1rqf

Structure of CK2 beta subunit crystallized in the presence of a p21WAF1 peptide

Method: X-RAY DIFFRACTION

SAXS 散射曲线 SAXS Profile

暂无 SAXS 图

P(r) 距离分布 P(r) Distribution

暂无 P(r) 图

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	1rqf
沉积日期 deposition_date	2003-12-05
结构标题 title	Structure of CK2 beta subunit crystallized in the presence of a p21WAF1 peptide
关键词 keywords	CASEIN KINASE BETA SUBUNIT, SER/THR PROTEIN KINASE, CYCLIN-DEPENDENT KINASE INHIBITOR, ZN FINGER, TRANSFERASE; TRANSFERASE
实验方法 method	X-RAY DIFFRACTION

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

该条目暂无 SAXS 数据。

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

该条目暂无 P(r) 分析数据。

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (7)

Entity 1 polymer

描述 description	Casein kinase II beta chain
分子量 formula_weight	21260.0 kDa
EC 编号 ec_number	2.7.1.37
来源方法 src_method	man
拷贝数 entity_count	8

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B,D,E,G,H,J,K
原子数 atom_count	11216

GSPGIQMSSSEEVSWISWFCGLRGNEFFCEVDEDYIQDKFNLTGLNEQVPHYRQALDMILDLEPDEELEDNPNQSDLIEQAAEMLYGLIHARYILTNRGIAQMLEKYQQGDFGYCPRVYCENQPMLPIGLSDIPGEAMVKLYCPKCMDVYTPKSSRHHHTDGAYFGTGFPHMLFMVHPEYRPKR

来源 Source

来源生物 organism	Xenopus laevis
通用名 common_name	African clawed frog
NCBI 分类编号 ncbi_taxonomy_id	8355
宿主生物 host_organism	Escherichia coli BL21(DE3)

Entity 2 polymer

描述 description	Disordered segment of Cyclin-dependent kinase inhibitor 1
分子量 formula_weight	358.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	M,N,T

XXXX

Entity 3 polymer

描述 description	Disordered segment of Cyclin-dependent kinase inhibitor 1
分子量 formula_weight	698.9 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	O

XXXXXXXX

Entity 4 polymer

描述 description	Disordered segment of Cyclin-dependent kinase inhibitor 1
分子量 formula_weight	273.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	P,S

XXX

Entity 5 non-polymer

描述 description	ZINC ION
分子量 formula_weight	65.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	8

Entity 6 non-polymer

描述 description	UNKNOWN LIGAND
分子量 formula_weight	103.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

Entity 7 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	65

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6. 折叠分类 (SCOP + CATH) 24 domains

SCOP 2.08 (8 domains)

结构域编号 domain_id	d1rqfa_
类 Class class	g — Small proteins
折叠类型 Fold fold	g.41 — Rubredoxin-like
超家族 Superfamily superfamily	g.41.4 — Casein kinase II beta subunit
家族 Family family	g.41.4.1 — Casein kinase II beta subunit

结构域编号 domain_id	d1rqfb_
类 Class class	g — Small proteins
折叠类型 Fold fold	g.41 — Rubredoxin-like
超家族 Superfamily superfamily	g.41.4 — Casein kinase II beta subunit
家族 Family family	g.41.4.1 — Casein kinase II beta subunit

结构域编号 domain_id	d1rqfd_
类 Class class	g — Small proteins
折叠类型 Fold fold	g.41 — Rubredoxin-like
超家族 Superfamily superfamily	g.41.4 — Casein kinase II beta subunit
家族 Family family	g.41.4.1 — Casein kinase II beta subunit

结构域编号 domain_id	d1rqfe_
类 Class class	g — Small proteins
折叠类型 Fold fold	g.41 — Rubredoxin-like
超家族 Superfamily superfamily	g.41.4 — Casein kinase II beta subunit
家族 Family family	g.41.4.1 — Casein kinase II beta subunit

结构域编号 domain_id	d1rqfg_
类 Class class	g — Small proteins
折叠类型 Fold fold	g.41 — Rubredoxin-like
超家族 Superfamily superfamily	g.41.4 — Casein kinase II beta subunit
家族 Family family	g.41.4.1 — Casein kinase II beta subunit

结构域编号 domain_id	d1rqfh_
类 Class class	g — Small proteins
折叠类型 Fold fold	g.41 — Rubredoxin-like
超家族 Superfamily superfamily	g.41.4 — Casein kinase II beta subunit
家族 Family family	g.41.4.1 — Casein kinase II beta subunit

结构域编号 domain_id	d1rqfj_
类 Class class	g — Small proteins
折叠类型 Fold fold	g.41 — Rubredoxin-like
超家族 Superfamily superfamily	g.41.4 — Casein kinase II beta subunit
家族 Family family	g.41.4.1 — Casein kinase II beta subunit

结构域编号 domain_id	d1rqfk_
类 Class class	g — Small proteins
折叠类型 Fold fold	g.41 — Rubredoxin-like
超家族 Superfamily superfamily	g.41.4 — Casein kinase II beta subunit
家族 Family family	g.41.4.1 — Casein kinase II beta subunit

CATH v4.4 (16 domains)

结构域编号 domain_id	1rqfA01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	1820 — protein kinase ck2 holoenzyme, chain C, domain 1
同源超家族 H-superfamily homologous superfamily	10 — protein kinase ck2 holoenzyme, chain C, domain 1

结构域编号 domain_id	1rqfA02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	20 — Single Sheet
拓扑 Topology topology	25 — N-terminal domain of TfIIb
同源超家族 H-superfamily homologous superfamily	20 —

结构域编号 domain_id	1rqfB01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	1820 — protein kinase ck2 holoenzyme, chain C, domain 1
同源超家族 H-superfamily homologous superfamily	10 — protein kinase ck2 holoenzyme, chain C, domain 1

结构域编号 domain_id	1rqfB02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	20 — Single Sheet
拓扑 Topology topology	25 — N-terminal domain of TfIIb
同源超家族 H-superfamily homologous superfamily	20 —

结构域编号 domain_id	1rqfD01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	1820 — protein kinase ck2 holoenzyme, chain C, domain 1
同源超家族 H-superfamily homologous superfamily	10 — protein kinase ck2 holoenzyme, chain C, domain 1

结构域编号 domain_id	1rqfD02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	20 — Single Sheet
拓扑 Topology topology	25 — N-terminal domain of TfIIb
同源超家族 H-superfamily homologous superfamily	20 —

结构域编号 domain_id	1rqfE01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	1820 — protein kinase ck2 holoenzyme, chain C, domain 1
同源超家族 H-superfamily homologous superfamily	10 — protein kinase ck2 holoenzyme, chain C, domain 1

结构域编号 domain_id	1rqfE02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	20 — Single Sheet
拓扑 Topology topology	25 — N-terminal domain of TfIIb
同源超家族 H-superfamily homologous superfamily	20 —

结构域编号 domain_id	1rqfG01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	1820 — protein kinase ck2 holoenzyme, chain C, domain 1
同源超家族 H-superfamily homologous superfamily	10 — protein kinase ck2 holoenzyme, chain C, domain 1

结构域编号 domain_id	1rqfG02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	20 — Single Sheet
拓扑 Topology topology	25 — N-terminal domain of TfIIb
同源超家族 H-superfamily homologous superfamily	20 —

结构域编号 domain_id	1rqfH01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	1820 — protein kinase ck2 holoenzyme, chain C, domain 1
同源超家族 H-superfamily homologous superfamily	10 — protein kinase ck2 holoenzyme, chain C, domain 1

结构域编号 domain_id	1rqfH02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	20 — Single Sheet
拓扑 Topology topology	25 — N-terminal domain of TfIIb
同源超家族 H-superfamily homologous superfamily	20 —

结构域编号 domain_id	1rqfJ01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	1820 — protein kinase ck2 holoenzyme, chain C, domain 1
同源超家族 H-superfamily homologous superfamily	10 — protein kinase ck2 holoenzyme, chain C, domain 1

结构域编号 domain_id	1rqfJ02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	20 — Single Sheet
拓扑 Topology topology	25 — N-terminal domain of TfIIb
同源超家族 H-superfamily homologous superfamily	20 —

结构域编号 domain_id	1rqfK01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	1820 — protein kinase ck2 holoenzyme, chain C, domain 1
同源超家族 H-superfamily homologous superfamily	10 — protein kinase ck2 holoenzyme, chain C, domain 1

结构域编号 domain_id	1rqfK02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	20 — Single Sheet
拓扑 Topology topology	25 — N-terminal domain of TfIIb
同源超家族 H-superfamily homologous superfamily	20 —

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7. 引用文献 (1)

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晶胞边长 a length_a	145.422 Å
晶胞边长 b length_b	170.626 Å
晶胞边长 c length_c	74.546 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	32

空间群 space_group	P 21 21 2
空间群编号 int_tables_number	18
晶系 crystal_system	—

分辨率 resolution	2.890 Å
最低分辨率 resolution_low	11.970 Å
R 因子 r_factor	0.2400
R_work r_work	0.2380
R_free r_free	0.2660

Structure of CK2 beta subunit crystallized in the presence of a p21WAF1 peptide

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合体信息 (7)

Entity 1 polymer

聚合体 Polymer

来源 Source

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 non-polymer

Entity 6 non-polymer

Entity 7 water

6. 折叠分类 (SCOP + CATH) 24 domains

SCOP 2.08 (8 domains)

CATH v4.4 (16 domains)

7. 引用文献 (1)

8. 文件与曲线 (0)