1q0k

Crystal structure of Ni-containing superoxide dismutase with Ni-ligation corresponding to the thiosulfate-reduced state

Method: X-RAY DIFFRACTION Dmax: 145.6 Å Quality: REASONABLE

SAXS 散射曲线 SAXS Profile

SAXS profile for 1q0k

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1q0k
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1q0k
沉积日期 deposition_date2003-07-16
结构标题 titleCrystal structure of Ni-containing superoxide dismutase with Ni-ligation corresponding to the thiosulfate-reduced state
关键词 keywordsHOMOHEXAMER OF FOUR-HELIX BUNDLES, OXIDOREDUCTASE; OXIDOREDUCTASE
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier41.75
回转半径 Rg (电子) rg_electron41.96
零角强度 I(0) i0401406000.00
分子量 molecular_weight161410.0 kDa
排除体积 excluded_volume200300 ų
包络体积 envelope_volume254410 ų
水化壳体积 shell_volume50911 ų
包络直径 envelope_diameter140.3
壳层 Rg shell_rg46.50
包络 Rg envelope_rg41.40
形状 Rg shape_rg42.00
总 Rg total_rg42.03
总原子数 total_atoms11304
残基数 n_residues1404
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax145.6
Rg (实空间) rg_real41.97
Rg 误差 (实空间) rg_real_error1.67
I(0) (实空间) i0_real4.0140e+08
I(0) 误差 (实空间) i0_real_error7.4030e+06
Rg (倒空间) rg_reciprocal41.76
I(0) (倒空间) i0_reciprocal401300000.0000
解质量估计 total_estimate0.6968
解质量评级 solution_quality REASONABLE a REASONABLE solution
P(r) 峰数 n_peaks4
主峰位置 r_peak_primary34.5
偏度 Skewness skewness0.387
峰度 Kurtosis kurtosis-0.773
角度范围 angular_range— – 0.1900 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha430100000.0000
实空间数据点数 n_real_points39
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.427; Stabil: 0.977; Sysdev: 1.000; Positv: 1.000; Valcen: 0.843; Smooth: 0.000

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (5)

6. 折叠分类 (SCOP + CATH) 24 domains

SCOP 2.08 (12 domains)

结构域编号 domain_idd1q0ka_
类 Class classa — All alpha proteins
折叠类型 Fold folda.24 — Four-helical up-and-down bundle
超家族 Superfamily superfamilya.24.22 — Nickel-containing superoxide dismutase, NiSOD
家族 Family familya.24.22.1 — Nickel-containing superoxide dismutase, NiSOD
结构域编号 domain_idd1q0kb_
类 Class classa — All alpha proteins
折叠类型 Fold folda.24 — Four-helical up-and-down bundle
超家族 Superfamily superfamilya.24.22 — Nickel-containing superoxide dismutase, NiSOD
家族 Family familya.24.22.1 — Nickel-containing superoxide dismutase, NiSOD
结构域编号 domain_idd1q0kc_
类 Class classa — All alpha proteins
折叠类型 Fold folda.24 — Four-helical up-and-down bundle
超家族 Superfamily superfamilya.24.22 — Nickel-containing superoxide dismutase, NiSOD
家族 Family familya.24.22.1 — Nickel-containing superoxide dismutase, NiSOD
结构域编号 domain_idd1q0kd_
类 Class classa — All alpha proteins
折叠类型 Fold folda.24 — Four-helical up-and-down bundle
超家族 Superfamily superfamilya.24.22 — Nickel-containing superoxide dismutase, NiSOD
家族 Family familya.24.22.1 — Nickel-containing superoxide dismutase, NiSOD
结构域编号 domain_idd1q0ke_
类 Class classa — All alpha proteins
折叠类型 Fold folda.24 — Four-helical up-and-down bundle
超家族 Superfamily superfamilya.24.22 — Nickel-containing superoxide dismutase, NiSOD
家族 Family familya.24.22.1 — Nickel-containing superoxide dismutase, NiSOD
结构域编号 domain_idd1q0kf_
类 Class classa — All alpha proteins
折叠类型 Fold folda.24 — Four-helical up-and-down bundle
超家族 Superfamily superfamilya.24.22 — Nickel-containing superoxide dismutase, NiSOD
家族 Family familya.24.22.1 — Nickel-containing superoxide dismutase, NiSOD
结构域编号 domain_idd1q0kg_
类 Class classa — All alpha proteins
折叠类型 Fold folda.24 — Four-helical up-and-down bundle
超家族 Superfamily superfamilya.24.22 — Nickel-containing superoxide dismutase, NiSOD
家族 Family familya.24.22.1 — Nickel-containing superoxide dismutase, NiSOD
结构域编号 domain_idd1q0kh_
类 Class classa — All alpha proteins
折叠类型 Fold folda.24 — Four-helical up-and-down bundle
超家族 Superfamily superfamilya.24.22 — Nickel-containing superoxide dismutase, NiSOD
家族 Family familya.24.22.1 — Nickel-containing superoxide dismutase, NiSOD
结构域编号 domain_idd1q0ki_
类 Class classa — All alpha proteins
折叠类型 Fold folda.24 — Four-helical up-and-down bundle
超家族 Superfamily superfamilya.24.22 — Nickel-containing superoxide dismutase, NiSOD
家族 Family familya.24.22.1 — Nickel-containing superoxide dismutase, NiSOD
结构域编号 domain_idd1q0kj_
类 Class classa — All alpha proteins
折叠类型 Fold folda.24 — Four-helical up-and-down bundle
超家族 Superfamily superfamilya.24.22 — Nickel-containing superoxide dismutase, NiSOD
家族 Family familya.24.22.1 — Nickel-containing superoxide dismutase, NiSOD
结构域编号 domain_idd1q0kk_
类 Class classa — All alpha proteins
折叠类型 Fold folda.24 — Four-helical up-and-down bundle
超家族 Superfamily superfamilya.24.22 — Nickel-containing superoxide dismutase, NiSOD
家族 Family familya.24.22.1 — Nickel-containing superoxide dismutase, NiSOD
结构域编号 domain_idd1q0kl_
类 Class classa — All alpha proteins
折叠类型 Fold folda.24 — Four-helical up-and-down bundle
超家族 Superfamily superfamilya.24.22 — Nickel-containing superoxide dismutase, NiSOD
家族 Family familya.24.22.1 — Nickel-containing superoxide dismutase, NiSOD

CATH v4.4 (12 domains)

结构域编号 domain_id1q0kA00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology120 — Four Helix Bundle (Hemerythrin (Met), subunit A)
同源超家族 H-superfamily homologous superfamily400 — Nickel-containing superoxide dismutase
结构域编号 domain_id1q0kB00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology120 — Four Helix Bundle (Hemerythrin (Met), subunit A)
同源超家族 H-superfamily homologous superfamily400 — Nickel-containing superoxide dismutase
结构域编号 domain_id1q0kC00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology120 — Four Helix Bundle (Hemerythrin (Met), subunit A)
同源超家族 H-superfamily homologous superfamily400 — Nickel-containing superoxide dismutase
结构域编号 domain_id1q0kD00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology120 — Four Helix Bundle (Hemerythrin (Met), subunit A)
同源超家族 H-superfamily homologous superfamily400 — Nickel-containing superoxide dismutase
结构域编号 domain_id1q0kE00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology120 — Four Helix Bundle (Hemerythrin (Met), subunit A)
同源超家族 H-superfamily homologous superfamily400 — Nickel-containing superoxide dismutase
结构域编号 domain_id1q0kF00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology120 — Four Helix Bundle (Hemerythrin (Met), subunit A)
同源超家族 H-superfamily homologous superfamily400 — Nickel-containing superoxide dismutase
结构域编号 domain_id1q0kG00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology120 — Four Helix Bundle (Hemerythrin (Met), subunit A)
同源超家族 H-superfamily homologous superfamily400 — Nickel-containing superoxide dismutase
结构域编号 domain_id1q0kH00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology120 — Four Helix Bundle (Hemerythrin (Met), subunit A)
同源超家族 H-superfamily homologous superfamily400 — Nickel-containing superoxide dismutase
结构域编号 domain_id1q0kI00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology120 — Four Helix Bundle (Hemerythrin (Met), subunit A)
同源超家族 H-superfamily homologous superfamily400 — Nickel-containing superoxide dismutase
结构域编号 domain_id1q0kJ00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology120 — Four Helix Bundle (Hemerythrin (Met), subunit A)
同源超家族 H-superfamily homologous superfamily400 — Nickel-containing superoxide dismutase
结构域编号 domain_id1q0kK00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology120 — Four Helix Bundle (Hemerythrin (Met), subunit A)
同源超家族 H-superfamily homologous superfamily400 — Nickel-containing superoxide dismutase
结构域编号 domain_id1q0kL00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology120 — Four Helix Bundle (Hemerythrin (Met), subunit A)
同源超家族 H-superfamily homologous superfamily400 — Nickel-containing superoxide dismutase

7. 引用文献 (1)

8. 文件与曲线 (10)