1rax

RA-DOMAIN OF RAL GUANOSINE-NUCLEOTIDE DISSOCIATION STIMULATOR

Method: SOLUTION NMR D_max: 53.4 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	1rax
沉积日期 deposition_date	1999-03-13
结构标题 title	RA-DOMAIN OF RAL GUANOSINE-NUCLEOTIDE DISSOCIATION STIMULATOR
关键词 keywords	RAS-BINDING DOMAIN, RALGEF, RALGDS, RAS, RA; RAS-BINDING DOMAIN
实验方法 method	SOLUTION NMR

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	13.74 Å
回转半径 Rg (电子) rg_electron	13.07 Å
零角强度 I(0) i0	169770000.00
分子量 molecular_weight	106960.0 kDa
排除体积 excluded_volume	133160 Å³
包络体积 envelope_volume	24588 Å³
水化壳体积 shell_volume	13682 Å³
包络直径 envelope_diameter	54.8 Å
壳层 Rg shell_rg	21.27 Å
包络 Rg envelope_rg	16.02 Å
形状 Rg shape_rg	13.06 Å
总 Rg total_rg	13.43 Å
总原子数 total_atoms	14900
残基数 n_residues	930
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	53.4 Å
Rg (实空间) rg_real	13.69 Å
Rg 误差 (实空间) rg_real_error	0.47 Å
I(0) (实空间) i0_real	1.6980e+08
I(0) 误差 (实空间) i0_real_error	2.1870e+06
Rg (倒空间) rg_reciprocal	13.69 Å
I(0) (倒空间) i0_reciprocal	169800000.0000
解质量估计 total_estimate	0.7937
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	16.9 Å
偏度 Skewness skewness	0.271
峰度 Kurtosis kurtosis	-0.013
角度范围 angular_range	— – 0.5000 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	260800.0000
实空间数据点数 n_real_points	80
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.481; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.872; Smooth: 0.999

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (1)

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6. 折叠分类 (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

结构域编号 domain_id	d1raxa_
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.15 — beta-Grasp (ubiquitin-like)
超家族 Superfamily superfamily	d.15.1 — Ubiquitin-like
家族 Family family	d.15.1.5 — Ras-binding domain, RBD

CATH v4.4 (1 domains)

结构域编号 domain_id	1raxA00
类 Class class	3 — Alpha Beta
架构 Architecture architecture	10 — Roll
拓扑 Topology topology	20 — Ubiquitin-like (UB roll)
同源超家族 H-superfamily homologous superfamily	90 — Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

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7. 引用文献 (7)

High-Resolution Structure of the Ra-Domain of Human Ralgds and a Dynamics Study of its Binding Loop to Ras

To be Published

PRIMARY

Three-dimensional structure of the Ras-interacting domain of RalGDS.

Nat.Struct.Biol. (1997)

PMID: 9253406 · DOI: 10.1038/nsb0897-609

Structure of the Ras-binding domain of RalGEF and implications for Ras binding and signalling.

Nat.Struct.Biol. (1997)

PMID: 9302994 · DOI: 10.1038/nsb0997-694

;Differential interaction of the ras family GTP-binding proteins H-Ras, Rap1A, and R-Ras with the putative effector molecules Raf kinase and Ral-guanine nucleotide exchange factor. ;

J.Biol.Chem. (1996)

PMID: 8636102 · DOI: 10.1074/jbc.271.12.6794

;Identification of the guanine nucleotide dissociation stimulator for Ral as a putative effector molecule of R-ras, H-ras, K-ras, and Rap. ;

Proc.Natl.Acad.Sci.USA (1994)

PMID: 7809086 · DOI: 10.1073/pnas.91.26.12609

Activated Ras interacts with the Ral guanine nucleotide dissociation stimulator.

Proc.Natl.Acad.Sci.USA (1994)

PMID: 7972015 · DOI: 10.1073/pnas.91.23.11089

Characterization of a guanine nucleotide dissociation stimulator for a ras-related GTPase.

Embo J. (1993)

PMID: 8094051 · DOI: 10.1002/j.1460-2075.1993.tb05662.x

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	1

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

描述 description	PROTEIN (RA-DOMAIN OF RAL GUANOSINE DISSOCIATION STIMULATOR)
分子量 formula_weight	12888.6 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体类型 type	polypeptide(L)
链编号 strand_id	A
原子数 atom_count	14900

来源生物 organism	Homo sapiens
通用名 common_name	human
NCBI 分类编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Escherichia coli

RA-DOMAIN OF RAL GUANOSINE-NUCLEOTIDE DISSOCIATION STIMULATOR

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

5. 实体与聚合体信息 (1)

Entity 1 polymer

聚合体 Polymer

来源 Source

6. 折叠分类 (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

CATH v4.4 (1 domains)

7. 引用文献 (7)

8. 文件与曲线 (10)

Files (7)

Curves (3)

fit	/app/data/saxs_computation/ra/1rax/1rax.fit
pddf	/app/data/pr_computation/ra/1rax/1rax.pr.out
profile	/app/data/saxs_computation/ra/1rax/1rax.dat