1n12
;Crystal structure of the PapE (N-terminal-deleted) pilus subunit bound to a peptide corresponding to the N-terminal extension of the PapK pilus subunit (residues 1-11) from uropathogenic E. coli ;
SAXS 散射曲线 SAXS Profile
P(r) 距离分布 P(r) Distribution
1. 结构基本信息 1. Structure Basics
| 条目编号 entry_id | 1n12 |
| 沉积日期 deposition_date | 2002-10-16 |
| 结构标题 title | ;Crystal structure of the PapE (N-terminal-deleted) pilus subunit bound to a peptide corresponding to the N-terminal extension of the PapK pilus subunit (residues 1-11) from uropathogenic E. coli ; |
| 关键词 keywords | ;Immunoglobulin-like fold, donor strand complementation, donor strand exchange, chaperone priming, pilus fiber assembly, organelle biogenesis, CHAPERONE ;; CHAPERONE |
| 实验方法 method | X-RAY DIFFRACTION |
2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)
| 回转半径 Rg (Guinier) rg_guinier | 23.09 Å |
| 回转半径 Rg (电子) rg_electron | 22.12 Å |
| 零角强度 I(0) i0 | 18995400.00 |
| 分子量 molecular_weight | 31889.0 kDa |
| 排除体积 excluded_volume | 39301 ų |
| 包络体积 envelope_volume | 48618 ų |
| 水化壳体积 shell_volume | 18941 ų |
| 包络直径 envelope_diameter | 75.8 Å |
| 壳层 Rg shell_rg | 28.09 Å |
| 包络 Rg envelope_rg | 22.03 Å |
| 形状 Rg shape_rg | 22.09 Å |
| 总 Rg total_rg | 22.96 Å |
| 总原子数 total_atoms | 2218 |
| 残基数 n_residues | 290 |
| 球谐函数阶数 n_harmonics | 20 |
| q 范围 q_range | — – 0.5000 Å−1 |
| 数据点数 n_points | 101 |
| 壳层类型 shell_type | directional |
| 溶剂电子密度 solvent_density | 0.3340 e/ų |
| 壳层衬度 contrast_shell | 0.0300 e/ų |
| CRYSOL 版本 crysol_version | 4.1.3 |
3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)
| 最大尺寸 Dmax dmax | 74.5 Å |
| Rg (实空间) rg_real | 23.07 Å |
| Rg 误差 (实空间) rg_real_error | 0.55 Å |
| I(0) (实空间) i0_real | 1.9000e+07 |
| I(0) 误差 (实空间) i0_real_error | 2.5810e+05 |
| Rg (倒空间) rg_reciprocal | 23.08 Å |
| I(0) (倒空间) i0_reciprocal | 19000000.0000 |
| 解质量估计 total_estimate | 0.6669 |
| 解质量评级 solution_quality | REASONABLE a REASONABLE solution |
| P(r) 峰数 n_peaks | 2 |
| 主峰位置 r_peak_primary | 29.8 Å |
| 偏度 Skewness skewness | 0.237 |
| 峰度 Kurtosis kurtosis | -0.492 |
| 角度范围 angular_range | — – 0.3450 Å−1 |
| 当前正则化参数 α current_alpha | 0.0000 |
| 最高正则化参数 α highest_alpha | 3207000.0000 |
| 实空间数据点数 n_real_points | 66 |
| GNOM 版本 gnom_version | 4.1.3 |
| 质量判据 quality_criteria | AN1: 0.000; Oscil: 0.923; Stabil: 0.999; Sysdev: 0.307; Positv: 1.000; Valcen: 0.978; Smooth: 0.000 |
4. 晶体学与实验 4. Crystallography & Experiment
5. 实体与聚合体信息 (3)
6. 折叠分类 (SCOP + CATH) 4 domains
SCOP 2.08 (2 domains)
| 结构域编号 domain_id | d1n12a_ |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.2 — Common fold of diphtheria toxin/transcription factors/cytochrome f |
| 超家族 Superfamily superfamily | b.2.3 — Bacterial adhesins |
| 家族 Family family | b.2.3.2 — Pilus subunits |
| 结构域编号 domain_id | d1n12c_ |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.2 — Common fold of diphtheria toxin/transcription factors/cytochrome f |
| 超家族 Superfamily superfamily | b.2.3 — Bacterial adhesins |
| 家族 Family family | b.2.3.2 — Pilus subunits |
CATH v4.4 (2 domains)
| 结构域编号 domain_id | 1n12A00 |
| 类 Class class | 2 — Mainly Beta |
| 架构 Architecture architecture | 60 — Sandwich |
| 拓扑 Topology topology | 40 — Immunoglobulin-like |
| 同源超家族 H-superfamily homologous superfamily | 1090 — Fimbrial-type adhesion domain |
| 结构域编号 domain_id | 1n12C00 |
| 类 Class class | 2 — Mainly Beta |
| 架构 Architecture architecture | 60 — Sandwich |
| 拓扑 Topology topology | 40 — Immunoglobulin-like |
| 同源超家族 H-superfamily homologous superfamily | 1090 — Fimbrial-type adhesion domain |