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1j3e

Crystal Structure of the E.coli SeqA protein complexed with N6-methyladenine- guanine mismatch DNA

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

SeqA protein

Escherichia coli

UniProt P36658

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Monomer Protein 1 DNA 2 5'-D(*AP*AP*GP*GP*(6MA)P*TP*CP*CP*AP*A)-3' × 1 5'-D(*TP*TP*GP*GP*AP*GP*CP*CP*TP*T)-3' × 1 water × 3 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer
1xrx 1xrx

2 assemblies

Homotetramer

None

Other homooligomers
1xrx

1 assemblies

Heteromeric or nucleic-acid complex
1iu3 1j3e 1lrr 1lrr

4 assemblies

View Construct and Data Evidence
UniProt name SEQA_ECOLI
Isoform —
PDB entities 3
Chains and sequence ranges Author chain A； PDBConstruct 5–115； UniProt 71–181

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id1j3e
Deposition date deposition_date2003-01-24
Structure title titleCrystal Structure of the E.coli SeqA protein complexed with N6-methyladenine- guanine mismatch DNA
Keywords keywords;Protein-DNA complex, Recognition of hemimethylated DNA, mismatched DNA, replication, RIKEN Structural Genomics/Proteomics Initiative, RSGI, Structural Genomics, REPLICATION-DNA COMPLEX ;; REPLICATION/DNA
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1j3e__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1j3e__assembly_1__model_1 | I(q)

10-2 10-1 105 106 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

1j3e__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）16.69 Å
Rg (electron density)15.70 Å
Total Rg16.61 Å
Atom count1315
Residues134
Excluded volume22160 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 1j3e__assembly_1__model_1 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (4)

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6. Fold Classification (SCOP + CATH) 3 domains

SCOP 2.08 (2 domains)

Domain ID domain_idd1j3ea1
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.228 — Replication modulator SeqA, C-terminal DNA-binding domain
Superfamily Superfamily superfamilyd.228.1 — Replication modulator SeqA, C-terminal DNA-binding domain
Family Family familyd.228.1.1 — Replication modulator SeqA, C-terminal DNA-binding domain
Domain ID domain_idd1j3ea2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags

CATH v4.4 (1 domains)

Domain ID domain_id1j3eA00
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1380 — Replication modulator SeqA, C-terminal DNA-binding domain
Homologous superfamily homologous superfamily10 — Replication modulator SeqA, C-terminal DNA-binding domain
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7. Citations (1)