1piu

OXIDIZED RIBONUCLEOTIDE REDUCTASE R2-D84E MUTANT CONTAINING OXO-BRIDGED DIFERRIC CLUSTER

Method: X-RAY DIFFRACTION

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

Ribonucleoside-diphosphate reductase 1 beta chain

Escherichia coli

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白同源多聚体同源多聚体蛋白 × 2 PDB 声明：dimeric（2）与蛋白拷贝数一致	链 A； UniProt 1–375 链 B； UniProt 1–375	突变：D84E	FE FE (III) ION × 4 HG MERCURY (II) ION × 10 O OXYGEN ATOM × 2	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 6；310 K；20% PEG 4000, 200mM NaCl, 50mM MES, 0.3% dioxane, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 310K	分辨率 2.20 Å R-free 0.232

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly， “相对当前条目”直接指出证据层面的不同；没有差异标签表示当前已读取字段一致。

其他 PDB	相对当前条目 1PIU	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
1AV8 RIBONUCLEOTIDE REDUCTASE R2 SUBUNIT FROM E. COLI 提交 1997-09-30	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–340（340 aa）链 B 1–340（340 aa）	未记录	FEO MU-OXO-DIIRON × 2	X-RAY DIFFRACTION X-ray结晶条件 pH 7.6；PROTEIN WAS CRYSTALLIZED FROM 80% SATURATED NACL, NEAR PHYSIOLOGICAL PH (PH 7.6)	分辨率 2.80 Å R-free 0.192
1BIQ RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE 1 BETA CHAIN MUTANT E238A 提交 1998-06-18	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–375（375 aa）片段：BETA CHAIN 链 B 1–375（375 aa）片段：BETA CHAIN	突变：Y122F, E238A 非标准单体：是（mmCIF未提供具体位点）突变：Y122F, E238A	FE2 FE (II) ION × 3 FE FE (III) ION × 1 OH HYDROXIDE ION × 3 HG MERCURY (II) ION × 16	X-RAY DIFFRACTION X-ray结晶条件 pH 6；pH 6.0	分辨率 2.05 Å R-free 0.260
1JPR Mn substituted Ribonucleotide reductase R2 from E. coli oxidized by nitric oxide 提交 2001-08-03	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–375（375 aa）链 B 1–375（375 aa）	未记录	MN MANGANESE (II) ION × 4 HG MERCURY (II) ION × 14	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；PEG 4000, sodium chloride, EMTS, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 1.88 Å R-free 0.213
1JQC Mn substituted Ribonucleotide reductase R2 from E. Coli oxidized by hydrogen peroxide and hydroxylamine 提交 2001-08-06	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–375（375 aa）链 B 1–375（375 aa）	未记录	MN MANGANESE (II) ION × 4 HG MERCURY (II) ION × 13	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；peg 4000, MES, EMTS, sodium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 1.61 Å R-free 0.219
1MRR SUBSTITUTION OF MANGANESE FOR IRON IN RIBONUCLEOTIDE REDUCTASE FROM ESCHERICHIA COLI. SPECTROSCOPIC AND CRYSTALLOGRAPHIC CHARACTERIZATION 提交 1992-07-28	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–375（375 aa）链 B 1–375（375 aa）	未记录	MN MANGANESE (II) ION × 4 HG MERCURY (II) ION × 13	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1MXR High resolution structure of Ribonucleotide reductase R2 from E. coli in its oxidised (Met) form 提交 2002-10-03	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–375（375 aa）链 B 1–375（375 aa）	未记录	FE FE (III) ION × 4 HG MERCURY (II) ION × 11 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；PEG 4000, MES, NaCl, EMTS, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 1.42 Å R-free 0.185
1PFR RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE 1 BETA CHAIN 提交 1996-12-03	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–340（340 aa）链 B 1–340（340 aa）	突变：S211A 突变：S211A	FE FE (III) ION × 4 HG MERCURY (II) ION × 8	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6；HANGING DROP VAPOR DIFFUSION. CRYSTALLISED FROM 20% PEG 4000, 0.2M NACL, 0.1M MES PH 6.0, 1MM ETHYLMERCURY SALICYLATE., vapor diffusion - hanging drop	分辨率 2.20 Å
1PIM DITHIONITE REDUCED E. COLI RIBONUCLEOTIDE REDUCTASE R2 SUBUNIT, D84E MUTANT 提交 2003-05-30	配体/离子不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–375（375 aa）链 B 1–375（375 aa）	突变：D84E 突变：D84E	FE FE (III) ION × 4 HG MERCURY (II) ION × 7	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6；310 K；20% PEG 4000, 200mM NaCl, 50mM MES, 0.3% dioxane, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 310K	分辨率 2.00 Å R-free 0.246
1PIY RIBONUCLEOTIDE REDUCTASE R2 SOAKED WITH FERROUS ION AT NEUTRAL PH 提交 2003-05-30	突变/修饰不同配体/离子不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–375（375 aa）链 B 1–375（375 aa）	未记录	FE FE (III) ION × 4 HG MERCURY (II) ION × 14	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6；310 K；20% PEG 4000, 200mM NaCl, 50mM MES, 0.3% dioxane, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 310K	分辨率 1.68 Å R-free 0.246
1PIZ RIBONUCLEOTIDE REDUCTASE R2 D84E MUTANT SOAKED WITH FERROUS IONS AT NEUTRAL PH 提交 2003-05-30	配体/离子不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–375（375 aa）链 B 1–375（375 aa）	突变：D84E 突变：D84E	FE FE (III) ION × 4 HG MERCURY (II) ION × 11	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6；310 K；20% PEG 4000, 200mM NaCl, 50mM MES, 0.3% dioxane, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 310K	分辨率 1.90 Å R-free 0.242
1PJ0 RIBONUCLEOTIDE REDUCTASE R2-D84E/W48F MUTANT SOAKED WITH FERROUS IONS AT NEUTRAL PH 提交 2003-05-30	突变/修饰不同配体/离子不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–375（375 aa）链 B 1–375（375 aa）	突变：D84E, W48F 突变：D84E, W48F	FE FE (III) ION × 4 HG MERCURY (II) ION × 9	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6；310 K；20% PEG 4000, 200mM NaCl, 50mM MES, 0.3% dioxane, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 310K	分辨率 1.90 Å R-free 0.236
1PJ1 RIBONUCLEOTIDE REDUCTASE R2-D84E/W48F SOAKED WITH FERROUS IONS AT PH 5 提交 2003-05-30	突变/修饰不同配体/离子不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–375（375 aa）链 B 1–375（375 aa）	突变：D84E, W48F 突变：D84E, W48F	FE FE (III) ION × 4 HG MERCURY (II) ION × 15	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6；310 K；20% PEG 4000, 200mM NaCl, 50mM MES, 0.3% dioxane, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 310K	分辨率 1.95 Å R-free 0.221
1PM2 CRYSTAL STRUCTURE OF MANGANESE SUBSTITUTED R2-D84E (D84E MUTANT OF THE R2 SUBUNIT OF E. COLI RIBONUCLEOTIDE REDUCTASE) 提交 2003-06-09	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–339（339 aa）链 B 1–339（339 aa）	突变：D84E 突变：D84E	MN MANGANESE (II) ION × 4 HG MERCURY (II) ION × 14	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.3；310 K；PEG 4000, MES, sodium chloride, sodium ethylmercurithiosalicylicate , pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 310K	分辨率 1.80 Å R-free 0.215
1R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN MUTANT Y730F WITH A REDUCED ACTIVE SITE FROM ESCHERICHIA COLI 提交 1997-07-15	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 3 PDB 声明：trimeric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 P 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 2.90 Å R-free 0.245
1R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN MUTANT Y730F WITH A REDUCED ACTIVE SITE FROM ESCHERICHIA COLI 提交 1997-07-15	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 E 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 2.90 Å R-free 0.245
1R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN MUTANT Y730F WITH A REDUCED ACTIVE SITE FROM ESCHERICHIA COLI 提交 1997-07-15	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 F 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 2.90 Å R-free 0.245
1R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN MUTANT Y730F WITH A REDUCED ACTIVE SITE FROM ESCHERICHIA COLI 提交 1997-07-15	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 4 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 E 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 2.90 Å R-free 0.245
1R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN MUTANT Y730F WITH A REDUCED ACTIVE SITE FROM ESCHERICHIA COLI 提交 1997-07-15	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 5 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 F 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 2.90 Å R-free 0.245
1R65 Crystal structure of ferrous soaked Ribonucleotide Reductase R2 subunit (wildtype) at pH 5 from E. coli 提交 2003-10-14	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–375（375 aa）片段：Ribonucleotide Reductase R2 链 B 1–375（375 aa）片段：Ribonucleotide Reductase R2	未记录	FE2 FE (II) ION × 4 HG MERCURY (II) ION × 13	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6；310 K；PEG4000, sodium chloride, EMTS, MES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 310K	分辨率 1.95 Å R-free 0.236
1RIB STRUCTURE AND FUNCTION OF THE ESCHERICHIA COLI RIBONUCLEOTIDE REDUCTASE PROTEIN R2 提交 1993-01-19	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–375（375 aa）链 B 1–375（375 aa）	未记录	FEO MU-OXO-DIIRON × 2	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.20 Å
1RNR AUTOCATALYTIC GENERATION OF DOPA IN THE ENGINEERED PROTEIN R2 F208Y FROM ESCHERICHIA COLI RIBONUCLEOTIDE REDUCTASE AND CRYSTAL STRUCTURE OF THE DOPA-208 PROTEIN 提交 1993-04-26	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 2–376（375 aa）链 B 2–376（375 aa）	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	FE FE (III) ION × 4 HG MERCURY (II) ION × 14	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1RSR azide complex of the diferrous F208A mutant R2 subunit of ribonucleotide reductase 提交 2003-12-10	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–375（375 aa）链 B 1–375（375 aa）	突变：Y122F, F208A 突变：Y122F, F208A	FE2 FE (II) ION × 4 HG MERCURY (II) ION × 12 AZI AZIDE ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；100mM MES, 200mM NaCl, 1mM EMTS (Thimerosal), 16-24% PEG 4000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.00 Å R-free 0.289
1RSV azide complex of the diferrous E238A mutant R2 subunit of ribonucleotide reductase 提交 2003-12-10	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–375（375 aa）链 B 1–375（375 aa）	突变：Y122F, E238A 突变：Y122F, E238A	FE FE (III) ION × 4 HG MERCURY (II) ION × 12 AZI AZIDE ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；100mM MES, 200mM NaCl, 1mM EMTS (Thimerosal), 16-24% PEG 4000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.20 Å R-free 0.256
1XIK RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE 1 BETA CHAIN 提交 1996-08-06	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–375（375 aa）片段：BETA CHAIN 链 B 1–375（375 aa）片段：BETA CHAIN	未记录	FE2 FE (II) ION × 4 HG MERCURY (II) ION × 10	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6；HANGING DROP VAPOR DIFFUSION METHOD, 20% PEG 4000, 0.2M NACL, 50MM MES BUFFER PH 6.0, 1MM ETHYLMERCURY SALICYLATE, vapor diffusion - hanging drop	分辨率 1.70 Å R-free 0.280
1YFD Crystal structure of the Y122H mutant of ribonucleotide reductase R2 protein from E. coli 提交 2004-12-31	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–375（375 aa）链 B 1–375（375 aa）	突变：Y122H 突变：Y122H	HG MERCURY (II) ION × 13 FEO MU-OXO-DIIRON × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6；295 K；PEG 4000, 0.1M MES, 0.4M NACL, 1mM EMTS, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K	分辨率 1.90 Å R-free 0.206
2ALX Ribonucleotide Reductase R2 from Escherichia coli in space group P6(1)22 提交 2005-08-08	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 0–339（340 aa）片段：residues 0-339	未记录	MN MANGANESE (II) ION × 4 HG MERCURY (II) ION × 8	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；ammonium sulfate, MES, manganese chloride, magnesium chloride, 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid, ethylmercurithiosalicylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.60 Å R-free 0.276
2AV8 Y122F MUTANT OF RIBONUCLEOTIDE REDUCTASE FROM ESCHERICHIA COLI 提交 1997-09-30	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–340（340 aa）链 B 1–340（340 aa）	突变：Y122F 突变：Y122F	FE2 FE (II) ION × 1 FEO MU-OXO-DIIRON × 2	X-RAY DIFFRACTION X-ray结晶条件 pH 7.6；PROTEIN WAS CRYSTALLIZED FROM 80% SATURATED NACL, NEAR PHYSIOLOGICAL PH (PH 7.6)	分辨率 2.46 Å R-free 0.237
2R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH DTTP OCCUPYING THE SPECIFICITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 3 PDB 声明：trimeric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 P 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	TTP THYMIDINE-5'-TRIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION	分辨率 3.00 Å R-free 0.280
2R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH DTTP OCCUPYING THE SPECIFICITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 E 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	TTP THYMIDINE-5'-TRIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION	分辨率 3.00 Å R-free 0.280
2R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH DTTP OCCUPYING THE SPECIFICITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 F 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	TTP THYMIDINE-5'-TRIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION	分辨率 3.00 Å R-free 0.280
2R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH DTTP OCCUPYING THE SPECIFICITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 4 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 E 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	TTP THYMIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION	分辨率 3.00 Å R-free 0.280
2R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH DTTP OCCUPYING THE SPECIFICITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 5 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 F 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	TTP THYMIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION	分辨率 3.00 Å R-free 0.280
2R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH DTTP OCCUPYING THE SPECIFICITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 6 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 E 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	TTP THYMIDINE-5'-TRIPHOSPHATE × 2	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION	分辨率 3.00 Å R-free 0.280
2R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH DTTP OCCUPYING THE SPECIFICITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 7 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 F 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	TTP THYMIDINE-5'-TRIPHOSPHATE × 2	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION	分辨率 3.00 Å R-free 0.280
2R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH DTTP OCCUPYING THE SPECIFICITY SITE FROM ESCHERICHIA COLI 提交 1997-07-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 8 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	TTP THYMIDINE-5'-TRIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM CDP WAS ALSO INCLUDED IN THE PROTEIN SOLUTION	分辨率 3.00 Å R-free 0.280
2X0X Ribonucleotide reductase R1 subunit of E. coli to 2.3 A resolution 提交 2009-12-18	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 F 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	SO4 SULFATE ION × 6	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE APP 1.5M, SODIUM CITRATE BUFFER PH APP 6.	分辨率 2.30 Å R-free 0.224
2X0X Ribonucleotide reductase R1 subunit of E. coli to 2.3 A resolution 提交 2009-12-18	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 D 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376 链 E 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	SO4 SULFATE ION × 6	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE APP 1.5M, SODIUM CITRATE BUFFER PH APP 6.	分辨率 2.30 Å R-free 0.224
2X0X Ribonucleotide reductase R1 subunit of E. coli to 2.3 A resolution 提交 2009-12-18	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 P 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE APP 1.5M, SODIUM CITRATE BUFFER PH APP 6.	分辨率 2.30 Å R-free 0.224
2XAK Ribonucleotide reductase Y730NO2Y modified R1 subunit of E. coli 提交 2010-03-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 D 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376 链 E 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.80 Å R-free 0.241
2XAK Ribonucleotide reductase Y730NO2Y modified R1 subunit of E. coli 提交 2010-03-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 F 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.80 Å R-free 0.241
2XAK Ribonucleotide reductase Y730NO2Y modified R1 subunit of E. coli 提交 2010-03-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 P 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.80 Å R-free 0.241
2XAP Ribonucleotide reductase Y731NO2Y modified R1 subunit of E. coli to 2. 1 A resolution 提交 2010-03-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 D 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376 链 E 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.10 Å R-free 0.230
2XAP Ribonucleotide reductase Y731NO2Y modified R1 subunit of E. coli to 2. 1 A resolution 提交 2010-03-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 F 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.10 Å R-free 0.230
2XAP Ribonucleotide reductase Y731NO2Y modified R1 subunit of E. coli to 2. 1 A resolution 提交 2010-03-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 P 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.10 Å R-free 0.230
2XAV Ribonucleotide reductase Y731NO2Y and Y730F modified R1 subunit of E. coli 提交 2010-03-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 D 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376 链 E 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.80 Å R-free 0.247
2XAV Ribonucleotide reductase Y731NO2Y and Y730F modified R1 subunit of E. coli 提交 2010-03-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 F 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.80 Å R-free 0.247
2XAV Ribonucleotide reductase Y731NO2Y and Y730F modified R1 subunit of E. coli 提交 2010-03-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 P 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.80 Å R-free 0.247
2XAW Ribonucleotide reductase Y730NO2Y and Y731F modified R1 subunit of E. coli 提交 2010-03-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 F 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 3.10 Å R-free 0.234
2XAW Ribonucleotide reductase Y730NO2Y and Y731F modified R1 subunit of E. coli 提交 2010-03-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 D 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376 链 E 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 3.10 Å R-free 0.234
2XAW Ribonucleotide reductase Y730NO2Y and Y731F modified R1 subunit of E. coli 提交 2010-03-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 P 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 3.10 Å R-free 0.234
2XAX Ribonucleotide reductase Y730NO2Y and Y731A modified R1 subunit of E. coli 提交 2010-04-01	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 P 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.75 Å R-free 0.261
2XAX Ribonucleotide reductase Y730NO2Y and Y731A modified R1 subunit of E. coli 提交 2010-04-01	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 D 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376 链 E 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.75 Å R-free 0.261
2XAX Ribonucleotide reductase Y730NO2Y and Y731A modified R1 subunit of E. coli 提交 2010-04-01	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 F 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.75 Å R-free 0.261
2XAY Ribonucleotide reductase Y730NO2Y and C439A modified R1 subunit of E. coli 提交 2010-04-01	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 D 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376 链 E 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.65 Å R-free 0.235
2XAY Ribonucleotide reductase Y730NO2Y and C439A modified R1 subunit of E. coli 提交 2010-04-01	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 F 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.65 Å R-free 0.235
2XAY Ribonucleotide reductase Y730NO2Y and C439A modified R1 subunit of E. coli 提交 2010-04-01	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 P 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.65 Å R-free 0.235
2XAZ Ribonucleotide reductase Y730NO2Y and C439S modified R1 subunit of E. coli 提交 2010-04-01	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 D 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376 链 E 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.60 Å R-free 0.246
2XAZ Ribonucleotide reductase Y730NO2Y and C439S modified R1 subunit of E. coli 提交 2010-04-01	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 F 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.60 Å R-free 0.246
2XAZ Ribonucleotide reductase Y730NO2Y and C439S modified R1 subunit of E. coli 提交 2010-04-01	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 P 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.60 Å R-free 0.246
2XO4 RIBONUCLEOTIDE REDUCTASE Y730NH2Y MODIFIED R1 SUBUNIT OF E. COLI 提交 2010-08-09	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 F 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.50 Å R-free 0.233
2XO4 RIBONUCLEOTIDE REDUCTASE Y730NH2Y MODIFIED R1 SUBUNIT OF E. COLI 提交 2010-08-09	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 D 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376 链 E 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.50 Å R-free 0.233
2XO4 RIBONUCLEOTIDE REDUCTASE Y730NH2Y MODIFIED R1 SUBUNIT OF E. COLI 提交 2010-08-09	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 P 357–376（20 aa）片段：RIBONUCLEOTIDE REDUCTASE R2-PEPTIDE, RESIDUES 357-376	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.50 Å R-free 0.233
2XO5 RIBONUCLEOTIDE REDUCTASE Y731NH2Y MODIFIED R1 SUBUNIT OF E. COLI 提交 2010-08-09	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 F 357–376（20 aa）片段：R1 BINDING PEPTIDE	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.70 Å R-free 0.228
2XO5 RIBONUCLEOTIDE REDUCTASE Y731NH2Y MODIFIED R1 SUBUNIT OF E. COLI 提交 2010-08-09	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 D 357–376（20 aa）片段：R1 BINDING PEPTIDE 链 E 357–376（20 aa）片段：R1 BINDING PEPTIDE	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.70 Å R-free 0.228
2XO5 RIBONUCLEOTIDE REDUCTASE Y731NH2Y MODIFIED R1 SUBUNIT OF E. COLI 提交 2010-08-09	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 P 357–376（20 aa）片段：R1 BINDING PEPTIDE	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6.	分辨率 2.70 Å R-free 0.228
2XOF Ribonucleotide reductase Y122NO2Y modified R2 subunit of E. coli 提交 2010-08-15	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 2–376（375 aa）链 B 2–376（375 aa）	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	FEO MU-OXO-DIIRON × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION；pH 7.9；VAPOR DIFFUSION USING 90% OF SATURATED NACL, PH 7.9	分辨率 2.20 Å R-free 0.224
3UUS Crystal structure of the dATP inhibited E. coli class Ia ribonucleotide reductase complex 提交 2011-11-28	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 8 PDB 声明：octameric	链 E 2–376（375 aa）链 F 2–376（375 aa）链 G 2–376（375 aa）链 H 2–376（375 aa）	未记录	DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 8 FE FE (III) ION × 8	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；10.8% PEG 3350, 0.18M magnesium acetate, 0.09M MOPS pH 7.5, 0.01M iron (III) chloride, 4.5% glycerol , VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 5.65 Å R-free 0.303
4ERM Crystal structure of the dATP inhibited E. coli class Ia ribonucleotide reductase complex at 4 Angstroms resolution 提交 2012-04-20	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 8 PDB 声明：octameric	链 E 2–376（375 aa）链 F 2–376（375 aa）链 G 2–376（375 aa）链 H 2–376（375 aa）	未记录	DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 8 DAT 2'-DEOXYADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 4 FEO MU-OXO-DIIRON × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Precipitant conditions: 10% PEG 3350, 0.1 M MOPS pH 7.5, 0.2 M Magnesium Acetate, 0.025 M Magnesium Chloride, 0.006 M n-Nonyl-beta-D-maltopyranoside, and 5% glycerol. Mixed 1:1 with protein., VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 3.95 Å R-free 0.284
4ERP Crystal structure of a gemcitabine-diphosphate inhibited E. coli class Ia ribonucleotide reductase complex 提交 2012-04-20	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 8 PDB 声明：octameric	链 F 2–376（375 aa）链 H 2–376（375 aa）	未记录	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 FEO MU-OXO-DIIRON × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Precipitant solution: 25% PEG 3350, 0.1 M HEPES pH 7.5, 0.2 M ammonium acetate, 5% glycerol mixed 2:1 with protein and streak seeded from crystals grown under similar conditions. , VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 4.45 Å R-free 0.267
4ERP Crystal structure of a gemcitabine-diphosphate inhibited E. coli class Ia ribonucleotide reductase complex 提交 2012-04-20	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 8 PDB 声明：octameric	链 E 2–376（375 aa）链 G 2–376（375 aa）	未记录	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 FEO MU-OXO-DIIRON × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Precipitant solution: 25% PEG 3350, 0.1 M HEPES pH 7.5, 0.2 M ammonium acetate, 5% glycerol mixed 2:1 with protein and streak seeded from crystals grown under similar conditions. , VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 4.45 Å R-free 0.267
4R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH SUBSTRATE, GDP AND EFFECTOR DTTP FROM ESCHERICHIA COLI 提交 1997-07-22	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 3 PDB 声明：trimeric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 P 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	TTP THYMIDINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0. THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM GDP WAS ADDED TO THE PROTEIN SOLUTION 48 H BEFORE FREEZING THE PROTEIN CRYSTALS	分辨率 3.20 Å R-free 0.308
4R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH SUBSTRATE, GDP AND EFFECTOR DTTP FROM ESCHERICHIA COLI 提交 1997-07-22	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 10 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	TTP THYMIDINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0. THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM GDP WAS ADDED TO THE PROTEIN SOLUTION 48 H BEFORE FREEZING THE PROTEIN CRYSTALS	分辨率 3.20 Å R-free 0.308
4R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH SUBSTRATE, GDP AND EFFECTOR DTTP FROM ESCHERICHIA COLI 提交 1997-07-22	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 E 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	TTP THYMIDINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0. THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM GDP WAS ADDED TO THE PROTEIN SOLUTION 48 H BEFORE FREEZING THE PROTEIN CRYSTALS	分辨率 3.20 Å R-free 0.308
4R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH SUBSTRATE, GDP AND EFFECTOR DTTP FROM ESCHERICHIA COLI 提交 1997-07-22	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 F 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	TTP THYMIDINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0. THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM GDP WAS ADDED TO THE PROTEIN SOLUTION 48 H BEFORE FREEZING THE PROTEIN CRYSTALS	分辨率 3.20 Å R-free 0.308
4R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH SUBSTRATE, GDP AND EFFECTOR DTTP FROM ESCHERICHIA COLI 提交 1997-07-22	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 4 蛋白异源复合物异源复合物；蛋白 × 15 PDB 声明：pentadecameric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 E 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 P 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	TTP THYMIDINE-5'-TRIPHOSPHATE × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0. THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM GDP WAS ADDED TO THE PROTEIN SOLUTION 48 H BEFORE FREEZING THE PROTEIN CRYSTALS	分辨率 3.20 Å R-free 0.308
4R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH SUBSTRATE, GDP AND EFFECTOR DTTP FROM ESCHERICHIA COLI 提交 1997-07-22	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 5 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 F 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	TTP THYMIDINE-5'-TRIPHOSPHATE × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0. THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM GDP WAS ADDED TO THE PROTEIN SOLUTION 48 H BEFORE FREEZING THE PROTEIN CRYSTALS	分辨率 3.20 Å R-free 0.308
4R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH SUBSTRATE, GDP AND EFFECTOR DTTP FROM ESCHERICHIA COLI 提交 1997-07-22	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 6 蛋白异源复合物异源复合物；蛋白 × 5 PDB 声明：pentameric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 E 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 P 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	TTP THYMIDINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0. THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM GDP WAS ADDED TO THE PROTEIN SOLUTION 48 H BEFORE FREEZING THE PROTEIN CRYSTALS	分辨率 3.20 Å R-free 0.308
4R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH SUBSTRATE, GDP AND EFFECTOR DTTP FROM ESCHERICHIA COLI 提交 1997-07-22	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 7 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 F 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	TTP THYMIDINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0. THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM GDP WAS ADDED TO THE PROTEIN SOLUTION 48 H BEFORE FREEZING THE PROTEIN CRYSTALS	分辨率 3.20 Å R-free 0.308
4R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH SUBSTRATE, GDP AND EFFECTOR DTTP FROM ESCHERICHIA COLI 提交 1997-07-22	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 8 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 E 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	TTP THYMIDINE-5'-TRIPHOSPHATE × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0. THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM GDP WAS ADDED TO THE PROTEIN SOLUTION 48 H BEFORE FREEZING THE PROTEIN CRYSTALS	分辨率 3.20 Å R-free 0.308
4R1R RIBONUCLEOTIDE REDUCTASE R1 PROTEIN WITH SUBSTRATE, GDP AND EFFECTOR DTTP FROM ESCHERICHIA COLI 提交 1997-07-22	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 9 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 E 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	TTP THYMIDINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0. THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20-FOLD EXCESS FO A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION. 10 MM DTTP AND 10 MM GDP WAS ADDED TO THE PROTEIN SOLUTION 48 H BEFORE FREEZING THE PROTEIN CRYSTALS	分辨率 3.20 Å R-free 0.308
5CNS Crystal structure of the dATP inhibited E. coli class Ia ribonucleotide reductase complex bound to CDP and dATP at 2.97 Angstroms resolution 提交 2015-07-18	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 8 PDB 声明：octameric	链 E 2–376（375 aa）链 F 2–376（375 aa）链 G 2–376（375 aa）链 H 2–376（375 aa）	未记录	CDP CYTIDINE-5'-DIPHOSPHATE × 4 DAT 2'-DEOXYADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 8 DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 4 FEO MU-OXO-DIIRON × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；9.5% (w/v) PEG 3350, 100 mM MOPS, 250 mM Mg(CH3COO)2, 25 mM 394 MgCl2, 5% (v/v) glycerol	分辨率 2.98 Å R-free 0.214
5CNT Crystal structure of the dATP inhibited E. coli class Ia ribonucleotide reductase complex bound to UDP and dATP at 3.25 Angstroms resolution 提交 2015-07-18	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 8 PDB 声明：octameric	链 E 2–376（375 aa）链 F 2–376（375 aa）链 G 2–376（375 aa）链 H 2–376（375 aa）	未记录	UDP URIDINE-5'-DIPHOSPHATE × 4 DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 12 MG MAGNESIUM ION × 8 FEO MU-OXO-DIIRON × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；2% (w/v) PEG 3350, 100 mM MOPS pH 7.5, 300 mM Mg(CH3COO)2, 30 mM MgCl2, and 5% (v/v) glycerol	分辨率 3.25 Å R-free 0.221
5CNU Crystal structure of the dATP inhibited E. coli class Ia ribonucleotide reductase complex bound to ADP and dGTP at 3.40 Angstroms resolution 提交 2015-07-18	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 8 PDB 声明：octameric	链 E 2–376（375 aa）链 F 2–376（375 aa）链 G 2–376（375 aa）链 H 2–376（375 aa）	未记录	DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 4 DAT 2'-DEOXYADENOSINE-5'-DIPHOSPHATE × 4 FEO MU-OXO-DIIRON × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；2% (w/v) PEG 3350, 100 mM MOPS pH 7.5, 300 mM Mg(CH3COO)2, 30 mM MgCl2, and 5% (v/v) glycerol	分辨率 3.40 Å R-free 0.221
5CNV Crystal structure of the dATP inhibited E. coli class Ia ribonucleotide reductase complex bound to GDP and TTP at 3.20 Angstroms resolution 提交 2015-07-18	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 8 PDB 声明：octameric	链 E 2–376（375 aa）链 F 2–376（375 aa）链 G 2–376（375 aa）链 H 2–376（375 aa）	未记录	GDP GUANOSINE-5'-DIPHOSPHATE × 4 DAT 2'-DEOXYADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 8 TTP THYMIDINE-5'-TRIPHOSPHATE × 8 FEO MU-OXO-DIIRON × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；2% (w/v) PEG 3350, 100 mM MOPS pH 7.5, 300 mM Mg(CH3COO)2, 30 mM MgCl2, and 5% (v/v) glycerol	分辨率 3.20 Å R-free 0.219
5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 3 PDB 声明：trimeric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 P 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.227
5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 10 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 E 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.227
5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 11 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 F 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.227
5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 12 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.227
5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 E 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.227
5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 F 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.227
5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 4 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 E 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.227
5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 5 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 F 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.227
5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 6 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 E 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.227
5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 7 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.227
5R1R RIBONUCLEOTIDE REDUCTASE E441A MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 8 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 F 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.227
6R1R RIBONUCLEOTIDE REDUCTASE E441D MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 3 PDB 声明：trimeric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 P 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.240
6R1R RIBONUCLEOTIDE REDUCTASE E441D MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 E 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.240
6R1R RIBONUCLEOTIDE REDUCTASE E441D MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 F 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.240
6R1R RIBONUCLEOTIDE REDUCTASE E441D MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 4 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 E 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.240
6R1R RIBONUCLEOTIDE REDUCTASE E441D MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 5 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 F 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.240
6R1R RIBONUCLEOTIDE REDUCTASE E441D MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 7 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 E 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.240
6R1R RIBONUCLEOTIDE REDUCTASE E441D MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 8 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 F 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.240
6R1R RIBONUCLEOTIDE REDUCTASE E441D MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 9 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.240
6W4X Holocomplex of E. coli class Ia ribonucleotide reductase with GDP and TTP 提交 2020-03-11	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 C 1–376（376 aa）链 D 1–376（376 aa）	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	TTP THYMIDINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 FEO MU-OXO-DIIRON × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.6 cryo-EM玻璃化条件冷冻剂 ETHANE；blot for 4.5 seconds before plunging	分辨率 3.60 Å
7AI8 Structure of Ribonucleotide reductase R2 from Escherichia coli collected by still serial crystallography on a COC membrane at a synchrotron source 提交 2020-09-26	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–376（376 aa）	未记录	FE FE (III) ION × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7；294 K；34-37 % PAA 2100, 100 mM HEPES 7.0, 250-450 mM NaCl, 200 mM ammonium sulfate [and] 26% PAA 2100, 100 mM HEPES 7.0, 150 NaCl, 100 Malonate	分辨率 2.10 Å R-free 0.219
7AI9 Structure of Ribonucleotide reductase R2 from Escherichia coli collected by rotation serial crystallography on a COC membrane at a synchrotron source 提交 2020-09-26	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–376（376 aa）	未记录	FE FE (III) ION × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7；294 K；34-37 % PAA 2100, 100 mM HEPES 7.0, 250-450 mM NaCl, 200 mM ammonium sulfate [and] 26% PAA 2100, 100 mM HEPES 7.0, 150 NaCl, 100 Malonate	分辨率 2.00 Å R-free 0.191
7BET Structure of Ribonucleotide reductase R2 from Escherichia coli collected by femtosecond serial crystallography on a COC membrane 提交 2020-12-24	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–376（376 aa）	未记录	FE FE (III) ION × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7；294 K；34-37 % PAA 2100, 100 mM HEPES 7.0, 250-450 mM NaCl, 200 mM ammonium sulfate [and] 26% PAA 2100, 100 mM HEPES 7.0, 150 NaCl, 100 Malonate	分辨率 2.30 Å R-free 0.245
7R1R RIBONUCLEOTIDE REDUCTASE E441Q MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 3 PDB 声明：trimeric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 P 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.231
7R1R RIBONUCLEOTIDE REDUCTASE E441Q MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 E 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.231
7R1R RIBONUCLEOTIDE REDUCTASE E441Q MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 F 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.231
7R1R RIBONUCLEOTIDE REDUCTASE E441Q MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 4 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 F 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.231
7R1R RIBONUCLEOTIDE REDUCTASE E441Q MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 5 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 E 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.231
7R1R RIBONUCLEOTIDE REDUCTASE E441Q MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 7 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 F 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.231
7R1R RIBONUCLEOTIDE REDUCTASE E441Q MUTANT R1 PROTEIN FROM ESCHERICHIA COLI 提交 1997-09-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 8 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 D 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES 链 E 356–375（20 aa）片段：C-TERMINAL PORTION, 20 RESIDUES	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.7 M LITHIUM SULFATE, AND 10 MM MAGNESIUM SULFATE IN 25 MM CITRATE BUFFER AT PH 6.0 THE PROTEIN SOLUTION CONTAINED 17 MG/ML R1 PROTEIN, 20 FOLD EXCESS OF A 20-RESIDUE PEPTIDE CORRESPONDING TO THE C-TERMINUS OF THE R2 SUBUNIT AND IS ESSENTIAL FOR CRYSTALLIZATION	分辨率 3.10 Å R-free 0.231
8VHU Crystal structure of dATP bound E. coli class Ia ribonucleotide reductase alpha construct fused with the C-terminal tail of E. coli class Ia beta subunit 提交 2024-01-02	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 信息不足同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 342–376（35 aa）链 B 342–376（35 aa）	突变：Truncated final 8 residues of alpha,fused C-terminal 35 residues of beta 突变：Truncated final 8 residues of alpha,fused C-terminal 35 residues of beta	DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 3 NA SODIUM ION × 1 CL CHLORIDE ION × 6 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；277.15 K；1.4M NaCl, 8% (w/vol) PEG 6000	分辨率 2.10 Å R-free 0.192
9DB2 Class Ia ribonucleotide reductase with mechanism-based inhibitor N3CDP 提交 2024-08-23	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 C 1–376（376 aa）链 D 1–376（376 aa）	未记录	DTP 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 UNL UNKNOWN LIGAND × 1 A1A3L 4-amino-1-{(3xi)-3-C-amino-2-deoxy-5-O-[(S)-hydroxy(phosphonooxy)phosphoryl]-beta-D-threo-pentofuranosyl}pyrimidin-2(1H)-one × 1 FEO MU-OXO-DIIRON × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.6 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.60 Å
9SIG XFEL structure of oxidised Ribonucleotide reductase R2a Y122F mutant from E. coli 提交 2025-08-28	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 2–376（375 aa）链 B 2–376（375 aa）	突变：Y122F 突变：Y122F	FE FE (III) ION × 4	X-RAY DIFFRACTION X-ray结晶条件 BATCH MODE；pH 5.5；297 K；0.1M Bis-Tris pH 5.5, PEG 3350 25%	分辨率 1.90 Å R-free 0.185
9SIH XFEL structure of Ribonucleotide reductase R2a Y122F mutant from E. coli,reduced form 提交 2025-08-28	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 2–376（375 aa）链 B 2–376（375 aa）	突变：Y122F 突变：Y122F	FE2 FE (II) ION × 4	X-RAY DIFFRACTION X-ray结晶条件 BATCH MODE；pH 5.5；297 K；0.1M Bis-Tris pH 5.5, PEG 3350 25%	分辨率 1.70 Å R-free 0.191
9SJW Serial electron diffraction (SerialED) structure of Y122F mutant Ribonucleotide reductase R2 from E. coli in its oxidised (met) form 提交 2025-09-01	构建体不同突变/修饰不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 2–376（375 aa）链 B 2–376（375 aa）	未记录	FE FE (III) ION × 4	ELECTRON CRYSTALLOGRAPHY cryo-EM缓冲液 pH 5.5；Crystallization was performed using 23.5 uL protein solution, 25 mM HEPES-Na pH 7.0, 50 mM NaCl, and 20 uL crystallization buffer with seeds, 25 percent PEG 3350, 0.1 M Bis-Tris, pH 5.5. cryo-EM玻璃化条件冷冻剂 ETHANE；Excess liquid was manually blotted from the backside for approximately 10 seconds, followed by plunge freezing in liquid ethane.	分辨率 1.80 Å R-free 0.234
9SJX Serial electron diffraction (SerialED) structure of Y122F mutant Ribonucleotide reductase R2 from E. coli in its oxidised (met) form (re-oxidised) 提交 2025-09-01	构建体不同突变/修饰不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 2–376（375 aa）链 B 2–376（375 aa）	未记录	FE FE (III) ION × 4	ELECTRON CRYSTALLOGRAPHY cryo-EM缓冲液 pH 5.5；Crystallization was performed using 23.5 uL protein solution - 25 mM HEPES-Na pH 7.0, 50 mM NaCl and 50 mM sodium dithionite - and 20 uL crystallization buffer containing seeds, 25 percent PEG 3350, 0.1 M Bis-Tris pH 5.5 and 2 mM sodium dithioninte. The crystals were then oxidized through repeated wash with oxygen containing buffer of 12% PEG, 0.05 M Bis-Tris at pH 5.5. cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.00 Å R-free 0.285
9SJY Serial electron diffraction (SerialED) structure of Ribonucleotide reductase R2 from E. coli in its oxidised (met) form 提交 2025-09-01	构建体不同突变/修饰不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 2–376（375 aa）链 B 2–376（375 aa）	未记录	FE FE (III) ION × 4	ELECTRON CRYSTALLOGRAPHY cryo-EM缓冲液 pH 5.5；Crystallization was performed using 23.5 uL protein solution, 25 mM HEPES-Na pH 7.0, 50 mM NaCl, and 20 uL crystallization buffer with seeds, 25 percent PEG 3350, 0.1 M Bis-Tris, pH 5.5. cryo-EM玻璃化条件冷冻剂 ETHANE；Excess liquid was manually blotted from the backside for approximately 10 seconds, followed by plunge freezing in liquid ethane.	分辨率 1.80 Å R-free 0.253
9SJZ Serial electron diffraction (SerialED) structure of Ribonucleotide reductase R2 from E. coli in its oxidised (met) form (re-oxidised) 提交 2025-09-01	构建体不同突变/修饰不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 2–376（375 aa）链 B 2–376（375 aa）	未记录	FE FE (III) ION × 4	ELECTRON CRYSTALLOGRAPHY cryo-EM缓冲液 pH 5.5；Crystallization was performed using 23.5 uL protein solution - 25 mM HEPES-Na pH 7.0, 50 mM NaCl and 50 mM sodium dithionite - and 20 uL crystallization buffer containing seeds, 25 percent PEG 3350, 0.1 M Bis-Tris pH 5.5 and 2 mM sodium dithioninte. The crystals were then oxidized through repeated wash with oxygen containing buffer of 12% PEG, 0.05 M Bis-Tris at pH 5.5. cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 1.70 Å R-free 0.232
9SK1 Serial electron diffraction (SerialED) structure of Y122F mutant Ribonucleotide reductase R2 from E. coli in its reduced (red) form 提交 2025-09-01	构建体不同突变/修饰不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 2–376（375 aa）链 B 2–376（375 aa）	未记录	FE2 FE (II) ION × 4	ELECTRON CRYSTALLOGRAPHY cryo-EM缓冲液 pH 5.5；Crystallization was performed using 23.5 uL protein solution - 25 mM HEPES-Na pH 7.0, 50 mM NaCl and 50 mM sodium dithionite - and 20 uL crystallization buffer containing seeds, 25 percent PEG 3350, 0.1 M Bis-Tris pH 5.5 and 2 mM sodium dithioninte. cryo-EM玻璃化条件冷冻剂 ETHANE；Excess liquid was manually blotted from the backside for approximately 10 seconds, followed by plunge freezing in liquid ethane. Sample was prepared in an inert environment within a glove box.	分辨率 1.80 Å R-free 0.222
9SKG Serial electron diffraction (SerialED) structure of Ribonucleotide reductase R2 from E. coli in its reduced (red) form 提交 2025-09-02	构建体不同突变/修饰不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 2–376（375 aa）链 B 2–376（375 aa）	未记录	FE2 FE (II) ION × 4	ELECTRON CRYSTALLOGRAPHY cryo-EM缓冲液 pH 5.5；Crystallization was performed using 23.5 uL protein solution - 25 mM HEPES-Na pH 7.0, 50 mM NaCl and 50 mM sodium dithionite - and 20 uL crystallization buffer containing seeds, 25 percent PEG 3350, 0.1 M Bis-Tris pH 5.5 and 2 mM sodium dithioninte. cryo-EM玻璃化条件冷冻剂 ETHANE；Excess liquid was manually blotted from the backside for approximately 10 seconds, followed by plunge freezing in liquid ethane. Sample was prepared in an inert environment within a glove box.	分辨率 1.80 Å R-free 0.247
9TCD XFEL structure of oxidised Ribonucleotide reductase R2a Y122F mutant from E. coli, hexagonal P6122 form 提交 2025-11-21	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 2–376（375 aa）	突变：Y122F	FE FE (III) ION × 4	X-RAY DIFFRACTION X-ray结晶条件 BATCH MODE；297 K；4M Sodium Formate	分辨率 2.70 Å R-free 0.254
9TCE XFEL structure of Ribonucleotide reductase R2a Y122F mutant from E. coli,reduced form, hexagonal P6122 提交 2025-11-21	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 2–376（375 aa）	突变：Y122F	FE2 FE (II) ION × 4	X-RAY DIFFRACTION X-ray结晶条件 BATCH MODE；297 K；4M Sodium Formate	分辨率 2.70 Å R-free 0.281

共 62 个其他 PDB 条目、126 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	RIR2_ECOLI
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 1–375； UniProt 1–375 作者链 B； PDB构建体 1–375； UniProt 1–375

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

SAXS 散射曲线 SAXS Profile

暂无 SAXS 图

P(r) 距离分布 P(r) Distribution

暂无 P(r) 图

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	1piu
沉积日期 deposition_date	2003-05-30
结构标题 title	OXIDIZED RIBONUCLEOTIDE REDUCTASE R2-D84E MUTANT CONTAINING OXO-BRIDGED DIFERRIC CLUSTER
关键词 keywords	four-helix bundle, oxo-bridged diferric cluster, OXIDOREDUCTASE; OXIDOREDUCTASE
实验方法 method	X-RAY DIFFRACTION

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3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

该条目暂无 SAXS 数据。

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4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

该条目暂无 P(r) 分析数据。

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5. 晶体学与实验 5. Crystallography & Experiment

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6. 实体与聚合物信息 Entities & Polymers (5)

实体 1 Entity 1 polymer

描述 description	Ribonucleoside-diphosphate reductase 1 beta chain
分子量 formula_weight	43426.9 kDa
EC 编号 ec_number	1.17.4.1
突变 mutation	D84E
来源方法 src_method	man
拷贝数 entity_count	2

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	A,B
原子数 atom_count	5841

AYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQLSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLESIQGRSPNVALLPLISIPELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDELIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFAFAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDLFVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFNTRSNPIPWINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL

来源 Source

来源生物 organism	Escherichia coli
NCBI 分类编号 ncbi_taxonomy_id	562
宿主生物 host_organism	Escherichia coli

实体 2 Entity 2 non-polymer

描述 description	FE (III) ION
分子量 formula_weight	55.8 kDa
来源方法 src_method	syn
拷贝数 entity_count	4

实体 3 Entity 3 non-polymer

描述 description	MERCURY (II) ION
分子量 formula_weight	200.6 kDa
来源方法 src_method	syn
拷贝数 entity_count	10

实体 4 Entity 4 non-polymer

描述 description	OXYGEN ATOM
分子量 formula_weight	16.0 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

实体 5 Entity 5 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	257

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7. 折叠分类 (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

结构域编号 domain_id	d1piua_
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.25 — Ferritin-like
超家族 Superfamily superfamily	a.25.1 — Ferritin-like
家族 Family family	a.25.1.2 — Ribonucleotide reductase-like

结构域编号 domain_id	d1piub_
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.25 — Ferritin-like
超家族 Superfamily superfamily	a.25.1 — Ferritin-like
家族 Family family	a.25.1.2 — Ribonucleotide reductase-like

CATH v4.4 (2 domains)

结构域编号 domain_id	1piuA00
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	620 — Ribonucleotide Reductase, subunit A
同源超家族 H-superfamily homologous superfamily	20 — Ribonucleotide Reductase, subunit A

结构域编号 domain_id	1piuB00
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	620 — Ribonucleotide Reductase, subunit A
同源超家族 H-superfamily homologous superfamily	20 — Ribonucleotide Reductase, subunit A

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8. 引用文献 (1)

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晶胞边长 a length_a	74.100 Å
晶胞边长 b length_b	84.600 Å
晶胞边长 c length_c	114.800 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	8

空间群 space_group	P 21 21 21
空间群编号 int_tables_number	19
晶系 crystal_system	—

分辨率 resolution	2.200 Å
最低分辨率 resolution_low	20.750 Å
R 因子 r_factor	0.1910
R_work r_work	0.1910
R_free r_free	0.2320