1rh7

Crystal Structure of Resistin-like beta

Method: X-RAY DIFFRACTION D_max: 138.1 Å Quality: REASONABLE

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	1rh7
沉积日期 deposition_date	2003-11-13
结构标题 title	Crystal Structure of Resistin-like beta
关键词 keywords	;HORMONE; GLUCOSE UPTAKE; RESISTIN/FIZZ FAMILY, Structural Genomics, PSI, Protein Structure Initiative, New York SGX Research Center for Structural Genomics, NYSGXRC, HORMONE-GROWTH FACTOR COMPLEX ;; HORMONE/GROWTH FACTOR
实验方法 method	X-RAY DIFFRACTION

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	47.64 Å
回转半径 Rg (电子) rg_electron	49.13 Å
零角强度 I(0) i0	53410700.00
分子量 molecular_weight	49874.0 kDa
排除体积 excluded_volume	58195 Å³
包络体积 envelope_volume	86054 Å³
水化壳体积 shell_volume	19078 Å³
包络直径 envelope_diameter	147.7 Å
壳层 Rg shell_rg	37.48 Å
包络 Rg envelope_rg	47.15 Å
形状 Rg shape_rg	49.19 Å
总 Rg total_rg	48.44 Å
总原子数 total_atoms	3335
残基数 n_residues	486
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	138.1 Å
Rg (实空间) rg_real	48.81 Å
Rg 误差 (实空间) rg_real_error	1.84 Å
I(0) (实空间) i0_real	5.3410e+07
I(0) 误差 (实空间) i0_real_error	1.0250e+06
Rg (倒空间) rg_reciprocal	47.65 Å
I(0) (倒空间) i0_reciprocal	53330000.0000
解质量估计 total_estimate	0.5398
解质量评级 solution_quality	REASONABLE a REASONABLE solution
P(r) 峰数 n_peaks	3
主峰位置 r_peak_primary	20.9 Å
偏度 Skewness skewness	0.361
峰度 Kurtosis kurtosis	-1.234
角度范围 angular_range	— – 0.1650 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	593700.0000
实空间数据点数 n_real_points	34
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.005; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.000; Smooth: 0.000

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (4)

Entity 1 polymer

描述 description	Resistin-like beta
分子量 formula_weight	8642.9 kDa
来源方法 src_method	man
拷贝数 entity_count	6

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D,E,F
原子数 atom_count	3475

CSFESLVDQRIKEALSRQEPKTISCTSVTSSGRLASCPAGMVVTGCACGYGCGSWDIRNGNTCHCQCSVMDWASARCCRMA

来源 Source

来源生物 organism	Mus musculus
通用名 common_name	house mouse
NCBI 分类编号 ncbi_taxonomy_id	10090
宿主生物 host_organism	Homo sapiens

Entity 2 non-polymer

描述 description	PLATINUM (II) ION
分子量 formula_weight	195.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	6

Entity 3 non-polymer

描述 description	HEXAETHYLENE GLYCOL
分子量 formula_weight	282.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

Entity 4 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	140

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6. 折叠分类 (SCOP + CATH) 18 domains

SCOP 2.08 (6 domains)

结构域编号 domain_id	d1rh7a_
类 Class class	g — Small proteins
折叠类型 Fold fold	g.77 — Resistin
超家族 Superfamily superfamily	g.77.1 — Resistin
家族 Family family	g.77.1.1 — Resistin

结构域编号 domain_id	d1rh7b_
类 Class class	g — Small proteins
折叠类型 Fold fold	g.77 — Resistin
超家族 Superfamily superfamily	g.77.1 — Resistin
家族 Family family	g.77.1.1 — Resistin

结构域编号 domain_id	d1rh7c_
类 Class class	g — Small proteins
折叠类型 Fold fold	g.77 — Resistin
超家族 Superfamily superfamily	g.77.1 — Resistin
家族 Family family	g.77.1.1 — Resistin

结构域编号 domain_id	d1rh7d_
类 Class class	g — Small proteins
折叠类型 Fold fold	g.77 — Resistin
超家族 Superfamily superfamily	g.77.1 — Resistin
家族 Family family	g.77.1.1 — Resistin

结构域编号 domain_id	d1rh7e_
类 Class class	g — Small proteins
折叠类型 Fold fold	g.77 — Resistin
超家族 Superfamily superfamily	g.77.1 — Resistin
家族 Family family	g.77.1.1 — Resistin

结构域编号 domain_id	d1rh7f_
类 Class class	g — Small proteins
折叠类型 Fold fold	g.77 — Resistin
超家族 Superfamily superfamily	g.77.1 — Resistin
家族 Family family	g.77.1.1 — Resistin

CATH v4.4 (12 domains)

结构域编号 domain_id	1rh7A01
类 Class class	6 — Special
架构 Architecture architecture	10 — Helix non-globular
拓扑 Topology topology	250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily	3290 —

结构域编号 domain_id	1rh7A02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	60 — Sandwich
拓扑 Topology topology	40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily	4230 — Resistin head domain

结构域编号 domain_id	1rh7B01
类 Class class	6 — Special
架构 Architecture architecture	10 — Helix non-globular
拓扑 Topology topology	250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily	3290 —

结构域编号 domain_id	1rh7B02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	60 — Sandwich
拓扑 Topology topology	40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily	4230 — Resistin head domain

结构域编号 domain_id	1rh7C01
类 Class class	6 — Special
架构 Architecture architecture	10 — Helix non-globular
拓扑 Topology topology	250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily	3290 —

结构域编号 domain_id	1rh7C02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	60 — Sandwich
拓扑 Topology topology	40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily	4230 — Resistin head domain

结构域编号 domain_id	1rh7D01
类 Class class	6 — Special
架构 Architecture architecture	10 — Helix non-globular
拓扑 Topology topology	250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily	3290 —

结构域编号 domain_id	1rh7D02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	60 — Sandwich
拓扑 Topology topology	40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily	4230 — Resistin head domain

结构域编号 domain_id	1rh7E01
类 Class class	6 — Special
架构 Architecture architecture	10 — Helix non-globular
拓扑 Topology topology	250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily	3290 —

结构域编号 domain_id	1rh7E02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	60 — Sandwich
拓扑 Topology topology	40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily	4230 — Resistin head domain

结构域编号 domain_id	1rh7F01
类 Class class	6 — Special
架构 Architecture architecture	10 — Helix non-globular
拓扑 Topology topology	250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily	3290 —

结构域编号 domain_id	1rh7F02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	60 — Sandwich
拓扑 Topology topology	40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily	4230 — Resistin head domain

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7. 引用文献 (1)

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晶胞边长 a length_a	57.604 Å
晶胞边长 b length_b	86.016 Å
晶胞边长 c length_c	283.406 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	48

空间群 space_group	I 2 2 2
空间群编号 int_tables_number	23
晶系 crystal_system	—

分辨率 resolution	3.106 Å
最低分辨率 resolution_low	14.960 Å
R 因子 r_factor	0.2043
R_work r_work	0.2012
R_free r_free	0.2638

fit	/app/data/saxs_computation/rh/1rh7/1rh7.fit
pddf	/app/data/pr_computation/rh/1rh7/1rh7.pr.out
profile	/app/data/saxs_computation/rh/1rh7/1rh7.dat