1ml5

Structure of the E. coli ribosomal termination complex with release factor 2

Method: ELECTRON MICROSCOPY

1. Protein Identity and Related Structures Protein Identity & Related Structures

Peptide chain release factor 2

Escherichia coli

UniProt P07012

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 40 RNA 5 30S 16S RIBOSOMAL RNA × 1 T-RNA(PHE) × 1 A- AND P-SITE MESSENGER RNA CODONS × 1 50S 23S RIBOSOMAL RNA × 1 50S 5S RIBOSOMAL RNA × 1 30S RIBOSOMAL PROTEIN S2 × 1 30S RIBOSOMAL PROTEIN S3 × 1 30S RIBOSOMAL PROTEIN S4 × 1 30S RIBOSOMAL PROTEIN S5 × 1 30S RIBOSOMAL PROTEIN S6 × 1 30S RIBOSOMAL PROTEIN S7 × 1 30S RIBOSOMAL PROTEIN S8 × 1 30S RIBOSOMAL PROTEIN S9 × 1 30S RIBOSOMAL PROTEIN S10 × 1 30S RIBOSOMAL PROTEIN S11 × 1 30S RIBOSOMAL PROTEIN S12 × 1 30S RIBOSOMAL PROTEIN S13 × 1 30S RIBOSOMAL PROTEIN S14 × 1 30S RIBOSOMAL PROTEIN S15 × 1 30S RIBOSOMAL PROTEIN S16 × 1 30S RIBOSOMAL PROTEIN S17 × 1 30S RIBOSOMAL PROTEIN S18 × 1 30S RIBOSOMAL PROTEIN S19 × 1 30S RIBOSOMAL PROTEIN S20 × 1 30S RIBOSOMAL PROTEIN THX × 1 50S RIBOSOMAL PROTEIN L1 × 1 50S RIBOSOMAL PROTEIN L2 × 1 50S RIBOSOMAL PROTEIN L3 × 1 50S RIBOSOMAL PROTEIN L4 × 1 50S RIBOSOMAL PROTEIN L5 × 1 50S RIBOSOMAL PROTEIN L6 × 1 50S RIBOSOMAL PROTEIN L11 × 1 50S RIBOSOMAL PROTEIN L13 × 1 50S RIBOSOMAL PROTEIN L14 × 1 50S RIBOSOMAL PROTEIN L15 × 1 50S RIBOSOMAL PROTEIN L16 × 1 50S RIBOSOMAL PROTEIN L18 × 1 50S RIBOSOMAL PROTEIN L19 × 1 50S RIBOSOMAL PROTEIN L22 × 1 50S RIBOSOMAL PROTEIN L23 × 1 50S RIBOSOMAL PROTEIN L24 × 1 50S RIBOSOMAL PROTEIN L25 × 1 50S RIBOSOMAL PROTEIN L29 × 1 50S RIBOSOMAL PROTEIN L30 × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name RF2_ECOLI
Isoform
PDB entities 6
Chains and sequence ranges Author chain Z; PDBConstruct 1–365; UniProt 1–365

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

2. Structure Basics 2. Structure Basics

Entry ID entry_id1ml5
Deposition date deposition_date2002-08-30
Structure title titleStructure of the E. coli ribosomal termination complex with release factor 2
Keywords keywordsE. coli, ribosome, termination of protein synthesis, release factor, cryo-eletron microscopy, angular reconstitution; RIBOSOME
Experimental Method methodELECTRON MICROSCOPY

3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1ml5__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1ml5__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 1010 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

1ml5__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg(Guinier)85.74 Å
Rg (electron density)87.19 Å
Total Rg87.96 Å
Atom count6303
Residues4599
Excluded volume752140 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 1ml5__assembly_1__model_1 45-meric (45) Success 4.1.3-1-20251215 (887e7ef) View Download

4. Crystallography and Experiment 4. Crystallography & Experiment

5. Entities and Polymers Entities & Polymers (45)

6. Fold Classification (SCOP + CATH) 22 domains

SCOP 2.08 (22 domains)

Domain ID domain_idd1ml5c_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5e_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5f_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5g_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5h_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5i_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5j_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5k_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5l_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5m_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5n_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5o_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5p_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5q_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5r_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5s_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5t_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5u_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5v_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5w_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5x_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes
Domain ID domain_idd1ml5z_
Class classi — Low resolution protein structures
Fold Fold foldi.1 — Ribosome and ribosomal fragments
Superfamily Superfamily superfamilyi.1.1 — Ribosome and ribosomal fragments
Family Family familyi.1.1.1 — Ribosome complexes

7. Citations (1)