1u0t

Crystal structure of Mycobacterium tuberculosis NAD kinase

Method: X-RAY DIFFRACTION Dmax: 94.3 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 1u0t

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1u0t
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1u0t
沉积日期 deposition_date2004-07-14
结构标题 titleCrystal structure of Mycobacterium tuberculosis NAD kinase
关键词 keywords;alpha-beta; beta sandwich, Structural Genomics, PSI, Protein Structure Initiative, TB Structural Genomics Consortium, TBSGC, SIGNALING PROTEIN, TRANSFERASE ;; SIGNALING PROTEIN, TRANSFERASE
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier27.41
回转半径 Rg (电子) rg_electron26.44
零角强度 I(0) i055472500.00
分子量 molecular_weight58185.0 kDa
排除体积 excluded_volume73116 ų
包络体积 envelope_volume90900 ų
水化壳体积 shell_volume29323 ų
包络直径 envelope_diameter97.2
壳层 Rg shell_rg33.12
包络 Rg envelope_rg26.71
形状 Rg shape_rg26.37
总 Rg total_rg27.40
总原子数 total_atoms4087
残基数 n_residues542
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax94.3
Rg (实空间) rg_real27.41
Rg 误差 (实空间) rg_real_error0.76
I(0) (实空间) i0_real5.5470e+07
I(0) 误差 (实空间) i0_real_error7.9220e+05
Rg (倒空间) rg_reciprocal27.42
I(0) (倒空间) i0_reciprocal55470000.0000
解质量估计 total_estimate0.8711
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary30.9
偏度 Skewness skewness0.350
峰度 Kurtosis kurtosis-0.261
角度范围 angular_range— – 0.2900 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha41880000.0000
实空间数据点数 n_real_points59
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.805; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.970; Smooth: 0.935

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (2)

6. 折叠分类 (SCOP + CATH) 6 domains

SCOP 2.08 (2 domains)

结构域编号 domain_idd1u0ta_
类 Class classe — Multi-domain proteins (alpha and beta)
折叠类型 Fold folde.52 — NAD kinase/diacylglycerol kinase-like
超家族 Superfamily superfamilye.52.1 — NAD kinase/diacylglycerol kinase-like
家族 Family familye.52.1.1 — NAD kinase-like
结构域编号 domain_idd1u0tb_
类 Class classe — Multi-domain proteins (alpha and beta)
折叠类型 Fold folde.52 — NAD kinase/diacylglycerol kinase-like
超家族 Superfamily superfamilye.52.1 — NAD kinase/diacylglycerol kinase-like
家族 Family familye.52.1.1 — NAD kinase-like

CATH v4.4 (4 domains)

结构域编号 domain_id1u0tA01
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily10330 — Probable inorganic polyphosphate/atp-NAD kinase; domain 1
结构域编号 domain_id1u0tA02
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology200 — Tumour Suppressor Smad4
同源超家族 H-superfamily homologous superfamily30 — Probable inorganic polyphosphate/atp-NAD kinase; domain 2
结构域编号 domain_id1u0tB01
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily10330 — Probable inorganic polyphosphate/atp-NAD kinase; domain 1
结构域编号 domain_id1u0tB02
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology200 — Tumour Suppressor Smad4
同源超家族 H-superfamily homologous superfamily30 — Probable inorganic polyphosphate/atp-NAD kinase; domain 2

7. 引用文献 (2)

8. 文件与曲线 (10)