1ozh

The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate.

Method: X-RAY DIFFRACTION Dmax: 118.0 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

SAXS profile for 1ozh

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1ozh
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1ozh
沉积日期 deposition_date2003-04-09
结构标题 titleThe crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate.
关键词 keywordsacetolactate synthase, acetohydroxyacid synthase, thiamin diphosphate, LYASE; LYASE
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier38.65
回转半径 Rg (电子) rg_electron37.79
零角强度 I(0) i0826921000.00
分子量 molecular_weight234140.0 kDa
排除体积 excluded_volume292460 ų
包络体积 envelope_volume356840 ų
水化壳体积 shell_volume74823 ų
包络直径 envelope_diameter119.1
壳层 Rg shell_rg46.63
包络 Rg envelope_rg37.30
形状 Rg shape_rg37.81
总 Rg total_rg38.16
总原子数 total_atoms16455
残基数 n_residues2171
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax118.0
Rg (实空间) rg_real38.36
Rg 误差 (实空间) rg_real_error0.52
I(0) (实空间) i0_real8.2690e+08
I(0) 误差 (实空间) i0_real_error1.2200e+07
Rg (倒空间) rg_reciprocal38.55
I(0) (倒空间) i0_reciprocal827100000.0000
解质量估计 total_estimate0.9041
解质量评级 solution_quality EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks1
主峰位置 r_peak_primary48.9
偏度 Skewness skewness0.083
峰度 Kurtosis kurtosis-0.606
角度范围 angular_range— – 0.2050 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha239900000.0000
实空间数据点数 n_real_points42
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.940; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.973; Smooth: 0.957

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (7)

6. 折叠分类 (SCOP + CATH) 28 domains

SCOP 2.08 (15 domains)

结构域编号 domain_idd1ozha1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.31 — DHS-like NAD/FAD-binding domain
超家族 Superfamily superfamilyc.31.1 — DHS-like NAD/FAD-binding domain
家族 Family familyc.31.1.3 — Pyruvate oxidase and decarboxylase, middle domain
结构域编号 domain_idd1ozha2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.36 — Thiamin diphosphate-binding fold (THDP-binding)
超家族 Superfamily superfamilyc.36.1 — Thiamin diphosphate-binding fold (THDP-binding)
家族 Family familyc.36.1.5 — Pyruvate oxidase and decarboxylase Pyr module
结构域编号 domain_idd1ozha3
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.36 — Thiamin diphosphate-binding fold (THDP-binding)
超家族 Superfamily superfamilyc.36.1 — Thiamin diphosphate-binding fold (THDP-binding)
家族 Family familyc.36.1.9 — Pyruvate oxidase and decarboxylase PP module
结构域编号 domain_idd1ozha4
类 Class classl — Artifacts
折叠类型 Fold foldl.1 — Tags
超家族 Superfamily superfamilyl.1.1 — Tags
家族 Family familyl.1.1.1 — Tags
结构域编号 domain_idd1ozhb1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.31 — DHS-like NAD/FAD-binding domain
超家族 Superfamily superfamilyc.31.1 — DHS-like NAD/FAD-binding domain
家族 Family familyc.31.1.3 — Pyruvate oxidase and decarboxylase, middle domain
结构域编号 domain_idd1ozhb2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.36 — Thiamin diphosphate-binding fold (THDP-binding)
超家族 Superfamily superfamilyc.36.1 — Thiamin diphosphate-binding fold (THDP-binding)
家族 Family familyc.36.1.5 — Pyruvate oxidase and decarboxylase Pyr module
结构域编号 domain_idd1ozhb3
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.36 — Thiamin diphosphate-binding fold (THDP-binding)
超家族 Superfamily superfamilyc.36.1 — Thiamin diphosphate-binding fold (THDP-binding)
家族 Family familyc.36.1.9 — Pyruvate oxidase and decarboxylase PP module
结构域编号 domain_idd1ozhb4
类 Class classl — Artifacts
折叠类型 Fold foldl.1 — Tags
超家族 Superfamily superfamilyl.1.1 — Tags
家族 Family familyl.1.1.1 — Tags
结构域编号 domain_idd1ozhc1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.31 — DHS-like NAD/FAD-binding domain
超家族 Superfamily superfamilyc.31.1 — DHS-like NAD/FAD-binding domain
家族 Family familyc.31.1.3 — Pyruvate oxidase and decarboxylase, middle domain
结构域编号 domain_idd1ozhc2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.36 — Thiamin diphosphate-binding fold (THDP-binding)
超家族 Superfamily superfamilyc.36.1 — Thiamin diphosphate-binding fold (THDP-binding)
家族 Family familyc.36.1.5 — Pyruvate oxidase and decarboxylase Pyr module
结构域编号 domain_idd1ozhc3
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.36 — Thiamin diphosphate-binding fold (THDP-binding)
超家族 Superfamily superfamilyc.36.1 — Thiamin diphosphate-binding fold (THDP-binding)
家族 Family familyc.36.1.9 — Pyruvate oxidase and decarboxylase PP module
结构域编号 domain_idd1ozhc4
类 Class classl — Artifacts
折叠类型 Fold foldl.1 — Tags
超家族 Superfamily superfamilyl.1.1 — Tags
家族 Family familyl.1.1.1 — Tags
结构域编号 domain_idd1ozhd1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.31 — DHS-like NAD/FAD-binding domain
超家族 Superfamily superfamilyc.31.1 — DHS-like NAD/FAD-binding domain
家族 Family familyc.31.1.3 — Pyruvate oxidase and decarboxylase, middle domain
结构域编号 domain_idd1ozhd2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.36 — Thiamin diphosphate-binding fold (THDP-binding)
超家族 Superfamily superfamilyc.36.1 — Thiamin diphosphate-binding fold (THDP-binding)
家族 Family familyc.36.1.5 — Pyruvate oxidase and decarboxylase Pyr module
结构域编号 domain_idd1ozhd3
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.36 — Thiamin diphosphate-binding fold (THDP-binding)
超家族 Superfamily superfamilyc.36.1 — Thiamin diphosphate-binding fold (THDP-binding)
家族 Family familyc.36.1.9 — Pyruvate oxidase and decarboxylase PP module

CATH v4.4 (13 domains)

结构域编号 domain_id1ozhA01
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily970 — Thiamin diphosphate (ThDP)-binding fold, Pyr/PP domains
结构域编号 domain_id1ozhA02
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily1220 — TPP-binding domain
结构域编号 domain_id1ozhA03
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily740 — Single helix bin
结构域编号 domain_id1ozhA04
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily970 — Thiamin diphosphate (ThDP)-binding fold, Pyr/PP domains
结构域编号 domain_id1ozhB01
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily970 — Thiamin diphosphate (ThDP)-binding fold, Pyr/PP domains
结构域编号 domain_id1ozhB02
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily1220 — TPP-binding domain
结构域编号 domain_id1ozhB03
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily970 — Thiamin diphosphate (ThDP)-binding fold, Pyr/PP domains
结构域编号 domain_id1ozhC01
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily970 — Thiamin diphosphate (ThDP)-binding fold, Pyr/PP domains
结构域编号 domain_id1ozhC02
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily1220 — TPP-binding domain
结构域编号 domain_id1ozhC03
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily970 — Thiamin diphosphate (ThDP)-binding fold, Pyr/PP domains
结构域编号 domain_id1ozhD01
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily970 — Thiamin diphosphate (ThDP)-binding fold, Pyr/PP domains
结构域编号 domain_id1ozhD02
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily1220 — TPP-binding domain
结构域编号 domain_id1ozhD03
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily970 — Thiamin diphosphate (ThDP)-binding fold, Pyr/PP domains

7. 引用文献 (1)

8. 文件与曲线 (10)