nuclear ribonucleoproteins, SNRNP

结构域编号 domain_id	d1i5lh_
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.38 — Sm-like fold
超家族 Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
家族 Family family	b.38.1.1 — Sm motif of small nuclear ribonucleoproteins, SNRNP

结构域编号 domain_id	d1i5li_
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.38 — Sm-like fold
超家族 Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
家族 Family family	b.38.1.1 — Sm motif of small nuclear ribonucleoproteins, SNRNP

结构域编号 domain_id	d1i5lj_
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.38 — Sm-like fold
超家族 Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
家族 Family family	b.38.1.1 — Sm motif of small nuclear ribonucleoproteins, SNRNP

结构域编号 domain_id	d1i5lk_
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.38 — Sm-like fold
超家族 Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
家族 Family family	b.38.1.1 — Sm motif of small nuclear ribonucleoproteins, SNRNP

结构域编号 domain_id	d1i5ll_
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.38 — Sm-like fold
超家族 Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
家族 Family family	b.38.1.1 — Sm motif of small nuclear ribonucleoproteins, SNRNP

结构域编号 domain_id	d1i5lm_
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.38 — Sm-like fold
超家族 Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
家族 Family family	b.38.1.1 — Sm motif of small nuclear ribonucleoproteins, SNRNP

结构域编号 domain_id	d1i5ln_
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.38 — Sm-like fold
超家族 Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
家族 Family family	b.38.1.1 — Sm motif of small nuclear ribonucleoproteins, SNRNP

CATH v4.4 (14 domains)

结构域编号 domain_id	1i5lA00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily	100 —

结构域编号 domain_id	1i5lB00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily	100 —

结构域编号 domain_id	1i5lC00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily	100 —

结构域编号 domain_id	1i5lD00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily	100 —

结构域编号 domain_id	1i5lE00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily	100 —

结构域编号 domain_id	1i5lF00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily	100 —

结构域编号 domain_id	1i5lG00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily	100 —

结构域编号 domain_id	1i5lH00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily	100 —

结构域编号 domain_id	1i5lI00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily	100 —

结构域编号 domain_id	1i5lJ00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily	100 —

结构域编号 domain_id	1i5lK00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily	100 —

结构域编号 domain_id	1i5lL00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily	100 —

结构域编号 domain_id	1i5lM00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily	100 —

结构域编号 domain_id	1i5lN00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily	100 —

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7. 引用文献 (1)

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晶胞边长 a length_a	69.858 Å
晶胞边长 b length_b	130.438 Å
晶胞边长 c length_c	70.047 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	115.36 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	28

空间群 space_group	P 1 21 1
空间群编号 int_tables_number	4
晶系 crystal_system	—

分辨率 resolution	2.750 Å
最低分辨率 resolution_low	25.000 Å
R 因子 r_factor	0.2290
R_work r_work	0.2250
R_free r_free	0.3000

fit	/app/data/saxs_computation/i5/1i5l/1i5l.fit
pddf	/app/data/pr_computation/i5/1i5l/1i5l.pr.out
profile	/app/data/saxs_computation/i5/1i5l/1i5l.dat

CRYSTAL STRUCTURE OF AN SM-LIKE PROTEIN (AF-SM1) FROM ARCHAEOGLOBUS FULGIDUS COMPLEXED WITH SHORT POLY-U RNA

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合体信息 (4)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

来源 Source

Entity 3 non-polymer

Entity 4 water

6. 折叠分类 (SCOP + CATH) 28 domains

SCOP 2.08 (14 domains)

CATH v4.4 (14 domains)

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)