SAXSdb — 1m1l

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Suppressor of Fused

Homo sapiens

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein homooligomer Homooligomer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count	Chain A； UniProt 27–262 Chain B； UniProt 27–262 Chain C； UniProt 27–262 Chain D； UniProt 27–262	Fragment：N-terminal domain (Residues 27-262)	No other associated polymer	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6；279 K；1 M LiCl, 0.1 M NaCitrate pH 5.0- 6.0, 10% w/v PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 279K	Resolution 2.65 Å R-free 0.256

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 1M1L	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
4BL8 Crystal structure of full-length human Suppressor of fused (SUFU) Deposited 2013-05-02	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric	Chain A 32–483（452 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483	Mutation：YES	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；PROTEIN (12 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS CRYSTALLISED AT 4OC BY HANGING DROP VAPOUR DIFFUSION WITH 0.2 M K/NA TARTRATE, 0.1 M BIS-TRIS PROPANE PH 8.5 AND 16% (V/V) PEG 3350 (AT A PROTEIN:MOTHER LIQUOR RATIO OF 2:1)	Resolution 3.04 Å R-free 0.246
4BL8 Crystal structure of full-length human Suppressor of fused (SUFU) Deposited 2013-05-02	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric	Chain B 32–483（452 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483	Mutation：YES	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；PROTEIN (12 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS CRYSTALLISED AT 4OC BY HANGING DROP VAPOUR DIFFUSION WITH 0.2 M K/NA TARTRATE, 0.1 M BIS-TRIS PROPANE PH 8.5 AND 16% (V/V) PEG 3350 (AT A PROTEIN:MOTHER LIQUOR RATIO OF 2:1)	Resolution 3.04 Å R-free 0.246
4BL9 Crystal structure of full-length human Suppressor of fused (SUFU) mutant lacking a regulatory subdomain (crystal form I) Deposited 2013-05-02	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric	Chain A 32–278（247 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483 Chain A 361–483（123 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483	Mutation：YES Mutation：YES	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；277 K；PROTEIN (6.5 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS CRYSTALLISED AT 4C BY HANGING DROP VAPOUR DIFFUSION WITH 0.08 M NA-CACODYLATE (PH 6.6), 20% (V/V) GLYCEROL, 160 MM CA(OAC)2 AND 9% (V/V) PEG 8000	Resolution 2.80 Å R-free 0.234
4BL9 Crystal structure of full-length human Suppressor of fused (SUFU) mutant lacking a regulatory subdomain (crystal form I) Deposited 2013-05-02	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric	Chain B 32–278（247 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483 Chain B 361–483（123 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483	Mutation：YES Mutation：YES	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；277 K；PROTEIN (6.5 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS CRYSTALLISED AT 4C BY HANGING DROP VAPOUR DIFFUSION WITH 0.08 M NA-CACODYLATE (PH 6.6), 20% (V/V) GLYCEROL, 160 MM CA(OAC)2 AND 9% (V/V) PEG 8000	Resolution 2.80 Å R-free 0.234
4BL9 Crystal structure of full-length human Suppressor of fused (SUFU) mutant lacking a regulatory subdomain (crystal form I) Deposited 2013-05-02	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric	Chain C 32–278（247 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483 Chain C 361–483（123 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483	Mutation：YES Mutation：YES	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；277 K；PROTEIN (6.5 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS CRYSTALLISED AT 4C BY HANGING DROP VAPOUR DIFFUSION WITH 0.08 M NA-CACODYLATE (PH 6.6), 20% (V/V) GLYCEROL, 160 MM CA(OAC)2 AND 9% (V/V) PEG 8000	Resolution 2.80 Å R-free 0.234
4BL9 Crystal structure of full-length human Suppressor of fused (SUFU) mutant lacking a regulatory subdomain (crystal form I) Deposited 2013-05-02	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 4 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric	Chain D 32–278（247 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483 Chain D 361–483（123 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483	Mutation：YES Mutation：YES	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；277 K；PROTEIN (6.5 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS CRYSTALLISED AT 4C BY HANGING DROP VAPOUR DIFFUSION WITH 0.08 M NA-CACODYLATE (PH 6.6), 20% (V/V) GLYCEROL, 160 MM CA(OAC)2 AND 9% (V/V) PEG 8000	Resolution 2.80 Å R-free 0.234
4BLA Crystal structure of full-length human Suppressor of fused (SUFU) mutant lacking a regulatory subdomain (crystal form II) Deposited 2013-05-02	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric	Chain A 32–278（247 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483 Chain A 361–483（123 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483	Mutation：YES Mutation：YES	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (11.6 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT) WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 0.1 M NA-HEPES PH 7.5, 17% (V/V) PEG 3350, 0.2 M NACL.	Resolution 3.50 Å R-free 0.293
4BLA Crystal structure of full-length human Suppressor of fused (SUFU) mutant lacking a regulatory subdomain (crystal form II) Deposited 2013-05-02	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric	Chain B 32–278（247 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483 Chain B 361–483（123 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483	Mutation：YES Mutation：YES	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (11.6 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT) WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 0.1 M NA-HEPES PH 7.5, 17% (V/V) PEG 3350, 0.2 M NACL.	Resolution 3.50 Å R-free 0.293
4BLA Crystal structure of full-length human Suppressor of fused (SUFU) mutant lacking a regulatory subdomain (crystal form II) Deposited 2013-05-02	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric	Chain C 32–278（247 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483 Chain C 361–483（123 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483	Mutation：YES Mutation：YES	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (11.6 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT) WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 0.1 M NA-HEPES PH 7.5, 17% (V/V) PEG 3350, 0.2 M NACL.	Resolution 3.50 Å R-free 0.293
4BLA Crystal structure of full-length human Suppressor of fused (SUFU) mutant lacking a regulatory subdomain (crystal form II) Deposited 2013-05-02	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 4 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric	Chain D 32–278（247 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483 Chain D 361–483（123 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483	Mutation：YES Mutation：YES	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (11.6 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT) WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 0.1 M NA-HEPES PH 7.5, 17% (V/V) PEG 3350, 0.2 M NACL.	Resolution 3.50 Å R-free 0.293
4BLB Crystal structure of a human Suppressor of fused (SUFU)-GLI1p complex Deposited 2013-05-02	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric	Chain C 32–278（247 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483 Chain C 361–453（93 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483 Chain C 457–483（27 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483	Mutation：YES Mutation：YES Mutation：YES	ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (10 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS MIXED IN A 1:1 MOLAR RATIO WITH ZN(OAC)2 AND A 1:4 MOLAR RATIO WITH GLI1 PEPTIDE. THE COMPLEX WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 1:1 OR 2:1 DROPS OF PROTEIN:WELL SOLUTION (14-18% (V/V) PEG 3350, AND 0.2 M NA FORMATE)	Resolution 2.80 Å R-free 0.234
4BLB Crystal structure of a human Suppressor of fused (SUFU)-GLI1p complex Deposited 2013-05-02	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 32–278（247 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483 Chain A 361–453（93 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483 Chain A 457–483（27 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483	Mutation：YES Mutation：YES Mutation：YES	ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (10 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS MIXED IN A 1:1 MOLAR RATIO WITH ZN(OAC)2 AND A 1:4 MOLAR RATIO WITH GLI1 PEPTIDE. THE COMPLEX WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 1:1 OR 2:1 DROPS OF PROTEIN:WELL SOLUTION (14-18% (V/V) PEG 3350, AND 0.2 M NA FORMATE)	Resolution 2.80 Å R-free 0.234
4BLB Crystal structure of a human Suppressor of fused (SUFU)-GLI1p complex Deposited 2013-05-02	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric	Chain D 32–278（247 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483 Chain D 361–453（93 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483 Chain D 457–483（27 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483	Mutation：YES Mutation：YES Mutation：YES	ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (10 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS MIXED IN A 1:1 MOLAR RATIO WITH ZN(OAC)2 AND A 1:4 MOLAR RATIO WITH GLI1 PEPTIDE. THE COMPLEX WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 1:1 OR 2:1 DROPS OF PROTEIN:WELL SOLUTION (14-18% (V/V) PEG 3350, AND 0.2 M NA FORMATE)	Resolution 2.80 Å R-free 0.234
4BLB Crystal structure of a human Suppressor of fused (SUFU)-GLI1p complex Deposited 2013-05-02	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 4 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 32–278（247 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483 Chain B 361–453（93 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483 Chain B 457–483（27 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483	Mutation：YES Mutation：YES Mutation：YES	ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (10 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS MIXED IN A 1:1 MOLAR RATIO WITH ZN(OAC)2 AND A 1:4 MOLAR RATIO WITH GLI1 PEPTIDE. THE COMPLEX WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 1:1 OR 2:1 DROPS OF PROTEIN:WELL SOLUTION (14-18% (V/V) PEG 3350, AND 0.2 M NA FORMATE)	Resolution 2.80 Å R-free 0.234
4BLD Crystal structure of a human Suppressor of fused (SUFU)-GLI3p complex Deposited 2013-05-02	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 32–278（247 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483 Chain A 361–453（93 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483 Chain A 457–483（27 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483	Mutation：YES Mutation：YES Mutation：YES	ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (10 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS MIXED IN A 1:1 MOLAR RATIO WITH ZN(OAC)2 AND A 1:4 MOLAR RATIO WITH GLI3 PEPTIDE. THE COMPLEX WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 1:1 OR 2:1 DROPS OF PROTEIN:WELL SOLUTION (14-18% (V/V) PEG 3350 AND 0.2 M NA FORMATE)	Resolution 2.80 Å R-free 0.234
4BLD Crystal structure of a human Suppressor of fused (SUFU)-GLI3p complex Deposited 2013-05-02	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric	Chain C 32–278（247 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483 Chain C 361–453（93 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483 Chain C 457–483（27 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483	Mutation：YES Mutation：YES Mutation：YES	ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (10 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS MIXED IN A 1:1 MOLAR RATIO WITH ZN(OAC)2 AND A 1:4 MOLAR RATIO WITH GLI3 PEPTIDE. THE COMPLEX WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 1:1 OR 2:1 DROPS OF PROTEIN:WELL SOLUTION (14-18% (V/V) PEG 3350 AND 0.2 M NA FORMATE)	Resolution 2.80 Å R-free 0.234
4BLD Crystal structure of a human Suppressor of fused (SUFU)-GLI3p complex Deposited 2013-05-02	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 32–278（247 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483 Chain B 361–453（93 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483 Chain B 457–483（27 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483	Mutation：YES Mutation：YES Mutation：YES	ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (10 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS MIXED IN A 1:1 MOLAR RATIO WITH ZN(OAC)2 AND A 1:4 MOLAR RATIO WITH GLI3 PEPTIDE. THE COMPLEX WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 1:1 OR 2:1 DROPS OF PROTEIN:WELL SOLUTION (14-18% (V/V) PEG 3350 AND 0.2 M NA FORMATE)	Resolution 2.80 Å R-free 0.234
4BLD Crystal structure of a human Suppressor of fused (SUFU)-GLI3p complex Deposited 2013-05-02	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 4 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric	Chain D 32–278（247 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483 Chain D 361–453（93 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483 Chain D 457–483（27 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483	Mutation：YES Mutation：YES Mutation：YES	ZN ZINC ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (10 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS MIXED IN A 1:1 MOLAR RATIO WITH ZN(OAC)2 AND A 1:4 MOLAR RATIO WITH GLI3 PEPTIDE. THE COMPLEX WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 1:1 OR 2:1 DROPS OF PROTEIN:WELL SOLUTION (14-18% (V/V) PEG 3350 AND 0.2 M NA FORMATE)	Resolution 2.80 Å R-free 0.234
4KM8 Crystal structure of Sufud60 Deposited 2013-05-08	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–285（285 aa） Fragment：UNP RESIDUES 1-285, 346-484 Chain A 346–484（139 aa） Fragment：UNP RESIDUES 1-285, 346-484	Mutation：Deletion 286-345 Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：Deletion 286-345 Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；287 K；8% PEG 3350, 0.15M Ammonium Tartrate, vapor diffusion, hanging drop, temperature 287K	Resolution 2.26 Å R-free 0.259
4KM9 Crystal structure of human Suppressor of Fused Deposited 2013-05-08	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–484（484 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；281 K；20% PEG 3350, 0.2M Potassium chloride, vapor diffusion, hanging drop, temperature 281K	Resolution 3.19 Å R-free 0.305
4KMD Crystal structure of Sufud60-Gli1p Deposited 2013-05-08	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 1–285（285 aa） Fragment：UNP RESIDUES 1-285, 346-484 Chain A 346–484（139 aa） Fragment：UNP RESIDUES 1-285, 346-484	Not recorded	GOL GLYCEROL × 6 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；20% PEG 3350, 0.2M Magnesium Chloride, vapor diffusion, hanging drop, temperature 277K	Resolution 1.70 Å R-free 0.222
4KMD Crystal structure of Sufud60-Gli1p Deposited 2013-05-08	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain A 1–285（285 aa） Fragment：UNP RESIDUES 1-285, 346-484 Chain A 346–484（139 aa） Fragment：UNP RESIDUES 1-285, 346-484	Not recorded	GOL GLYCEROL × 12 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；20% PEG 3350, 0.2M Magnesium Chloride, vapor diffusion, hanging drop, temperature 277K	Resolution 1.70 Å R-free 0.222
4KMH Crystal structure of Suppressor of Fused d20 Deposited 2013-05-08	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1–305（305 aa） Fragment：UNP RESIDUES 1-305, 326-484 Chain A 326–484（159 aa） Fragment：UNP RESIDUES 1-305, 326-484 Chain B 1–305（305 aa） Fragment：UNP RESIDUES 1-305, 326-484 Chain B 326–484（159 aa） Fragment：UNP RESIDUES 1-305, 326-484	Mutation：DELETION RESIDUES 306-325 Mutation：DELETION RESIDUES 306-325 Mutation：DELETION RESIDUES 306-325 Mutation：DELETION RESIDUES 306-325	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；287 K；16% PEG 3350, 0.1M Ammonium Tartrate, vapor diffusion, hanging drop, temperature 287K	Resolution 3.04 Å R-free 0.277

9 other PDB entries and 23 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	SUFU_HUMAN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–236； UniProt 27–262 Author chain B； PDBConstruct 1–236； UniProt 27–262 Author chain C； PDBConstruct 1–236； UniProt 27–262 Author chain D； PDBConstruct 1–236； UniProt 27–262

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	1m1l
Deposition date deposition_date	2002-06-19
Structure title title	Human Suppressor of Fused (N-terminal domain)
Keywords keywords	Gene regulation, Hedgehog signaling, signal transduction, fused, SIGNALING PROTEIN; SIGNALING PROTEIN
Experimental Method method	X-RAY DIFFRACTION

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	32.70 Å
Radius of gyration Rg (electron density) rg_electron	31.60 Å
Forward intensity I(0) i0	179311000.00
Molecular weight molecular_weight	105900.0 kDa
Excluded volume excluded_volume	131810 Å³
Envelope volume envelope_volume	165360 Å³
Hydration-shell volume shell_volume	42886 Å³
Envelope diameter envelope_diameter	102.0 Å
Shell Rg shell_rg	39.24 Å
Envelope Rg envelope_rg	31.27 Å
Shape Rg shape_rg	31.61 Å
Total Rg total_rg	32.20 Å
Total atoms total_atoms	7486
Residues n_residues	940
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	101.4 Å
Rg (real space) rg_real	32.51 Å
Rg uncertainty (real space) rg_real_error	0.63 Å
I(0) (real space) i0_real	1.7930e+08
I(0) uncertainty (real space) i0_real_error	2.6960e+06
Rg (reciprocal space) rg_reciprocal	32.60 Å
I(0) (reciprocal space) i0_reciprocal	179300000.0000
Solution quality estimate total_estimate	0.9085
Solution quality rating solution_quality	EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks	2
Primary peak position r_peak_primary	42.2 Å
Skewness Skewness skewness	0.106
Kurtosis Kurtosis kurtosis	-0.612
Angular range angular_range	— – 0.2400 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	34320000.0000
Real-space data points n_real_points	49
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.953; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.997; Smooth: 0.949

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (2)

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7. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (4 domains)

Domain ID domain_id	d1m1la_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.260 — Suppressor of Fused, N-terminal domain
Superfamily Superfamily superfamily	d.260.1 — Suppressor of Fused, N-terminal domain
Family Family family	d.260.1.1 — Suppressor of Fused, N-terminal domain

Domain ID domain_id	d1m1lb_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.260 — Suppressor of Fused, N-terminal domain
Superfamily Superfamily superfamily	d.260.1 — Suppressor of Fused, N-terminal domain
Family Family family	d.260.1.1 — Suppressor of Fused, N-terminal domain

Domain ID domain_id	d1m1lc_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.260 — Suppressor of Fused, N-terminal domain
Superfamily Superfamily superfamily	d.260.1 — Suppressor of Fused, N-terminal domain
Family Family family	d.260.1.1 — Suppressor of Fused, N-terminal domain

Domain ID domain_id	d1m1ld_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.260 — Suppressor of Fused, N-terminal domain
Superfamily Superfamily superfamily	d.260.1 — Suppressor of Fused, N-terminal domain
Family Family family	d.260.1.1 — Suppressor of Fused, N-terminal domain

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8. Citations (1)

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Unit-cell length a length_a	173.025 Å
Unit-cell length b length_b	173.025 Å
Unit-cell length c length_c	290.833 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	120.00 °
Molecules per unit cell Z z_pdb	72

Space group space_group	H 3 2
Space-group number int_tables_number	155
Crystal system crystal_system	—

Resolution resolution	2.650 Å
Low-resolution limit resolution_low	29.810 Å
R factor r_factor	—
R_work r_work	0.2235
R_free r_free	0.2564

Description description	Suppressor of Fused
Molecular weight formula_weight	26592.7 kDa
Source method src_method	man
Copy count entity_count	4

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D
Atom count atom_count	7832

Human Suppressor of Fused (N-terminal domain)

1. Protein Identity and Related Structures Protein Identity & Related Structures

Suppressor of Fused

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

6. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

来源 Source

Entity 2 Entity 2 water

7. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (4 domains)

8. Citations (1)

9. Files and Curves (10)

Files (7)

Curves (3)

来源生物 organism	Homo sapiens
通用名 common_name	human
NCBI Class编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Escherichia coli

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	346

fit	/app/data/saxs_computation/m1/1m1l/1m1l.fit
pddf	/app/data/pr_computation/m1/1m1l/1m1l.pr.out
profile	/app/data/saxs_computation/m1/1m1l/1m1l.dat