1j1z

Crystal Structure of Thermus thermophilus HB8 Argininosuccinate Synthetase in complex with substrate

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Argininosuccinate Synthetase

Thermus thermophilus

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 4	ADENOSINE-5'-TRIPHOSPHATE × 4 CITRULLINE × 4 ASPARTIC ACID × 4 water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	ASSY_THET8
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–400； UniProt 1–400 Author chain B； PDBConstruct 1–400； UniProt 1–400 Author chain C； PDBConstruct 1–400； UniProt 1–400 Author chain D； PDBConstruct 1–400； UniProt 1–400

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	1j1z
Deposition date deposition_date	2002-12-24
Structure title title	Crystal Structure of Thermus thermophilus HB8 Argininosuccinate Synthetase in complex with substrate
Keywords keywords	LIGASE, ATP-binding, RIKEN Structural Genomics/Proteomics Initiative, RSGI, Structural Genomics; LIGASE
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1j1z__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1j1z__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

1j1z__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	34.82 Å
Rg (electron density)	33.95 Å
Total Rg	34.62 Å
Atom count	12472
Residues	1544
Excluded volume	221560 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`1j1z__assembly_1__model_1`	tetrameric （4）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Description description	Argininosuccinate Synthetase
Molecular weight formula_weight	44876.0 kDa
EC 编号 ec_number	6.3.4.5
Source method src_method	man
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D
Atom count atom_count	13150

MKIVLAYSGGLDTSIILKWLKETYRAEVIAFTADIGQGEEVEEAREKALRTGASKAIALDLKEEFVRDFVFPMMRAGAVYEGYYLLGTSIARPLIAKHLVRIAEEEGAEAIAHGATGKGNDQVRFELTAYALKPDIKVIAPWREWSFQGRKEMIAYAEAHGIPVPVTQEKPYSMDANLLHISYEGGVLEDPWAEPPKGMFRMTQDPEEAPDAPEYVEVEFFEGDPVAVNGERLSPAALLQRLNEIGGRHGVGRVDIVENRFVGMKSRGVYETPGGTILYHARRAVESLTLDREVLHQRDMLSPKYAELVYYGFWYAPEREALQAYFDHVARSVTGVARLKLYKGNVYVVGRKAPKSLYRQDLVSFDEAGGYDQKDAEGFIKIQALRLRVRALVEREGHGA

Source Source

Source organism organism	Thermus thermophilus
NCBI taxonomy ID ncbi_taxonomy_id	274
Host organism host_organism	Escherichia coli

Entity 2 Entity 2 non-polymer

Description description	ADENOSINE-5'-TRIPHOSPHATE
Molecular weight formula_weight	507.2 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 3 Entity 3 non-polymer

Description description	CITRULLINE
Molecular weight formula_weight	175.2 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 4 Entity 4 non-polymer

Description description	ASPARTIC ACID
Molecular weight formula_weight	133.1 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 5 Entity 5 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	678

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6. Fold Classification (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

Domain ID domain_id	d1j1za1
Class class	c — Alpha and beta proteins (a/b)
Fold Fold fold	c.26 — Adenine nucleotide alpha hydrolase-like
Superfamily Superfamily superfamily	c.26.2 — Adenine nucleotide alpha hydrolases-like
Family Family family	c.26.2.1 — N-type ATP pyrophosphatases

Domain ID domain_id	d1j1za2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.210 — Argininosuccinate synthetase, C-terminal domain
Superfamily Superfamily superfamily	d.210.1 — Argininosuccinate synthetase, C-terminal domain
Family Family family	d.210.1.1 — Argininosuccinate synthetase, C-terminal domain

Domain ID domain_id	d1j1zb1
Class class	c — Alpha and beta proteins (a/b)
Fold Fold fold	c.26 — Adenine nucleotide alpha hydrolase-like
Superfamily Superfamily superfamily	c.26.2 — Adenine nucleotide alpha hydrolases-like
Family Family family	c.26.2.1 — N-type ATP pyrophosphatases

Domain ID domain_id	d1j1zb2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.210 — Argininosuccinate synthetase, C-terminal domain
Superfamily Superfamily superfamily	d.210.1 — Argininosuccinate synthetase, C-terminal domain
Family Family family	d.210.1.1 — Argininosuccinate synthetase, C-terminal domain

Domain ID domain_id	d1j1zc1
Class class	c — Alpha and beta proteins (a/b)
Fold Fold fold	c.26 — Adenine nucleotide alpha hydrolase-like
Superfamily Superfamily superfamily	c.26.2 — Adenine nucleotide alpha hydrolases-like
Family Family family	c.26.2.1 — N-type ATP pyrophosphatases

Domain ID domain_id	d1j1zc2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.210 — Argininosuccinate synthetase, C-terminal domain
Superfamily Superfamily superfamily	d.210.1 — Argininosuccinate synthetase, C-terminal domain
Family Family family	d.210.1.1 — Argininosuccinate synthetase, C-terminal domain

Domain ID domain_id	d1j1zd1
Class class	c — Alpha and beta proteins (a/b)
Fold Fold fold	c.26 — Adenine nucleotide alpha hydrolase-like
Superfamily Superfamily superfamily	c.26.2 — Adenine nucleotide alpha hydrolases-like
Family Family family	c.26.2.1 — N-type ATP pyrophosphatases

Domain ID domain_id	d1j1zd2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.210 — Argininosuccinate synthetase, C-terminal domain
Superfamily Superfamily superfamily	d.210.1 — Argininosuccinate synthetase, C-terminal domain
Family Family family	d.210.1.1 — Argininosuccinate synthetase, C-terminal domain

CATH v4.4 (8 domains)

Domain ID domain_id	1j1zA01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	50 — Rossmann fold
Homologous superfamily homologous superfamily	620 — HUPs

Domain ID domain_id	1j1zA02
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	1260 — Argininosuccinate synthetase, chain A, domain 2
Homologous superfamily homologous superfamily	10 — Argininosuccinate synthetase, chain A, domain 2

Domain ID domain_id	1j1zB01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	50 — Rossmann fold
Homologous superfamily homologous superfamily	620 — HUPs

Domain ID domain_id	1j1zB02
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	1260 — Argininosuccinate synthetase, chain A, domain 2
Homologous superfamily homologous superfamily	10 — Argininosuccinate synthetase, chain A, domain 2

Domain ID domain_id	1j1zC01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	50 — Rossmann fold
Homologous superfamily homologous superfamily	620 — HUPs

Domain ID domain_id	1j1zC02
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	1260 — Argininosuccinate synthetase, chain A, domain 2
Homologous superfamily homologous superfamily	10 — Argininosuccinate synthetase, chain A, domain 2

Domain ID domain_id	1j1zD01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	50 — Rossmann fold
Homologous superfamily homologous superfamily	620 — HUPs

Domain ID domain_id	1j1zD02
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	1260 — Argininosuccinate synthetase, chain A, domain 2
Homologous superfamily homologous superfamily	10 — Argininosuccinate synthetase, chain A, domain 2

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Unit-cell length a length_a	229.330 Å
Unit-cell length b length_b	229.330 Å
Unit-cell length c length_c	159.260 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	120.00 °
Molecules per unit cell Z z_pdb	36

Space group space_group	H 3
Space-group number int_tables_number	146
Crystal system crystal_system	—

Resolution resolution	2.100 Å
Low-resolution limit resolution_low	10.000 Å
R factor r_factor	—
R_work r_work	0.2260
R_free r_free	0.2570

Crystal Structure of Thermus thermophilus HB8 Argininosuccinate Synthetase in complex with substrate

1. Protein Identity and Related Structures Protein Identity & Related Structures

Argininosuccinate Synthetase

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1j1z__assembly_1__model_1

1j1z__assembly_1__model_1 | I(q)

1j1z__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 water

6. Fold Classification (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

CATH v4.4 (8 domains)

7. Citations (1)

Crystal Structure of Thermus thermophilus HB8 Argininosuccinate Synthetase in complex with substrate

1. Protein Identity and Related Structures Protein Identity & Related Structures

Argininosuccinate Synthetase

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1j1z__assembly_1__model_1

1j1z__assembly_1__model_1 | I(q)

1j1z__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 water

6. Fold Classification (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

CATH v4.4 (8 domains)

7. Citations (1)