1m1g

Crystal Structure of Aquifex aeolicus N-utilization substance G (NusG), Space Group P2(1)

Method: X-RAY DIFFRACTION Dmax: 178.3 Å Quality: REASONABLE

SAXS 散射曲线 SAXS Profile

SAXS profile for 1m1g

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1m1g
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1m1g
沉积日期 deposition_date2002-06-19
结构标题 titleCrystal Structure of Aquifex aeolicus N-utilization substance G (NusG), Space Group P2(1)
关键词 keywords;Transcription termination, Antitermination, KOW domain, RNP motif, Immunoglobulin fold, Nucleic acid interaction, protein-protein interaction, TRANSCRIPTION ;; TRANSCRIPTION
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier49.00
回转半径 Rg (电子) rg_electron49.60
零角强度 I(0) i0165216000.00
分子量 molecular_weight108790.0 kDa
排除体积 excluded_volume138530 ų
包络体积 envelope_volume243960 ų
水化壳体积 shell_volume46192 ų
包络直径 envelope_diameter186.3
壳层 Rg shell_rg46.34
包络 Rg envelope_rg47.66
形状 Rg shape_rg49.59
总 Rg total_rg49.44
总原子数 total_atoms7640
残基数 n_residues965
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax178.3
Rg (实空间) rg_real49.54
Rg 误差 (实空间) rg_real_error2.26
I(0) (实空间) i0_real1.6520e+08
I(0) 误差 (实空间) i0_real_error3.1750e+06
Rg (倒空间) rg_reciprocal49.00
I(0) (倒空间) i0_reciprocal165100000.0000
解质量估计 total_estimate0.5944
解质量评级 solution_quality REASONABLE a REASONABLE solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary55.7
偏度 Skewness skewness0.535
峰度 Kurtosis kurtosis-0.010
角度范围 angular_range— – 0.1600 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha8442000.0000
实空间数据点数 n_real_points33
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.572; Stabil: 1.000; Sysdev: 0.087; Positv: 1.000; Valcen: 0.940; Smooth: 0.807

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (2)

6. 折叠分类 (SCOP + CATH) 24 domains

SCOP 2.08 (12 domains)

结构域编号 domain_idd1m1ga1
类 Class classb — All beta proteins
折叠类型 Fold foldb.114 — N-utilization substance G protein NusG, insert domain
超家族 Superfamily superfamilyb.114.1 — N-utilization substance G protein NusG, insert domain
家族 Family familyb.114.1.1 — N-utilization substance G protein NusG, insert domain
结构域编号 domain_idd1m1ga2
类 Class classb — All beta proteins
折叠类型 Fold foldb.34 — SH3-like barrel
超家族 Superfamily superfamilyb.34.5 — Translation proteins SH3-like domain
家族 Family familyb.34.5.4 — N-utilization substance G protein NusG, C-terminal domain
结构域编号 domain_idd1m1ga3
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.42 — N-utilization substance G protein NusG, N-terminal domain
家族 Family familyd.58.42.1 — N-utilization substance G protein NusG, N-terminal domain
结构域编号 domain_idd1m1gb1
类 Class classb — All beta proteins
折叠类型 Fold foldb.114 — N-utilization substance G protein NusG, insert domain
超家族 Superfamily superfamilyb.114.1 — N-utilization substance G protein NusG, insert domain
家族 Family familyb.114.1.1 — N-utilization substance G protein NusG, insert domain
结构域编号 domain_idd1m1gb2
类 Class classb — All beta proteins
折叠类型 Fold foldb.34 — SH3-like barrel
超家族 Superfamily superfamilyb.34.5 — Translation proteins SH3-like domain
家族 Family familyb.34.5.4 — N-utilization substance G protein NusG, C-terminal domain
结构域编号 domain_idd1m1gb3
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.42 — N-utilization substance G protein NusG, N-terminal domain
家族 Family familyd.58.42.1 — N-utilization substance G protein NusG, N-terminal domain
结构域编号 domain_idd1m1gc1
类 Class classb — All beta proteins
折叠类型 Fold foldb.114 — N-utilization substance G protein NusG, insert domain
超家族 Superfamily superfamilyb.114.1 — N-utilization substance G protein NusG, insert domain
家族 Family familyb.114.1.1 — N-utilization substance G protein NusG, insert domain
结构域编号 domain_idd1m1gc2
类 Class classb — All beta proteins
折叠类型 Fold foldb.34 — SH3-like barrel
超家族 Superfamily superfamilyb.34.5 — Translation proteins SH3-like domain
家族 Family familyb.34.5.4 — N-utilization substance G protein NusG, C-terminal domain
结构域编号 domain_idd1m1gc3
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.42 — N-utilization substance G protein NusG, N-terminal domain
家族 Family familyd.58.42.1 — N-utilization substance G protein NusG, N-terminal domain
结构域编号 domain_idd1m1gd1
类 Class classb — All beta proteins
折叠类型 Fold foldb.114 — N-utilization substance G protein NusG, insert domain
超家族 Superfamily superfamilyb.114.1 — N-utilization substance G protein NusG, insert domain
家族 Family familyb.114.1.1 — N-utilization substance G protein NusG, insert domain
结构域编号 domain_idd1m1gd2
类 Class classb — All beta proteins
折叠类型 Fold foldb.34 — SH3-like barrel
超家族 Superfamily superfamilyb.34.5 — Translation proteins SH3-like domain
家族 Family familyb.34.5.4 — N-utilization substance G protein NusG, C-terminal domain
结构域编号 domain_idd1m1gd3
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.42 — N-utilization substance G protein NusG, N-terminal domain
家族 Family familyd.58.42.1 — N-utilization substance G protein NusG, N-terminal domain

CATH v4.4 (12 domains)

结构域编号 domain_id1m1gA01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily940 — NusG, N-terminal domain
结构域编号 domain_id1m1gA02
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology320 — mini-chromosome maintenance (MCM) complex, domain 2
同源超家族 H-superfamily homologous superfamily10 — N-utilization substance G protein NusG, insert domain
结构域编号 domain_id1m1gA03
类 Class class2 — Mainly Beta
架构 Architecture architecture30 — Roll
拓扑 Topology topology30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily30
结构域编号 domain_id1m1gB01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily940 — NusG, N-terminal domain
结构域编号 domain_id1m1gB02
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology320 — mini-chromosome maintenance (MCM) complex, domain 2
同源超家族 H-superfamily homologous superfamily10 — N-utilization substance G protein NusG, insert domain
结构域编号 domain_id1m1gB03
类 Class class2 — Mainly Beta
架构 Architecture architecture30 — Roll
拓扑 Topology topology30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily30
结构域编号 domain_id1m1gC01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily940 — NusG, N-terminal domain
结构域编号 domain_id1m1gC02
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology320 — mini-chromosome maintenance (MCM) complex, domain 2
同源超家族 H-superfamily homologous superfamily10 — N-utilization substance G protein NusG, insert domain
结构域编号 domain_id1m1gC03
类 Class class2 — Mainly Beta
架构 Architecture architecture30 — Roll
拓扑 Topology topology30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily30
结构域编号 domain_id1m1gD01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily940 — NusG, N-terminal domain
结构域编号 domain_id1m1gD02
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology320 — mini-chromosome maintenance (MCM) complex, domain 2
同源超家族 H-superfamily homologous superfamily10 — N-utilization substance G protein NusG, insert domain
结构域编号 domain_id1m1gD03
类 Class class2 — Mainly Beta
架构 Architecture architecture30 — Roll
拓扑 Topology topology30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily30

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