1kij

Crystal structure of the 43K ATPase domain of Thermus thermophilus gyrase B in complex with novobiocin

Method: X-RAY DIFFRACTION Dmax: 90.2 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

SAXS profile for 1kij

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1kij
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1kij
沉积日期 deposition_date2001-12-03
结构标题 titleCrystal structure of the 43K ATPase domain of Thermus thermophilus gyrase B in complex with novobiocin
关键词 keywordstopoisomerase, gyrase B-coumarin complex, ISOMERASE; ISOMERASE
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier29.83
回转半径 Rg (电子) rg_electron28.79
零角强度 I(0) i0114714000.00
分子量 molecular_weight85291.0 kDa
排除体积 excluded_volume107260 ų
包络体积 envelope_volume135910 ų
水化壳体积 shell_volume38618 ų
包络直径 envelope_diameter96.5
壳层 Rg shell_rg36.72
包络 Rg envelope_rg28.67
形状 Rg shape_rg28.78
总 Rg total_rg29.61
总原子数 total_atoms6030
残基数 n_residues768
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax90.2
Rg (实空间) rg_real29.66
Rg 误差 (实空间) rg_real_error0.48
I(0) (实空间) i0_real1.1470e+08
I(0) 误差 (实空间) i0_real_error1.5580e+06
Rg (倒空间) rg_reciprocal29.73
I(0) (倒空间) i0_reciprocal114700000.0000
解质量估计 total_estimate0.9112
解质量评级 solution_quality EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary39.1
偏度 Skewness skewness0.093
峰度 Kurtosis kurtosis-0.613
角度范围 angular_range— – 0.2650 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha25090000.0000
实空间数据点数 n_real_points54
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.964; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.992; Smooth: 0.955

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (4)

6. 折叠分类 (SCOP + CATH) 8 domains

SCOP 2.08 (4 domains)

结构域编号 domain_idd1kija1
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.14 — Ribosomal protein S5 domain 2-like
超家族 Superfamily superfamilyd.14.1 — Ribosomal protein S5 domain 2-like
家族 Family familyd.14.1.3 — DNA gyrase/MutL, second domain
结构域编号 domain_idd1kija2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.122 — ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
超家族 Superfamily superfamilyd.122.1 — ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
家族 Family familyd.122.1.2 — DNA gyrase/MutL, N-terminal domain
结构域编号 domain_idd1kijb1
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.14 — Ribosomal protein S5 domain 2-like
超家族 Superfamily superfamilyd.14.1 — Ribosomal protein S5 domain 2-like
家族 Family familyd.14.1.3 — DNA gyrase/MutL, second domain
结构域编号 domain_idd1kijb2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.122 — ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
超家族 Superfamily superfamilyd.122.1 — ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
家族 Family familyd.122.1.2 — DNA gyrase/MutL, N-terminal domain

CATH v4.4 (4 domains)

结构域编号 domain_id1kijA01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology565 — Heat Shock Protein 90
同源超家族 H-superfamily homologous superfamily10 — Histidine kinase-like ATPase, C-terminal domain
结构域编号 domain_id1kijA02
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology230 — Ribosomal Protein S5; domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id1kijB01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology565 — Heat Shock Protein 90
同源超家族 H-superfamily homologous superfamily10 — Histidine kinase-like ATPase, C-terminal domain
结构域编号 domain_id1kijB02
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology230 — Ribosomal Protein S5; domain 2
同源超家族 H-superfamily homologous superfamily10

7. 引用文献 (1)

8. 文件与曲线 (10)