1sc5
Sigma-28(FliA)/FlgM complex
SAXS 散射曲线 SAXS Profile
P(r) 距离分布 P(r) Distribution
1. 结构基本信息 1. Structure Basics
| 条目编号 entry_id | 1sc5 |
| 沉积日期 deposition_date | 2004-02-11 |
| 结构标题 title | Sigma-28(FliA)/FlgM complex |
| 关键词 keywords | RNA POLYMERASE SIGMA FACTOR, ANTI-SIGMA FACTOR, TRANSCRIPTION, FLAGELLAR GENE REGULATION; TRANSCRIPTION |
| 实验方法 method | X-RAY DIFFRACTION |
2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)
| 回转半径 Rg (Guinier) rg_guinier | 21.80 Å |
| 回转半径 Rg (电子) rg_electron | 20.74 Å |
| 零角强度 I(0) i0 | 15472900.00 |
| 分子量 molecular_weight | 29948.0 kDa |
| 排除体积 excluded_volume | 37672 ų |
| 包络体积 envelope_volume | 46438 ų |
| 水化壳体积 shell_volume | 19477 ų |
| 包络直径 envelope_diameter | 78.3 Å |
| 壳层 Rg shell_rg | 26.36 Å |
| 包络 Rg envelope_rg | 20.86 Å |
| 形状 Rg shape_rg | 20.73 Å |
| 总 Rg total_rg | 21.59 Å |
| 总原子数 total_atoms | 2122 |
| 残基数 n_residues | 300 |
| 球谐函数阶数 n_harmonics | 20 |
| q 范围 q_range | — – 0.5000 Å−1 |
| 数据点数 n_points | 101 |
| 壳层类型 shell_type | directional |
| 溶剂电子密度 solvent_density | 0.3340 e/ų |
| 壳层衬度 contrast_shell | 0.0300 e/ų |
| CRYSOL 版本 crysol_version | 4.1.3 |
3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)
| 最大尺寸 Dmax dmax | 75.8 Å |
| Rg (实空间) rg_real | 21.79 Å |
| Rg 误差 (实空间) rg_real_error | 0.59 Å |
| I(0) (实空间) i0_real | 1.5470e+07 |
| I(0) 误差 (实空间) i0_real_error | 2.1230e+05 |
| Rg (倒空间) rg_reciprocal | 21.79 Å |
| I(0) (倒空间) i0_reciprocal | 15470000.0000 |
| 解质量估计 total_estimate | 0.7792 |
| 解质量评级 solution_quality | GOOD a GOOD solution |
| P(r) 峰数 n_peaks | 2 |
| 主峰位置 r_peak_primary | 67.9 Å |
| 偏度 Skewness skewness | 0.358 |
| 峰度 Kurtosis kurtosis | -0.185 |
| 角度范围 angular_range | — – 0.3650 Å−1 |
| 当前正则化参数 α current_alpha | 0.0000 |
| 最高正则化参数 α highest_alpha | 4512000.0000 |
| 实空间数据点数 n_real_points | 68 |
| GNOM 版本 gnom_version | 4.1.3 |
| 质量判据 quality_criteria | AN1: 0.000; Oscil: 0.732; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.934; Smooth: 0.000 |
4. 晶体学与实验 4. Crystallography & Experiment
5. 实体与聚合体信息 (2)
6. 折叠分类 (SCOP + CATH) 8 domains
SCOP 2.08 (4 domains)
| 结构域编号 domain_id | d1sc5a1 |
| 类 Class class | a — All alpha proteins |
| 折叠类型 Fold fold | a.4 — DNA/RNA-binding 3-helical bundle |
| 超家族 Superfamily superfamily | a.4.13 — Sigma3 and sigma4 domains of RNA polymerase sigma factors |
| 家族 Family family | a.4.13.1 — Sigma3 domain |
| 结构域编号 domain_id | d1sc5a2 |
| 类 Class class | a — All alpha proteins |
| 折叠类型 Fold fold | a.4 — DNA/RNA-binding 3-helical bundle |
| 超家族 Superfamily superfamily | a.4.13 — Sigma3 and sigma4 domains of RNA polymerase sigma factors |
| 家族 Family family | a.4.13.2 — Sigma4 domain |
| 结构域编号 domain_id | d1sc5a3 |
| 类 Class class | a — All alpha proteins |
| 折叠类型 Fold fold | a.177 — Sigma2 domain of RNA polymerase sigma factors |
| 超家族 Superfamily superfamily | a.177.1 — Sigma2 domain of RNA polymerase sigma factors |
| 家族 Family family | a.177.1.1 — Sigma2 domain of RNA polymerase sigma factors |
| 结构域编号 domain_id | d1sc5b_ |
| 类 Class class | a — All alpha proteins |
| 折叠类型 Fold fold | a.137 — Non-globular all-alpha subunits of globular proteins |
| 超家族 Superfamily superfamily | a.137.11 — Anti-sigma factor FlgM |
| 家族 Family family | a.137.11.1 — Anti-sigma factor FlgM |
CATH v4.4 (4 domains)
| 结构域编号 domain_id | 1sc5A01 |
| 类 Class class | 1 — Mainly Alpha |
| 架构 Architecture architecture | 10 — Orthogonal Bundle |
| 拓扑 Topology topology | 1740 — Rna Polymerase Sigma Factor; Chain: A |
| 同源超家族 H-superfamily homologous superfamily | 10 — RNA polymerase sigma factor, region 2, helix turn helix motif |
| 结构域编号 domain_id | 1sc5A02 |
| 类 Class class | 1 — Mainly Alpha |
| 架构 Architecture architecture | 20 — Up-down Bundle |
| 拓扑 Topology topology | 140 — Butyryl-CoA Dehydrogenase, subunit A; domain 3 |
| 同源超家族 H-superfamily homologous superfamily | 160 — PhyR, sigma-like (SL) domain |
| 结构域编号 domain_id | 1sc5B01 |
| 类 Class class | 6 — Special |
| 架构 Architecture architecture | 10 — Helix non-globular |
| 拓扑 Topology topology | 140 — Helix Hairpins |
| 同源超家族 H-superfamily homologous superfamily | 150 — |
| 结构域编号 domain_id | 1sc5B02 |
| 类 Class class | 6 — Special |
| 架构 Architecture architecture | 10 — Helix non-globular |
| 拓扑 Topology topology | 140 — Helix Hairpins |
| 同源超家族 H-superfamily homologous superfamily | 30 — Anti-sigma-28 factor FlgM |