1o5z

Crystal structure of Folylpolyglutamate synthase (TM0166) from Thermotoga maritima at 2.10 A resolution

Method: X-RAY DIFFRACTION

SAXS 散射曲线 SAXS Profile

暂无 SAXS 图

P(r) 距离分布 P(r) Distribution

暂无 P(r) 图

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	1o5z
沉积日期 deposition_date	2003-10-10
结构标题 title	Crystal structure of Folylpolyglutamate synthase (TM0166) from Thermotoga maritima at 2.10 A resolution
关键词 keywords	;TM0166, FOLYLPOLYGLUTAMATE SYNTHASE, STRUCTURAL GENOMICS, JCSG, PSI, Protein Structure Initiative, Joint Center for Structural Genomics, LIGASE ;; LIGASE
实验方法 method	X-RAY DIFFRACTION

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

该条目暂无 SAXS 数据。

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

该条目暂无 P(r) 分析数据。

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (5)

Entity 1 polymer

描述 description	folylpolyglutamate synthase/dihydrofolate synthase
分子量 formula_weight	50399.0 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A
原子数 atom_count	3716

MGSDKIHHHHHHMAYLEVLRYLYHKRPMGKVKPGLERISMLLSKLGNPHLEYKTIHIGGTNGKGSVANMVSNILVSQGYRVGSYYSPHLSTFRERIRLNEEYISEEDVVKIYETMEPILNELDKEEIFSPSFFEVVTAMAFLYFAEKNVDIAVLEVGLGGRLDATNVVFPLCSTIVTVDRDHEKTLGYTIEQIAWEKSGIIKERVPLVTGERKREALKVMEDVARKKSSRMYVIDKDFSVKVKSLKLHENRFDYCGENTFEDLVLTMNGPHQIENAGVALKTLEATGLPLSEKAIREGLKNAKNLGRFEILEKNGKMYILDGAHNPHGAESLVRSLKLYFNGEPLSLVIGILDDKNREDILRKYTGIFERVIVTRVPSPRMKDMNSLVDMAKKFFKNVEVIEDPLEAIESTERATVVTGSLFLVGYVREFLTTGKINEEWKL

来源 Source

来源生物 organism	Thermotoga maritima
NCBI 分类编号 ncbi_taxonomy_id	2336
宿主生物 host_organism	Escherichia coli

Entity 2 non-polymer

描述 description	SULFATE ION
分子量 formula_weight	96.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	4

Entity 3 non-polymer

描述 description	CHLORIDE ION
分子量 formula_weight	35.5 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

Entity 4 non-polymer

描述 description	UNKNOWN LIGAND
来源方法 src_method	syn
拷贝数 entity_count	2

Entity 5 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	323

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6. 折叠分类 (SCOP + CATH) 5 domains

SCOP 2.08 (3 domains)

结构域编号 domain_id	d1o5za1
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.59 — MurD-like peptide ligases, peptide-binding domain
超家族 Superfamily superfamily	c.59.1 — MurD-like peptide ligases, peptide-binding domain
家族 Family family	c.59.1.2 — Folylpolyglutamate synthetase, C-terminal domain

结构域编号 domain_id	d1o5za2
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.72 — Ribokinase-like
超家族 Superfamily superfamily	c.72.2 — MurD-like peptide ligases, catalytic domain
家族 Family family	c.72.2.2 — Folylpolyglutamate synthetase

结构域编号 domain_id	d1o5za3
类 Class class	l — Artifacts
折叠类型 Fold fold	l.1 — Tags
超家族 Superfamily superfamily	l.1.1 — Tags
家族 Family family	l.1.1.1 — Tags

CATH v4.4 (2 domains)

结构域编号 domain_id	1o5zA01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	1190 — UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase
同源超家族 H-superfamily homologous superfamily	10 — Mur-like, catalytic domain

结构域编号 domain_id	1o5zA02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	90 — Alpha-Beta Complex
拓扑 Topology topology	190 — Protein-Tyrosine Phosphatase; Chain A
同源超家族 H-superfamily homologous superfamily	20 — Mur ligase, C-terminal domain

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7. 引用文献 (1)

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晶胞边长 a length_a	84.751 Å
晶胞边长 b length_b	84.751 Å
晶胞边长 c length_c	280.679 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	120.00 °
晶胞分子数 Z z_pdb	12

空间群 space_group	P 61 2 2
空间群编号 int_tables_number	178
晶系 crystal_system	—

分辨率 resolution	2.100 Å
最低分辨率 resolution_low	35.190 Å
R 因子 r_factor	0.1842
R_work r_work	0.1811
R_free r_free	0.2442