1kf6

E. coli Quinol-Fumarate Reductase with Bound Inhibitor HQNO

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

FUMARATE REDUCTASE FLAVOPROTEIN

Escherichia coli

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 4	FUMARATE REDUCTASE IRON-SULFUR PROTEIN × 1 （P0AC47） FUMARATE REDUCTASE 15 KDA HYDROPHOBIC PROTEIN × 1 （P0A8Q0） FUMARATE REDUCTASE 13 KDA HYDROPHOBIC PROTEIN × 1 （P0A8Q3） POTASSIUM ION × 1 OXALOACETATE ION × 1 ACETATE ION × 2 FLAVIN-ADENINE DINUCLEOTIDE × 1 PENTAETHYLENE GLYCOL × 1 FE2/S2 (INORGANIC) CLUSTER × 1 FE3-S4 CLUSTER × 1 IRON/SULFUR CLUSTER × 1 2-HEPTYL-4-HYDROXY QUINOLINE N-OXIDE × 1 water × 2	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 4	FUMARATE REDUCTASE IRON-SULFUR PROTEIN × 1 （P0AC47） FUMARATE REDUCTASE 15 KDA HYDROPHOBIC PROTEIN × 1 （P0A8Q0） FUMARATE REDUCTASE 13 KDA HYDROPHOBIC PROTEIN × 1 （P0A8Q3） POTASSIUM ION × 1 OXALOACETATE ION × 1 ACETATE ION × 1 FLAVIN-ADENINE DINUCLEOTIDE × 1 FE2/S2 (INORGANIC) CLUSTER × 1 FE3-S4 CLUSTER × 1 IRON/SULFUR CLUSTER × 1 2-HEPTYL-4-HYDROXY QUINOLINE N-OXIDE × 1 O-DODECANYL OCTAETHYLENE GLYCOL × 5 water × 2	Consistent with protein count
3	Protein heterocomplex	Heteromer	Protein 8	FUMARATE REDUCTASE IRON-SULFUR PROTEIN × 2 （P0AC47） FUMARATE REDUCTASE 15 KDA HYDROPHOBIC PROTEIN × 2 （P0A8Q0） FUMARATE REDUCTASE 13 KDA HYDROPHOBIC PROTEIN × 2 （P0A8Q3） POTASSIUM ION × 2 OXALOACETATE ION × 2 ACETATE ION × 3 FLAVIN-ADENINE DINUCLEOTIDE × 2 PENTAETHYLENE GLYCOL × 1 FE2/S2 (INORGANIC) CLUSTER × 2 FE3-S4 CLUSTER × 2 IRON/SULFUR CLUSTER × 2 2-HEPTYL-4-HYDROXY QUINOLINE N-OXIDE × 2 O-DODECANYL OCTAETHYLENE GLYCOL × 5 water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	FRDA_ECOLI
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–602； UniProt 0–601 Author chain M； PDBConstruct 1–602； UniProt 0–601

FUMARATE REDUCTASE IRON-SULFUR PROTEIN

Escherichia coli

UniProt P0AC47

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 4	FUMARATE REDUCTASE FLAVOPROTEIN × 1 （P00363） FUMARATE REDUCTASE 15 KDA HYDROPHOBIC PROTEIN × 1 （P0A8Q0） FUMARATE REDUCTASE 13 KDA HYDROPHOBIC PROTEIN × 1 （P0A8Q3） POTASSIUM ION × 1 OXALOACETATE ION × 1 ACETATE ION × 2 FLAVIN-ADENINE DINUCLEOTIDE × 1 PENTAETHYLENE GLYCOL × 1 FE2/S2 (INORGANIC) CLUSTER × 1 FE3-S4 CLUSTER × 1 IRON/SULFUR CLUSTER × 1 2-HEPTYL-4-HYDROXY QUINOLINE N-OXIDE × 1 water × 2	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 4	FUMARATE REDUCTASE FLAVOPROTEIN × 1 （P00363） FUMARATE REDUCTASE 15 KDA HYDROPHOBIC PROTEIN × 1 （P0A8Q0） FUMARATE REDUCTASE 13 KDA HYDROPHOBIC PROTEIN × 1 （P0A8Q3） POTASSIUM ION × 1 OXALOACETATE ION × 1 ACETATE ION × 1 FLAVIN-ADENINE DINUCLEOTIDE × 1 FE2/S2 (INORGANIC) CLUSTER × 1 FE3-S4 CLUSTER × 1 IRON/SULFUR CLUSTER × 1 2-HEPTYL-4-HYDROXY QUINOLINE N-OXIDE × 1 O-DODECANYL OCTAETHYLENE GLYCOL × 5 water × 2	Consistent with protein count
3	Protein heterocomplex	Heteromer	Protein 8	FUMARATE REDUCTASE FLAVOPROTEIN × 2 （P00363） FUMARATE REDUCTASE 15 KDA HYDROPHOBIC PROTEIN × 2 （P0A8Q0） FUMARATE REDUCTASE 13 KDA HYDROPHOBIC PROTEIN × 2 （P0A8Q3） POTASSIUM ION × 2 OXALOACETATE ION × 2 ACETATE ION × 3 FLAVIN-ADENINE DINUCLEOTIDE × 2 PENTAETHYLENE GLYCOL × 1 FE2/S2 (INORGANIC) CLUSTER × 2 FE3-S4 CLUSTER × 2 IRON/SULFUR CLUSTER × 2 2-HEPTYL-4-HYDROXY QUINOLINE N-OXIDE × 2 O-DODECANYL OCTAETHYLENE GLYCOL × 5 water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	FRDB_ECOLI
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–243； UniProt 1–243 Author chain N； PDBConstruct 1–243； UniProt 1–243

FUMARATE REDUCTASE 15 KDA HYDROPHOBIC PROTEIN

Escherichia coli

UniProt P0A8Q0

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 4	FUMARATE REDUCTASE FLAVOPROTEIN × 1 （P00363） FUMARATE REDUCTASE IRON-SULFUR PROTEIN × 1 （P0AC47） FUMARATE REDUCTASE 13 KDA HYDROPHOBIC PROTEIN × 1 （P0A8Q3） POTASSIUM ION × 1 OXALOACETATE ION × 1 ACETATE ION × 2 FLAVIN-ADENINE DINUCLEOTIDE × 1 PENTAETHYLENE GLYCOL × 1 FE2/S2 (INORGANIC) CLUSTER × 1 FE3-S4 CLUSTER × 1 IRON/SULFUR CLUSTER × 1 2-HEPTYL-4-HYDROXY QUINOLINE N-OXIDE × 1 water × 2	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 4	FUMARATE REDUCTASE FLAVOPROTEIN × 1 （P00363） FUMARATE REDUCTASE IRON-SULFUR PROTEIN × 1 （P0AC47） FUMARATE REDUCTASE 13 KDA HYDROPHOBIC PROTEIN × 1 （P0A8Q3） POTASSIUM ION × 1 OXALOACETATE ION × 1 ACETATE ION × 1 FLAVIN-ADENINE DINUCLEOTIDE × 1 FE2/S2 (INORGANIC) CLUSTER × 1 FE3-S4 CLUSTER × 1 IRON/SULFUR CLUSTER × 1 2-HEPTYL-4-HYDROXY QUINOLINE N-OXIDE × 1 O-DODECANYL OCTAETHYLENE GLYCOL × 5 water × 2	Consistent with protein count
3	Protein heterocomplex	Heteromer	Protein 8	FUMARATE REDUCTASE FLAVOPROTEIN × 2 （P00363） FUMARATE REDUCTASE IRON-SULFUR PROTEIN × 2 （P0AC47） FUMARATE REDUCTASE 13 KDA HYDROPHOBIC PROTEIN × 2 （P0A8Q3） POTASSIUM ION × 2 OXALOACETATE ION × 2 ACETATE ION × 3 FLAVIN-ADENINE DINUCLEOTIDE × 2 PENTAETHYLENE GLYCOL × 1 FE2/S2 (INORGANIC) CLUSTER × 2 FE3-S4 CLUSTER × 2 IRON/SULFUR CLUSTER × 2 2-HEPTYL-4-HYDROXY QUINOLINE N-OXIDE × 2 O-DODECANYL OCTAETHYLENE GLYCOL × 5 water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	FRDC_ECOLI
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain C； PDBConstruct 1–130； UniProt 2–131 Author chain O； PDBConstruct 1–130； UniProt 2–131

FUMARATE REDUCTASE 13 KDA HYDROPHOBIC PROTEIN

Escherichia coli

UniProt P0A8Q3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 4	FUMARATE REDUCTASE FLAVOPROTEIN × 1 （P00363） FUMARATE REDUCTASE IRON-SULFUR PROTEIN × 1 （P0AC47） FUMARATE REDUCTASE 15 KDA HYDROPHOBIC PROTEIN × 1 （P0A8Q0） POTASSIUM ION × 1 OXALOACETATE ION × 1 ACETATE ION × 2 FLAVIN-ADENINE DINUCLEOTIDE × 1 PENTAETHYLENE GLYCOL × 1 FE2/S2 (INORGANIC) CLUSTER × 1 FE3-S4 CLUSTER × 1 IRON/SULFUR CLUSTER × 1 2-HEPTYL-4-HYDROXY QUINOLINE N-OXIDE × 1 water × 2	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 4	FUMARATE REDUCTASE FLAVOPROTEIN × 1 （P00363） FUMARATE REDUCTASE IRON-SULFUR PROTEIN × 1 （P0AC47） FUMARATE REDUCTASE 15 KDA HYDROPHOBIC PROTEIN × 1 （P0A8Q0） POTASSIUM ION × 1 OXALOACETATE ION × 1 ACETATE ION × 1 FLAVIN-ADENINE DINUCLEOTIDE × 1 FE2/S2 (INORGANIC) CLUSTER × 1 FE3-S4 CLUSTER × 1 IRON/SULFUR CLUSTER × 1 2-HEPTYL-4-HYDROXY QUINOLINE N-OXIDE × 1 O-DODECANYL OCTAETHYLENE GLYCOL × 5 water × 2	Consistent with protein count
3	Protein heterocomplex	Heteromer	Protein 8	FUMARATE REDUCTASE FLAVOPROTEIN × 2 （P00363） FUMARATE REDUCTASE IRON-SULFUR PROTEIN × 2 （P0AC47） FUMARATE REDUCTASE 15 KDA HYDROPHOBIC PROTEIN × 2 （P0A8Q0） POTASSIUM ION × 2 OXALOACETATE ION × 2 ACETATE ION × 3 FLAVIN-ADENINE DINUCLEOTIDE × 2 PENTAETHYLENE GLYCOL × 1 FE2/S2 (INORGANIC) CLUSTER × 2 FE3-S4 CLUSTER × 2 IRON/SULFUR CLUSTER × 2 2-HEPTYL-4-HYDROXY QUINOLINE N-OXIDE × 2 O-DODECANYL OCTAETHYLENE GLYCOL × 5 water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	FRDD_ECOLI
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain D； PDBConstruct 1–119； UniProt 1–119 Author chain P； PDBConstruct 1–119； UniProt 1–119

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	1kf6
Deposition date deposition_date	2001-11-19
Structure title title	E. coli Quinol-Fumarate Reductase with Bound Inhibitor HQNO
Keywords keywords	respiration, fumarate reductace, succinate dehydrogenase, complex II, quinol, quinone, OXIDOREDUCTASE; OXIDOREDUCTASE
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1kf6__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1kf6__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

1kf6__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	32.36 Å
Rg (electron density)	33.01 Å
Total Rg	33.10 Å
Atom count	8445
Residues	1069
Excluded volume	150900 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`1kf6__assembly_1__model_1`	tetrameric （4）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`1kf6__assembly_2__model_1`	tetrameric （4）	Success	4.1.3-1-20251215 (887e7ef)	View Download
3	1	`1kf6__assembly_3__model_1`	octameric （8）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (15)

Entity 1 Entity 1 polymer

Description description	FUMARATE REDUCTASE FLAVOPROTEIN
Molecular weight formula_weight	66057.6 kDa
EC 编号 ec_number	1.3.99.1
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,M
Atom count atom_count	17071

MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRSHTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGCPWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLDILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMALSHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLGEPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICELAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRLGSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWAKIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIELGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEYSDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 2 Entity 2 polymer

Description description	FUMARATE REDUCTASE IRON-SULFUR PROTEIN
Molecular weight formula_weight	27021.9 kDa
EC 编号 ec_number	1.3.99.1
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B,N

AEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYIKDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERDLVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACPQFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKHVDPAAAIQQGKVESSKDFLIATLKPR

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 3 Entity 3 polymer

Description description	FUMARATE REDUCTASE 15 KDA HYDROPHOBIC PROTEIN
Molecular weight formula_weight	14898.8 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C,O

TTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELIFGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDEKMGPEPIIKSLWAVTVVATIVILFVALYW

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 4 Entity 4 polymer

Description description	FUMARATE REDUCTASE 13 KDA HYDROPHOBIC PROTEIN
Molecular weight formula_weight	13118.9 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D,P

MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGLFPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFYGLAAILTVVTLIGVVTI

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 5 Entity 5 non-polymer

Description description	POTASSIUM ION
Molecular weight formula_weight	39.1 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 6 Entity 6 non-polymer

Description description	OXALOACETATE ION
Molecular weight formula_weight	131.1 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 7 Entity 7 non-polymer

Description description	ACETATE ION
Molecular weight formula_weight	59.0 kDa
Source method src_method	syn
Copy count entity_count	3

Entity 8 Entity 8 non-polymer

Description description	FLAVIN-ADENINE DINUCLEOTIDE
Molecular weight formula_weight	785.5 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 9 Entity 9 non-polymer

Description description	PENTAETHYLENE GLYCOL
Molecular weight formula_weight	238.3 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 10 Entity 10 non-polymer

Description description	FE2/S2 (INORGANIC) CLUSTER
Molecular weight formula_weight	175.8 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 11 Entity 11 non-polymer

Description description	FE3-S4 CLUSTER
Molecular weight formula_weight	295.8 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 12 Entity 12 non-polymer

Description description	IRON/SULFUR CLUSTER
Molecular weight formula_weight	351.6 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 13 Entity 13 non-polymer

Description description	2-HEPTYL-4-HYDROXY QUINOLINE N-OXIDE
Molecular weight formula_weight	259.3 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 14 Entity 14 non-polymer

Description description	O-DODECANYL OCTAETHYLENE GLYCOL
Molecular weight formula_weight	538.8 kDa
Source method src_method	syn
Copy count entity_count	5

Entity 15 Entity 15 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	16

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6. Fold Classification (SCOP + CATH) 30 domains

SCOP 2.08 (14 domains)

Domain ID domain_id	d1kf6a1
Class class	a — All alpha proteins
Fold Fold fold	a.7 — Spectrin repeat-like
Superfamily Superfamily superfamily	a.7.3 — Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain
Family Family family	a.7.3.1 — Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain

Domain ID domain_id	d1kf6a2
Class class	c — Alpha and beta proteins (a/b)
Fold Fold fold	c.3 — FAD/NAD(P)-binding domain
Superfamily Superfamily superfamily	c.3.1 — FAD/NAD(P)-binding domain
Family Family family	c.3.1.4 — Succinate dehydrogenase/fumarate reductase flavoprotein N-terminal domain

Domain ID domain_id	d1kf6a3
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.168 — Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain
Superfamily Superfamily superfamily	d.168.1 — Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain
Family Family family	d.168.1.1 — Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain

Domain ID domain_id	d1kf6b1
Class class	a — All alpha proteins
Fold Fold fold	a.1 — Globin-like
Superfamily Superfamily superfamily	a.1.2 — alpha-helical ferredoxin
Family Family family	a.1.2.1 — Fumarate reductase/Succinate dehydogenase iron-sulfur protein, C-terminal domain

Domain ID domain_id	d1kf6b2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.15 — beta-Grasp (ubiquitin-like)
Superfamily Superfamily superfamily	d.15.4 — 2Fe-2S ferredoxin-like
Family Family family	d.15.4.2 — 2Fe-2S ferredoxin domains from multidomain proteins

Domain ID domain_id	d1kf6c_
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.21 — Heme-binding four-helical bundle
Superfamily Superfamily superfamily	f.21.2 — Fumarate reductase respiratory complex transmembrane subunits
Family Family family	f.21.2.2 — Succinate dehydrogenase/Fumarate reductase transmembrane subunits (SdhC/FrdC and SdhD/FrdD)

Domain ID domain_id	d1kf6d_
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.21 — Heme-binding four-helical bundle
Superfamily Superfamily superfamily	f.21.2 — Fumarate reductase respiratory complex transmembrane subunits
Family Family family	f.21.2.2 — Succinate dehydrogenase/Fumarate reductase transmembrane subunits (SdhC/FrdC and SdhD/FrdD)

Domain ID domain_id	d1kf6m1
Class class	a — All alpha proteins
Fold Fold fold	a.7 — Spectrin repeat-like
Superfamily Superfamily superfamily	a.7.3 — Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain
Family Family family	a.7.3.1 — Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain

Domain ID domain_id	d1kf6m2
Class class	c — Alpha and beta proteins (a/b)
Fold Fold fold	c.3 — FAD/NAD(P)-binding domain
Superfamily Superfamily superfamily	c.3.1 — FAD/NAD(P)-binding domain
Family Family family	c.3.1.4 — Succinate dehydrogenase/fumarate reductase flavoprotein N-terminal domain

Domain ID domain_id	d1kf6m3
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.168 — Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain
Superfamily Superfamily superfamily	d.168.1 — Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain
Family Family family	d.168.1.1 — Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain

Domain ID domain_id	d1kf6n1
Class class	a — All alpha proteins
Fold Fold fold	a.1 — Globin-like
Superfamily Superfamily superfamily	a.1.2 — alpha-helical ferredoxin
Family Family family	a.1.2.1 — Fumarate reductase/Succinate dehydogenase iron-sulfur protein, C-terminal domain

Domain ID domain_id	d1kf6n2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.15 — beta-Grasp (ubiquitin-like)
Superfamily Superfamily superfamily	d.15.4 — 2Fe-2S ferredoxin-like
Family Family family	d.15.4.2 — 2Fe-2S ferredoxin domains from multidomain proteins

Domain ID domain_id	d1kf6o_
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.21 — Heme-binding four-helical bundle
Superfamily Superfamily superfamily	f.21.2 — Fumarate reductase respiratory complex transmembrane subunits
Family Family family	f.21.2.2 — Succinate dehydrogenase/Fumarate reductase transmembrane subunits (SdhC/FrdC and SdhD/FrdD)

Domain ID domain_id	d1kf6p_
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.21 — Heme-binding four-helical bundle
Superfamily Superfamily superfamily	f.21.2 — Fumarate reductase respiratory complex transmembrane subunits
Family Family family	f.21.2.2 — Succinate dehydrogenase/Fumarate reductase transmembrane subunits (SdhC/FrdC and SdhD/FrdD)

CATH v4.4 (16 domains)

Domain ID domain_id	1kf6A01
Class class	3 — Alpha Beta
Architecture architecture	50 — 3-Layer(bba) Sandwich
Topology topology	50 — FAD/NAD(P)-binding domain
Homologous superfamily homologous superfamily	60 — FAD/NAD(P)-binding domain

Domain ID domain_id	1kf6A02
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	700 — Flavocytochrome C3; Chain A, domain 1
Homologous superfamily homologous superfamily	10 — Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain

Domain ID domain_id	1kf6A03
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
Homologous superfamily homologous superfamily	100 — Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain

Domain ID domain_id	1kf6A04
Class class	4 — Few Secondary Structures
Architecture architecture	10 — Irregular
Topology topology	80 — Rhinovirus 14, subunit 4
Homologous superfamily homologous superfamily	40 — succinate dehydrogenase protein domain

Domain ID domain_id	1kf6B01
Class class	3 — Alpha Beta
Architecture architecture	10 — Roll
Topology topology	20 — Ubiquitin-like (UB roll)
Homologous superfamily homologous superfamily	30 — Beta-grasp domain

Domain ID domain_id	1kf6B02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1060 — Fumarate Reductase Iron-sulfur Protein; Chain B, domain 2
Homologous superfamily homologous superfamily	10 — Alpha-helical ferredoxin

Domain ID domain_id	1kf6C00
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	1300 — 3 helical TM bundles of succinate and fumarate reductases
Homologous superfamily homologous superfamily	10 — Fumarate reductase/succinate dehydrogenase, transmembrane subunit

Domain ID domain_id	1kf6D00
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	1300 — 3 helical TM bundles of succinate and fumarate reductases
Homologous superfamily homologous superfamily	10 — Fumarate reductase/succinate dehydrogenase, transmembrane subunit

Domain ID domain_id	1kf6M01
Class class	3 — Alpha Beta
Architecture architecture	50 — 3-Layer(bba) Sandwich
Topology topology	50 — FAD/NAD(P)-binding domain
Homologous superfamily homologous superfamily	60 — FAD/NAD(P)-binding domain

Domain ID domain_id	1kf6M02
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	700 — Flavocytochrome C3; Chain A, domain 1
Homologous superfamily homologous superfamily	10 — Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain

Domain ID domain_id	1kf6M03
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
Homologous superfamily homologous superfamily	100 — Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain

Domain ID domain_id	1kf6M04
Class class	4 — Few Secondary Structures
Architecture architecture	10 — Irregular
Topology topology	80 — Rhinovirus 14, subunit 4
Homologous superfamily homologous superfamily	40 — succinate dehydrogenase protein domain

Domain ID domain_id	1kf6N01
Class class	3 — Alpha Beta
Architecture architecture	10 — Roll
Topology topology	20 — Ubiquitin-like (UB roll)
Homologous superfamily homologous superfamily	30 — Beta-grasp domain

Domain ID domain_id	1kf6N02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1060 — Fumarate Reductase Iron-sulfur Protein; Chain B, domain 2
Homologous superfamily homologous superfamily	10 — Alpha-helical ferredoxin

Domain ID domain_id	1kf6O00
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	1300 — 3 helical TM bundles of succinate and fumarate reductases
Homologous superfamily homologous superfamily	10 — Fumarate reductase/succinate dehydrogenase, transmembrane subunit

Domain ID domain_id	1kf6P00
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	1300 — 3 helical TM bundles of succinate and fumarate reductases
Homologous superfamily homologous superfamily	10 — Fumarate reductase/succinate dehydrogenase, transmembrane subunit

▶

Unit-cell length a length_a	96.505 Å
Unit-cell length b length_b	137.836 Å
Unit-cell length c length_c	273.451 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	8

Space group space_group	P 21 21 21
Space-group number int_tables_number	19
Crystal system crystal_system	—

Resolution resolution	2.700 Å
Low-resolution limit resolution_low	20.000 Å
R factor r_factor	—
R_work r_work	0.2310
R_free r_free	0.2800

E. coli Quinol-Fumarate Reductase with Bound Inhibitor HQNO

1. Protein Identity and Related Structures Protein Identity & Related Structures

FUMARATE REDUCTASE FLAVOPROTEIN

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

FUMARATE REDUCTASE IRON-SULFUR PROTEIN

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

FUMARATE REDUCTASE 15 KDA HYDROPHOBIC PROTEIN

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

FUMARATE REDUCTASE 13 KDA HYDROPHOBIC PROTEIN

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1kf6__assembly_1__model_1

1kf6__assembly_1__model_1 | I(q)

1kf6__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (15)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

Entity 3 Entity 3 polymer

Polymer Polymer

Source Source

Entity 4 Entity 4 polymer

Polymer Polymer

Source Source

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 non-polymer

Entity 7 Entity 7 non-polymer

Entity 8 Entity 8 non-polymer

Entity 9 Entity 9 non-polymer

Entity 10 Entity 10 non-polymer

Entity 11 Entity 11 non-polymer

Entity 12 Entity 12 non-polymer

Entity 13 Entity 13 non-polymer

Entity 14 Entity 14 non-polymer

Entity 15 Entity 15 water

6. Fold Classification (SCOP + CATH) 30 domains

SCOP 2.08 (14 domains)

CATH v4.4 (16 domains)

7. Citations (2)