← Back to search
4kmh

Crystal structure of Suppressor of Fused d20

Method: X-RAY DIFFRACTION Dmax: 112.4 Å Quality: GOOD
▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

Suppressor of fused homolog

Homo sapiens

UniProt Q9UMX1

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein homooligomer Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain A； UniProt 1–305 Chain A； UniProt 326–484 Chain B； UniProt 1–305 Chain B； UniProt 326–484 Fragment：UNP RESIDUES 1-305, 326-484 Mutation：DELETION RESIDUES 306-325 No other associated polymer X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；287 K；16% PEG 3350, 0.1M Ammonium Tartrate, vapor diffusion, hanging drop, temperature 287K Resolution 3.04 Å R-free 0.277

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4KMH Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1M1L Human Suppressor of Fused (N-terminal domain) Deposited 2002-06-19 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 27–262（236 aa） Fragment：N-terminal domain (Residues 27-262)
Chain B 27–262（236 aa） Fragment：N-terminal domain (Residues 27-262)
Chain C 27–262（236 aa） Fragment：N-terminal domain (Residues 27-262)
Chain D 27–262（236 aa） Fragment：N-terminal domain (Residues 27-262)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；279 K；1 M LiCl, 0.1 M NaCitrate pH 5.0- 6.0, 10% w/v PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 279K
Resolution 2.65 Å R-free 0.256
4BL8 Crystal structure of full-length human Suppressor of fused (SUFU) Deposited 2013-05-02 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 32–483（452 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483
Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；PROTEIN (12 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS CRYSTALLISED AT 4OC BY HANGING DROP VAPOUR DIFFUSION WITH 0.2 M K/NA TARTRATE, 0.1 M BIS-TRIS PROPANE PH 8.5 AND 16% (V/V) PEG 3350 (AT A PROTEIN:MOTHER LIQUOR RATIO OF 2:1)
Resolution 3.04 Å R-free 0.246
4BL8 Crystal structure of full-length human Suppressor of fused (SUFU) Deposited 2013-05-02 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain B 32–483（452 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483
Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；PROTEIN (12 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS CRYSTALLISED AT 4OC BY HANGING DROP VAPOUR DIFFUSION WITH 0.2 M K/NA TARTRATE, 0.1 M BIS-TRIS PROPANE PH 8.5 AND 16% (V/V) PEG 3350 (AT A PROTEIN:MOTHER LIQUOR RATIO OF 2:1)
Resolution 3.04 Å R-free 0.246
4BL9 Crystal structure of full-length human Suppressor of fused (SUFU) mutant lacking a regulatory subdomain (crystal form I) Deposited 2013-05-02 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 32–278（247 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483
Chain A 361–483（123 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483
Mutation：YES Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；277 K；PROTEIN (6.5 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS CRYSTALLISED AT 4C BY HANGING DROP VAPOUR DIFFUSION WITH 0.08 M NA-CACODYLATE (PH 6.6), 20% (V/V) GLYCEROL, 160 MM CA(OAC)2 AND 9% (V/V) PEG 8000
Resolution 2.80 Å R-free 0.234
4BL9 Crystal structure of full-length human Suppressor of fused (SUFU) mutant lacking a regulatory subdomain (crystal form I) Deposited 2013-05-02 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain B 32–278（247 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483
Chain B 361–483（123 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483
Mutation：YES Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；277 K；PROTEIN (6.5 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS CRYSTALLISED AT 4C BY HANGING DROP VAPOUR DIFFUSION WITH 0.08 M NA-CACODYLATE (PH 6.6), 20% (V/V) GLYCEROL, 160 MM CA(OAC)2 AND 9% (V/V) PEG 8000
Resolution 2.80 Å R-free 0.234
4BL9 Crystal structure of full-length human Suppressor of fused (SUFU) mutant lacking a regulatory subdomain (crystal form I) Deposited 2013-05-02 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 3 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain C 32–278（247 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483
Chain C 361–483（123 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483
Mutation：YES Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；277 K；PROTEIN (6.5 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS CRYSTALLISED AT 4C BY HANGING DROP VAPOUR DIFFUSION WITH 0.08 M NA-CACODYLATE (PH 6.6), 20% (V/V) GLYCEROL, 160 MM CA(OAC)2 AND 9% (V/V) PEG 8000
Resolution 2.80 Å R-free 0.234
4BL9 Crystal structure of full-length human Suppressor of fused (SUFU) mutant lacking a regulatory subdomain (crystal form I) Deposited 2013-05-02 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 4 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain D 32–278（247 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483
Chain D 361–483（123 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483
Mutation：YES Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；277 K；PROTEIN (6.5 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS CRYSTALLISED AT 4C BY HANGING DROP VAPOUR DIFFUSION WITH 0.08 M NA-CACODYLATE (PH 6.6), 20% (V/V) GLYCEROL, 160 MM CA(OAC)2 AND 9% (V/V) PEG 8000
Resolution 2.80 Å R-free 0.234
4BLA Crystal structure of full-length human Suppressor of fused (SUFU) mutant lacking a regulatory subdomain (crystal form II) Deposited 2013-05-02 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 32–278（247 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483
Chain A 361–483（123 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483
Mutation：YES Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (11.6 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT) WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 0.1 M NA-HEPES PH 7.5, 17% (V/V) PEG 3350, 0.2 M NACL.
Resolution 3.50 Å R-free 0.293
4BLA Crystal structure of full-length human Suppressor of fused (SUFU) mutant lacking a regulatory subdomain (crystal form II) Deposited 2013-05-02 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain B 32–278（247 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483
Chain B 361–483（123 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483
Mutation：YES Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (11.6 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT) WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 0.1 M NA-HEPES PH 7.5, 17% (V/V) PEG 3350, 0.2 M NACL.
Resolution 3.50 Å R-free 0.293
4BLA Crystal structure of full-length human Suppressor of fused (SUFU) mutant lacking a regulatory subdomain (crystal form II) Deposited 2013-05-02 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 3 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain C 32–278（247 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483
Chain C 361–483（123 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483
Mutation：YES Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (11.6 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT) WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 0.1 M NA-HEPES PH 7.5, 17% (V/V) PEG 3350, 0.2 M NACL.
Resolution 3.50 Å R-free 0.293
4BLA Crystal structure of full-length human Suppressor of fused (SUFU) mutant lacking a regulatory subdomain (crystal form II) Deposited 2013-05-02 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 4 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain D 32–278（247 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483
Chain D 361–483（123 aa） Fragment：MBPP RESIDUES 29-387,SUFUH RESIDUES 32-278,361-483
Mutation：YES Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (11.6 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT) WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 0.1 M NA-HEPES PH 7.5, 17% (V/V) PEG 3350, 0.2 M NACL.
Resolution 3.50 Å R-free 0.293
4BLB Crystal structure of a human Suppressor of fused (SUFU)-GLI1p complex Deposited 2013-05-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 32–278（247 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Chain C 361–453（93 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Chain C 457–483（27 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Mutation：YES Mutation：YES Mutation：YES ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (10 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS MIXED IN A 1:1 MOLAR RATIO WITH ZN(OAC)2 AND A 1:4 MOLAR RATIO WITH GLI1 PEPTIDE. THE COMPLEX WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 1:1 OR 2:1 DROPS OF PROTEIN:WELL SOLUTION (14-18% (V/V) PEG 3350, AND 0.2 M NA FORMATE)
Resolution 2.80 Å R-free 0.234
4BLB Crystal structure of a human Suppressor of fused (SUFU)-GLI1p complex Deposited 2013-05-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 32–278（247 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Chain A 361–453（93 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Chain A 457–483（27 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Mutation：YES Mutation：YES Mutation：YES ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (10 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS MIXED IN A 1:1 MOLAR RATIO WITH ZN(OAC)2 AND A 1:4 MOLAR RATIO WITH GLI1 PEPTIDE. THE COMPLEX WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 1:1 OR 2:1 DROPS OF PROTEIN:WELL SOLUTION (14-18% (V/V) PEG 3350, AND 0.2 M NA FORMATE)
Resolution 2.80 Å R-free 0.234
4BLB Crystal structure of a human Suppressor of fused (SUFU)-GLI1p complex Deposited 2013-05-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 32–278（247 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Chain D 361–453（93 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Chain D 457–483（27 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Mutation：YES Mutation：YES Mutation：YES ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (10 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS MIXED IN A 1:1 MOLAR RATIO WITH ZN(OAC)2 AND A 1:4 MOLAR RATIO WITH GLI1 PEPTIDE. THE COMPLEX WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 1:1 OR 2:1 DROPS OF PROTEIN:WELL SOLUTION (14-18% (V/V) PEG 3350, AND 0.2 M NA FORMATE)
Resolution 2.80 Å R-free 0.234
4BLB Crystal structure of a human Suppressor of fused (SUFU)-GLI1p complex Deposited 2013-05-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 32–278（247 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Chain B 361–453（93 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Chain B 457–483（27 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Mutation：YES Mutation：YES Mutation：YES ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (10 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS MIXED IN A 1:1 MOLAR RATIO WITH ZN(OAC)2 AND A 1:4 MOLAR RATIO WITH GLI1 PEPTIDE. THE COMPLEX WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 1:1 OR 2:1 DROPS OF PROTEIN:WELL SOLUTION (14-18% (V/V) PEG 3350, AND 0.2 M NA FORMATE)
Resolution 2.80 Å R-free 0.234
4BLD Crystal structure of a human Suppressor of fused (SUFU)-GLI3p complex Deposited 2013-05-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 32–278（247 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Chain A 361–453（93 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Chain A 457–483（27 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Mutation：YES Mutation：YES Mutation：YES ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (10 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS MIXED IN A 1:1 MOLAR RATIO WITH ZN(OAC)2 AND A 1:4 MOLAR RATIO WITH GLI3 PEPTIDE. THE COMPLEX WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 1:1 OR 2:1 DROPS OF PROTEIN:WELL SOLUTION (14-18% (V/V) PEG 3350 AND 0.2 M NA FORMATE)
Resolution 2.80 Å R-free 0.234
4BLD Crystal structure of a human Suppressor of fused (SUFU)-GLI3p complex Deposited 2013-05-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 32–278（247 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Chain C 361–453（93 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Chain C 457–483（27 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Mutation：YES Mutation：YES Mutation：YES ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (10 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS MIXED IN A 1:1 MOLAR RATIO WITH ZN(OAC)2 AND A 1:4 MOLAR RATIO WITH GLI3 PEPTIDE. THE COMPLEX WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 1:1 OR 2:1 DROPS OF PROTEIN:WELL SOLUTION (14-18% (V/V) PEG 3350 AND 0.2 M NA FORMATE)
Resolution 2.80 Å R-free 0.234
4BLD Crystal structure of a human Suppressor of fused (SUFU)-GLI3p complex Deposited 2013-05-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 32–278（247 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Chain B 361–453（93 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Chain B 457–483（27 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Mutation：YES Mutation：YES Mutation：YES ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (10 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS MIXED IN A 1:1 MOLAR RATIO WITH ZN(OAC)2 AND A 1:4 MOLAR RATIO WITH GLI3 PEPTIDE. THE COMPLEX WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 1:1 OR 2:1 DROPS OF PROTEIN:WELL SOLUTION (14-18% (V/V) PEG 3350 AND 0.2 M NA FORMATE)
Resolution 2.80 Å R-free 0.234
4BLD Crystal structure of a human Suppressor of fused (SUFU)-GLI3p complex Deposited 2013-05-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 32–278（247 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Chain D 361–453（93 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Chain D 457–483（27 aa） Fragment：MBPP RESIDUES 29-392,SUFUH RESIDUES 32-278,361-483
Mutation：YES Mutation：YES Mutation：YES ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PROTEIN (10 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS MIXED IN A 1:1 MOLAR RATIO WITH ZN(OAC)2 AND A 1:4 MOLAR RATIO WITH GLI3 PEPTIDE. THE COMPLEX WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 1:1 OR 2:1 DROPS OF PROTEIN:WELL SOLUTION (14-18% (V/V) PEG 3350 AND 0.2 M NA FORMATE)
Resolution 2.80 Å R-free 0.234
4KM8 Crystal structure of Sufud60 Deposited 2013-05-08 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–285（285 aa） Fragment：UNP RESIDUES 1-285, 346-484
Chain A 346–484（139 aa） Fragment：UNP RESIDUES 1-285, 346-484
Mutation：Deletion 286-345 Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：Deletion 286-345 Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；287 K；8% PEG 3350, 0.15M Ammonium Tartrate, vapor diffusion, hanging drop, temperature 287K
Resolution 2.26 Å R-free 0.259
4KM9 Crystal structure of human Suppressor of Fused Deposited 2013-05-08 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–484（484 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；281 K；20% PEG 3350, 0.2M Potassium chloride, vapor diffusion, hanging drop, temperature 281K
Resolution 3.19 Å R-free 0.305
4KMD Crystal structure of Sufud60-Gli1p Deposited 2013-05-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–285（285 aa） Fragment：UNP RESIDUES 1-285, 346-484
Chain A 346–484（139 aa） Fragment：UNP RESIDUES 1-285, 346-484
Not recorded GOL GLYCEROL × 6 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；20% PEG 3350, 0.2M Magnesium Chloride, vapor diffusion, hanging drop, temperature 277K
Resolution 1.70 Å R-free 0.222
4KMD Crystal structure of Sufud60-Gli1p Deposited 2013-05-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–285（285 aa） Fragment：UNP RESIDUES 1-285, 346-484
Chain A 346–484（139 aa） Fragment：UNP RESIDUES 1-285, 346-484
Not recorded GOL GLYCEROL × 12 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；20% PEG 3350, 0.2M Magnesium Chloride, vapor diffusion, hanging drop, temperature 277K
Resolution 1.70 Å R-free 0.222

9 other PDB entries and 23 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name SUFU_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 21–325； UniProt 1–305 Author chain A； PDBConstruct 326–484； UniProt 326–484 Author chain B； PDBConstruct 21–325； UniProt 1–305 Author chain B； PDBConstruct 326–484； UniProt 326–484

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 4kmh

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 4kmh
Download Download
▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id4kmh
Deposition date deposition_date2013-05-08
Structure title titleCrystal structure of Suppressor of Fused d20
Keywords keywordshelix and beta strand, PROTEIN BINDING; PROTEIN BINDING
Experimental Method methodX-RAY DIFFRACTION
▼

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier32.34 Å
Radius of gyration Rg (electron density) rg_electron31.58 Å
Forward intensity I(0) i0117519000.00
Molecular weight molecular_weight85811.0 kDa
Excluded volume excluded_volume107170 ų
Envelope volume envelope_volume146900 ų
Hydration-shell volume shell_volume38112 ų
Envelope diameter envelope_diameter121.6 Å
Shell Rg shell_rg38.83 Å
Envelope Rg envelope_rg32.14 Å
Shape Rg shape_rg31.56 Å
Total Rg total_rg32.30 Å
Total atoms total_atoms6063
Residues n_residues772
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
▼

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax112.4 Å
Rg (real space) rg_real32.26 Å
Rg uncertainty (real space) rg_real_error1.28 Å
I(0) (real space) i0_real1.1750e+08
I(0) uncertainty (real space) i0_real_error2.0140e+06
Rg (reciprocal space) rg_reciprocal32.30 Å
I(0) (reciprocal space) i0_reciprocal117500000.0000
Solution quality estimate total_estimate0.8478
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks3
Primary peak position r_peak_primary43.6 Å
Skewness Skewness skewness0.234
Kurtosis Kurtosis kurtosis-0.329
Angular range angular_range— – 0.2450 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha36490000.0000
Real-space data points n_real_points50
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.751; Stabil: 0.993; Sysdev: 1.000; Positv: 1.000; Valcen: 0.982; Smooth: 0.805
▶

5. Crystallography and Experiment 5. Crystallography & Experiment

▶

6. Entities and Polymers Entities & Polymers (2)

▼

7. Fold Classification (SCOP + CATH) 2 domains

CATH v4.4 (2 domains)

Domain ID domain_id4kmhA02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology1360 — Gyrase A; domain 2
Homologous superfamily homologous superfamily230 — Sufu, C-terminal domain
Domain ID domain_id4kmhB02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology1360 — Gyrase A; domain 2
Homologous superfamily homologous superfamily230 — Sufu, C-terminal domain
▶

8. Citations (1)

▶

9. Files and Curves (10)