1r1r

RIBONUCLEOTIDE REDUCTASE R1 PROTEIN MUTANT Y730F WITH A REDUCED ACTIVE SITE FROM ESCHERICHIA COLI

Method: X-RAY DIFFRACTION Dmax: 168.6 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 1r1r

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1r1r
下载 Download

1. 结构基本信息 1. Structure Basics

条目编号 entry_id1r1r
沉积日期 deposition_date1997-07-15
结构标题 titleRIBONUCLEOTIDE REDUCTASE R1 PROTEIN MUTANT Y730F WITH A REDUCED ACTIVE SITE FROM ESCHERICHIA COLI
关键词 keywordsOXIDOREDUCTASE, DEOXYRIBONUCLEOTIDE SYNTHESIS, RADICAL CHEMISTRY, REDOX CENTER, RIBONUCLEOTIDE REDUCTASE; OXIDOREDUCTASE
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier50.49
回转半径 Rg (电子) rg_electron50.24
零角强度 I(0) i0925686000.00
分子量 molecular_weight254570.0 kDa
排除体积 excluded_volume318800 ų
包络体积 envelope_volume446820 ų
水化壳体积 shell_volume72917 ų
包络直径 envelope_diameter159.5
壳层 Rg shell_rg55.34
包络 Rg envelope_rg48.93
形状 Rg shape_rg50.26
总 Rg total_rg50.32
总原子数 total_atoms17946
残基数 n_residues2253
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax168.6
Rg (实空间) rg_real50.45
Rg 误差 (实空间) rg_real_error1.66
I(0) (实空间) i0_real9.2570e+08
I(0) 误差 (实空间) i0_real_error1.9530e+07
Rg (倒空间) rg_reciprocal50.51
I(0) (倒空间) i0_reciprocal925700000.0000
解质量估计 total_estimate0.8838
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary76.1
偏度 Skewness skewness0.152
峰度 Kurtosis kurtosis-0.752
角度范围 angular_range— – 0.1550 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha107200000.0000
实空间数据点数 n_real_points32
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.874; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.955; Smooth: 0.908

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (3)

6. 折叠分类 (SCOP + CATH) 9 domains

SCOP 2.08 (6 domains)

结构域编号 domain_idd1r1ra1
类 Class classa — All alpha proteins
折叠类型 Fold folda.98 — R1 subunit of ribonucleotide reductase, N-terminal domain
超家族 Superfamily superfamilya.98.1 — R1 subunit of ribonucleotide reductase, N-terminal domain
家族 Family familya.98.1.1 — R1 subunit of ribonucleotide reductase, N-terminal domain
结构域编号 domain_idd1r1ra2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.7 — PFL-like glycyl radical enzymes
超家族 Superfamily superfamilyc.7.1 — PFL-like glycyl radical enzymes
家族 Family familyc.7.1.2 — R1 subunit of ribonucleotide reductase, C-terminal domain
结构域编号 domain_idd1r1rb1
类 Class classa — All alpha proteins
折叠类型 Fold folda.98 — R1 subunit of ribonucleotide reductase, N-terminal domain
超家族 Superfamily superfamilya.98.1 — R1 subunit of ribonucleotide reductase, N-terminal domain
家族 Family familya.98.1.1 — R1 subunit of ribonucleotide reductase, N-terminal domain
结构域编号 domain_idd1r1rb2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.7 — PFL-like glycyl radical enzymes
超家族 Superfamily superfamilyc.7.1 — PFL-like glycyl radical enzymes
家族 Family familyc.7.1.2 — R1 subunit of ribonucleotide reductase, C-terminal domain
结构域编号 domain_idd1r1rc1
类 Class classa — All alpha proteins
折叠类型 Fold folda.98 — R1 subunit of ribonucleotide reductase, N-terminal domain
超家族 Superfamily superfamilya.98.1 — R1 subunit of ribonucleotide reductase, N-terminal domain
家族 Family familya.98.1.1 — R1 subunit of ribonucleotide reductase, N-terminal domain
结构域编号 domain_idd1r1rc2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.7 — PFL-like glycyl radical enzymes
超家族 Superfamily superfamilyc.7.1 — PFL-like glycyl radical enzymes
家族 Family familyc.7.1.2 — R1 subunit of ribonucleotide reductase, C-terminal domain

CATH v4.4 (3 domains)

结构域编号 domain_id1r1rA02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology70 — Anaerobic Ribonucleotide-triphosphate Reductase Large Chain
同源超家族 H-superfamily homologous superfamily20
结构域编号 domain_id1r1rB02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology70 — Anaerobic Ribonucleotide-triphosphate Reductase Large Chain
同源超家族 H-superfamily homologous superfamily20
结构域编号 domain_id1r1rC02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology70 — Anaerobic Ribonucleotide-triphosphate Reductase Large Chain
同源超家族 H-superfamily homologous superfamily20

7. 引用文献 (3)

8. 文件与曲线 (10)