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1niw

Crystal structure of endothelial nitric oxide synthase peptide bound to calmodulin

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

calmodulin

Rattus norvegicus

UniProt P62161

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 2 Nitric-oxide synthase, endothelial × 1 （P29474） CALCIUM ION × 4 SULFATE ION × 1 water × 2 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 2 Nitric-oxide synthase, endothelial × 1 （P29474） CALCIUM ION × 4 1,2-ETHANEDIOL × 2 water × 2 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 2 Nitric-oxide synthase, endothelial × 1 （P29474） CALCIUM ION × 4 1,2-ETHANEDIOL × 2 water × 2 Consistent with protein count
4 Protein heterocomplex Heteromer Protein 2 Nitric-oxide synthase, endothelial × 1 （P29474） CALCIUM ION × 4 water × 2 Consistent with protein count
5 Protein heterocomplex Heteromer Protein 8 Nitric-oxide synthase, endothelial × 4 （P29474） CALCIUM ION × 16 SULFATE ION × 1 1,2-ETHANEDIOL × 4 water × 8 Consistent with protein count
6 Protein heterocomplex Heteromer Protein 8 Nitric-oxide synthase, endothelial × 4 （P29474） CALCIUM ION × 16 SULFATE ION × 1 1,2-ETHANEDIOL × 4 water × 8 Consistent with protein count
7 Protein heterocomplex Heteromer Protein 4 Nitric-oxide synthase, endothelial × 2 （P29474） CALCIUM ION × 8 1,2-ETHANEDIOL × 2 water × 4 Consistent with protein count
8 Protein heterocomplex Heteromer Protein 4 Nitric-oxide synthase, endothelial × 2 （P29474） CALCIUM ION × 8 SULFATE ION × 1 1,2-ETHANEDIOL × 2 water × 4 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
2pq3 3b32 3ifk 3ifk 4rjd 4rjd

6 assemblies

Homodimer
1qx5 1qx5 1qx5 1qx5 1qx7 1qx7 2pq3 3b32 3b32

9 assemblies

Homotetramer

None

Other homooligomers
1qx7

1 assemblies

Heteromeric or nucleic-acid complex
1g4y 1niw 1niw 1niw 1niw 1niw 1niw 1niw 1niw 1qx7 2hqw 2mgu

32 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name CALM_RAT
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–148； UniProt 2–149 Author chain C； PDBConstruct 1–148； UniProt 2–149 Author chain E； PDBConstruct 1–148； UniProt 2–149 Author chain G； PDBConstruct 1–148； UniProt 2–149

Nitric-oxide synthase, endothelial

OrganismNot specified

UniProt P29474

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 2 calmodulin × 1 （P62161） CALCIUM ION × 4 SULFATE ION × 1 water × 2 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 2 calmodulin × 1 （P62161） CALCIUM ION × 4 1,2-ETHANEDIOL × 2 water × 2 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 2 calmodulin × 1 （P62161） CALCIUM ION × 4 1,2-ETHANEDIOL × 2 water × 2 Consistent with protein count
4 Protein heterocomplex Heteromer Protein 2 calmodulin × 1 （P62161） CALCIUM ION × 4 water × 2 Consistent with protein count
5 Protein heterocomplex Heteromer Protein 8 calmodulin × 4 （P62161） CALCIUM ION × 16 SULFATE ION × 1 1,2-ETHANEDIOL × 4 water × 8 Consistent with protein count
6 Protein heterocomplex Heteromer Protein 8 calmodulin × 4 （P62161） CALCIUM ION × 16 SULFATE ION × 1 1,2-ETHANEDIOL × 4 water × 8 Consistent with protein count
7 Protein heterocomplex Heteromer Protein 4 calmodulin × 2 （P62161） CALCIUM ION × 8 1,2-ETHANEDIOL × 2 water × 4 Consistent with protein count
8 Protein heterocomplex Heteromer Protein 4 calmodulin × 2 （P62161） CALCIUM ION × 8 SULFATE ION × 1 1,2-ETHANEDIOL × 2 water × 4 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer
1m9j 1m9k 1m9m 1m9q 1m9r 3eah 3nos 4d1o 4d1p 5uo8 5uo8 5uo9

170 assemblies; showing the first 12

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1niw 1niw 1niw 1niw 1niw 1niw 1niw 1niw 2ll7 2n8j 5xof 5xof

14 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name NOS3_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–20； UniProt 492–511 Author chain D； PDBConstruct 1–20； UniProt 492–511 Author chain F； PDBConstruct 1–20； UniProt 492–511 Author chain H； PDBConstruct 1–20； UniProt 492–511

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id1niw
Deposition date deposition_date2002-12-26
Structure title titleCrystal structure of endothelial nitric oxide synthase peptide bound to calmodulin
Keywords keywordsNitric oxide, calcium-binding protein, NOS, SIGNALING PROTEIN-OXIDOREDUCTASE COMPLEX; SIGNALING PROTEIN/OXIDOREDUCTASE
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1niw__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1niw__assembly_1__model_1 | I(q)

10-2 10-1 105 106 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

1niw__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）16.94 Å
Rg (electron density)15.75 Å
Total Rg16.49 Å
Atom count1299
Residues155
Excluded volume23092 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 1niw__assembly_1__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 1niw__assembly_2__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
3 1 1niw__assembly_3__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
4 1 1niw__assembly_4__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
5 1 1niw__assembly_5__model_1 octameric （8） Success 4.1.3-1-20251215 (887e7ef) View Download
6 1 1niw__assembly_6__model_1 octameric （8） Success 4.1.3-1-20251215 (887e7ef) View Download
7 1 1niw__assembly_7__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
8 1 1niw__assembly_8__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (6)

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6. Fold Classification (SCOP + CATH) 12 domains

SCOP 2.08 (4 domains)

Domain ID domain_idd1niwa_
Class classa — All alpha proteins
Fold Fold folda.39 — EF Hand-like
Superfamily Superfamily superfamilya.39.1 — EF-hand
Family Family familya.39.1.5 — Calmodulin-like
Domain ID domain_idd1niwc_
Class classa — All alpha proteins
Fold Fold folda.39 — EF Hand-like
Superfamily Superfamily superfamilya.39.1 — EF-hand
Family Family familya.39.1.5 — Calmodulin-like
Domain ID domain_idd1niwe_
Class classa — All alpha proteins
Fold Fold folda.39 — EF Hand-like
Superfamily Superfamily superfamilya.39.1 — EF-hand
Family Family familya.39.1.5 — Calmodulin-like
Domain ID domain_idd1niwg_
Class classa — All alpha proteins
Fold Fold folda.39 — EF Hand-like
Superfamily Superfamily superfamilya.39.1 — EF-hand
Family Family familya.39.1.5 — Calmodulin-like

CATH v4.4 (8 domains)

Domain ID domain_id1niwA01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
Domain ID domain_id1niwA02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
Domain ID domain_id1niwC01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
Domain ID domain_id1niwC02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
Domain ID domain_id1niwE01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
Domain ID domain_id1niwE02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
Domain ID domain_id1niwG01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
Domain ID domain_id1niwG02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
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7. Citations (1)