1tw2

;Crystal structure of Carminomycin-4-O-methyltransferase (DnrK) in complex with S-adenosyl-L-homocystein (SAH) and 4-methoxy-e-rhodomycin T (M-ET) ;

Method: X-RAY DIFFRACTION Dmax: 102.8 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 1tw2

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1tw2
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1tw2
沉积日期 deposition_date2004-06-30
结构标题 title;Crystal structure of Carminomycin-4-O-methyltransferase (DnrK) in complex with S-adenosyl-L-homocystein (SAH) and 4-methoxy-e-rhodomycin T (M-ET) ;
关键词 keywords;Anthracycline, methyltransferase, methylate, Streptomyces, tailoring enzyme, polyketide, S-adenosyl-L-homocystein, Structural Proteomics in Europe, SPINE, Structural Genomics, TRANSFERASE ;; TRANSFERASE
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier29.83
回转半径 Rg (电子) rg_electron29.16
零角强度 I(0) i094692800.00
分子量 molecular_weight77141.0 kDa
排除体积 excluded_volume96651 ų
包络体积 envelope_volume115620 ų
水化壳体积 shell_volume34084 ų
包络直径 envelope_diameter108.4
壳层 Rg shell_rg35.16
包络 Rg envelope_rg29.45
形状 Rg shape_rg29.16
总 Rg total_rg29.69
总原子数 total_atoms5447
残基数 n_residues688
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax102.8
Rg (实空间) rg_real30.00
Rg 误差 (实空间) rg_real_error1.02
I(0) (实空间) i0_real9.4690e+07
I(0) 误差 (实空间) i0_real_error1.4430e+06
Rg (倒空间) rg_reciprocal29.93
I(0) (倒空间) i0_reciprocal94690000.0000
解质量估计 total_estimate0.8449
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary31.0
偏度 Skewness skewness0.563
峰度 Kurtosis kurtosis-0.084
角度范围 angular_range— – 0.2650 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha45790000.0000
实空间数据点数 n_real_points54
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.726; Stabil: 0.997; Sysdev: 1.000; Positv: 1.000; Valcen: 0.911; Smooth: 0.899

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (4)

6. 折叠分类 (SCOP + CATH) 10 domains

SCOP 2.08 (4 domains)

结构域编号 domain_idd1tw2a1
类 Class classa — All alpha proteins
折叠类型 Fold folda.4 — DNA/RNA-binding 3-helical bundle
超家族 Superfamily superfamilya.4.5 — 'Winged helix' DNA-binding domain
家族 Family familya.4.5.29 — Plant O-methyltransferase, N-terminal domain
结构域编号 domain_idd1tw2a2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.66 — S-adenosyl-L-methionine-dependent methyltransferases
超家族 Superfamily superfamilyc.66.1 — S-adenosyl-L-methionine-dependent methyltransferases
家族 Family familyc.66.1.12 — Plant O-methyltransferase, C-terminal domain
结构域编号 domain_idd1tw2b1
类 Class classa — All alpha proteins
折叠类型 Fold folda.4 — DNA/RNA-binding 3-helical bundle
超家族 Superfamily superfamilya.4.5 — 'Winged helix' DNA-binding domain
家族 Family familya.4.5.29 — Plant O-methyltransferase, N-terminal domain
结构域编号 domain_idd1tw2b2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.66 — S-adenosyl-L-methionine-dependent methyltransferases
超家族 Superfamily superfamilyc.66.1 — S-adenosyl-L-methionine-dependent methyltransferases
家族 Family familyc.66.1.12 — Plant O-methyltransferase, C-terminal domain

CATH v4.4 (6 domains)

结构域编号 domain_id1tw2A01
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
结构域编号 domain_id1tw2A02
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology287 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily1350
结构域编号 domain_id1tw2A03
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily150 — Vaccinia Virus protein VP39
结构域编号 domain_id1tw2B01
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
结构域编号 domain_id1tw2B02
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology287 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily1350
结构域编号 domain_id1tw2B03
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily150 — Vaccinia Virus protein VP39

7. 引用文献 (1)

8. 文件与曲线 (10)