1ml5

Structure of the E. coli ribosomal termination complex with release factor 2

Method: ELECTRON MICROSCOPY Dmax: 220.5 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

SAXS profile for 1ml5

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1ml5
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1ml5
沉积日期 deposition_date2002-08-30
结构标题 titleStructure of the E. coli ribosomal termination complex with release factor 2
关键词 keywordsE. coli, ribosome, termination of protein synthesis, release factor, cryo-eletron microscopy, angular reconstitution; RIBOSOME
实验方法 methodELECTRON MICROSCOPY

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier85.74
回转半径 Rg (电子) rg_electron87.19
零角强度 I(0) i017762700000.00
分子量 molecular_weight737850.0 kDa
排除体积 excluded_volume752140 ų
包络体积 envelope_volume2724300 ų
水化壳体积 shell_volume266880 ų
包络直径 envelope_diameter271.7
壳层 Rg shell_rg88.45
包络 Rg envelope_rg78.14
形状 Rg shape_rg81.05
总 Rg total_rg87.96
总原子数 total_atoms6303
残基数 n_residues4527
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax220.5
Rg (实空间) rg_real83.66
Rg 误差 (实空间) rg_real_error0.27
I(0) (实空间) i0_real1.6970e+10
I(0) 误差 (实空间) i0_real_error3.0240e+08
Rg (倒空间) rg_reciprocal87.35
I(0) (倒空间) i0_reciprocal17850000000.0000
解质量估计 total_estimate0.9009
解质量评级 solution_quality EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks1
主峰位置 r_peak_primary110.2
偏度 Skewness skewness0.066
峰度 Kurtosis kurtosis-0.578
角度范围 angular_range— – 0.0900 −1
当前正则化参数 α current_alpha1.3610
最高正则化参数 α highest_alpha4346000000.0000
实空间数据点数 n_real_points19
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 1.000; Stabil: 0.952; Sysdev: 1.000; Positv: 1.000; Valcen: 0.860; Smooth: 0.000

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (45)

6. 折叠分类 (SCOP + CATH) 22 domains

SCOP 2.08 (22 domains)

结构域编号 domain_idd1ml5c_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5e_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5f_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5g_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5h_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5i_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5j_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5k_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5l_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5m_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5n_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5o_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5p_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5q_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5r_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5s_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5t_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5u_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5v_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5w_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5x_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes
结构域编号 domain_idd1ml5z_
类 Class classi — Low resolution protein structures
折叠类型 Fold foldi.1 — Ribosome and ribosomal fragments
超家族 Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
家族 Family familyi.1.1.1 — Ribosome complexes

7. 引用文献 (1)

8. 文件与曲线 (10)