1nt0

Crystal structure of the CUB1-EGF-CUB2 region of MASP2

Method: X-RAY DIFFRACTION Dmax: 131.0 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 1nt0

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1nt0
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1nt0
沉积日期 deposition_date2003-01-28
结构标题 titleCrystal structure of the CUB1-EGF-CUB2 region of MASP2
关键词 keywordsmannose-binding protein, MASP, CUB domain, EGF like domain., HYDROLASE, SUGAR BINDING PROTEIN; HYDROLASE, SUGAR BINDING PROTEIN
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier36.86
回转半径 Rg (电子) rg_electron37.54
零角强度 I(0) i054758500.00
分子量 molecular_weight58019.0 kDa
排除体积 excluded_volume71972 ų
包络体积 envelope_volume101090 ų
水化壳体积 shell_volume25621 ų
包络直径 envelope_diameter140.8
壳层 Rg shell_rg37.48
包络 Rg envelope_rg37.57
形状 Rg shape_rg37.56
总 Rg total_rg37.49
总原子数 total_atoms4088
残基数 n_residues512
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax131.0
Rg (实空间) rg_real37.47
Rg 误差 (实空间) rg_real_error1.73
I(0) (实空间) i0_real5.4760e+07
I(0) 误差 (实空间) i0_real_error9.6530e+05
Rg (倒空间) rg_reciprocal37.09
I(0) (倒空间) i0_reciprocal54740000.0000
解质量估计 total_estimate0.7596
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks3
主峰位置 r_peak_primary42.6
偏度 Skewness skewness0.613
峰度 Kurtosis kurtosis-0.094
角度范围 angular_range— – 0.2150 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha2722000.0000
实空间数据点数 n_real_points44
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.671; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.405; Smooth: 0.451

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (5)

6. 折叠分类 (SCOP + CATH) 12 domains

SCOP 2.08 (6 domains)

结构域编号 domain_idd1nt0a1
类 Class classb — All beta proteins
折叠类型 Fold foldb.23 — CUB-like
超家族 Superfamily superfamilyb.23.1 — Spermadhesin, CUB domain
家族 Family familyb.23.1.1 — Spermadhesin, CUB domain
结构域编号 domain_idd1nt0a2
类 Class classb — All beta proteins
折叠类型 Fold foldb.23 — CUB-like
超家族 Superfamily superfamilyb.23.1 — Spermadhesin, CUB domain
家族 Family familyb.23.1.1 — Spermadhesin, CUB domain
结构域编号 domain_idd1nt0a3
类 Class classg — Small proteins
折叠类型 Fold foldg.3 — Knottins (small inhibitors, toxins, lectins)
超家族 Superfamily superfamilyg.3.11 — EGF/Laminin
家族 Family familyg.3.11.1 — EGF-type module
结构域编号 domain_idd1nt0g1
类 Class classb — All beta proteins
折叠类型 Fold foldb.23 — CUB-like
超家族 Superfamily superfamilyb.23.1 — Spermadhesin, CUB domain
家族 Family familyb.23.1.1 — Spermadhesin, CUB domain
结构域编号 domain_idd1nt0g2
类 Class classb — All beta proteins
折叠类型 Fold foldb.23 — CUB-like
超家族 Superfamily superfamilyb.23.1 — Spermadhesin, CUB domain
家族 Family familyb.23.1.1 — Spermadhesin, CUB domain
结构域编号 domain_idd1nt0g3
类 Class classg — Small proteins
折叠类型 Fold foldg.3 — Knottins (small inhibitors, toxins, lectins)
超家族 Superfamily superfamilyg.3.11 — EGF/Laminin
家族 Family familyg.3.11.1 — EGF-type module

CATH v4.4 (6 domains)

结构域编号 domain_id1nt0A01
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily290 — Spermadhesin, CUB domain
结构域编号 domain_id1nt0A02
类 Class class2 — Mainly Beta
架构 Architecture architecture10 — Ribbon
拓扑 Topology topology25 — Laminin
同源超家族 H-superfamily homologous superfamily10 — Laminin
结构域编号 domain_id1nt0A03
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily290 — Spermadhesin, CUB domain
结构域编号 domain_id1nt0G01
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily290 — Spermadhesin, CUB domain
结构域编号 domain_id1nt0G02
类 Class class2 — Mainly Beta
架构 Architecture architecture10 — Ribbon
拓扑 Topology topology25 — Laminin
同源超家族 H-superfamily homologous superfamily10 — Laminin
结构域编号 domain_id1nt0G03
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily290 — Spermadhesin, CUB domain

7. 引用文献 (1)

8. 文件与曲线 (10)