1vf6
2.1 Angstrom crystal structure of the PALS-1-L27N and PATJ L27 heterodimer complex
SAXS 散射曲线 SAXS Profile
P(r) 距离分布 P(r) Distribution
1. 结构基本信息 1. Structure Basics
| 条目编号 entry_id | 1vf6 |
| 沉积日期 deposition_date | 2004-04-09 |
| 结构标题 title | 2.1 Angstrom crystal structure of the PALS-1-L27N and PATJ L27 heterodimer complex |
| 关键词 keywords | ;L27 domain, heterodimer, four-helical bundle, coiled-coil, hydrophobic packing interactions, PROTEIN BINDING-PROTEIN TRANSPORT COMPLEX ;; PROTEIN BINDING/PROTEIN TRANSPORT |
| 实验方法 method | X-RAY DIFFRACTION |
2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)
| 回转半径 Rg (Guinier) rg_guinier | 27.56 Å |
| 回转半径 Rg (电子) rg_electron | 27.37 Å |
| 零角强度 I(0) i0 | 10695800.00 |
| 分子量 molecular_weight | 25183.0 kDa |
| 排除体积 excluded_volume | 31773 ų |
| 包络体积 envelope_volume | 40869 ų |
| 水化壳体积 shell_volume | 14642 ų |
| 包络直径 envelope_diameter | 94.8 Å |
| 壳层 Rg shell_rg | 30.09 Å |
| 包络 Rg envelope_rg | 26.89 Å |
| 形状 Rg shape_rg | 27.33 Å |
| 总 Rg total_rg | 27.85 Å |
| 总原子数 total_atoms | 1773 |
| 残基数 n_residues | 217 |
| 球谐函数阶数 n_harmonics | 20 |
| q 范围 q_range | — – 0.5000 Å−1 |
| 数据点数 n_points | 101 |
| 壳层类型 shell_type | directional |
| 溶剂电子密度 solvent_density | 0.3340 e/ų |
| 壳层衬度 contrast_shell | 0.0300 e/ų |
| CRYSOL 版本 crysol_version | 4.1.3 |
3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)
| 最大尺寸 Dmax dmax | 95.0 Å |
| Rg (实空间) rg_real | 28.11 Å |
| Rg 误差 (实空间) rg_real_error | 1.11 Å |
| I(0) (实空间) i0_real | 1.0700e+07 |
| I(0) 误差 (实空间) i0_real_error | 1.6050e+05 |
| Rg (倒空间) rg_reciprocal | 27.95 Å |
| I(0) (倒空间) i0_reciprocal | 10690000.0000 |
| 解质量估计 total_estimate | 0.7208 |
| 解质量评级 solution_quality | REASONABLE a REASONABLE solution |
| P(r) 峰数 n_peaks | 2 |
| 主峰位置 r_peak_primary | 19.6 Å |
| 偏度 Skewness skewness | 0.553 |
| 峰度 Kurtosis kurtosis | -0.591 |
| 角度范围 angular_range | — – 0.2900 Å−1 |
| 当前正则化参数 α current_alpha | 0.0000 |
| 最高正则化参数 α highest_alpha | 1441000.0000 |
| 实空间数据点数 n_real_points | 59 |
| GNOM 版本 gnom_version | 4.1.3 |
| 质量判据 quality_criteria | AN1: 0.000; Oscil: 0.434; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.188; Smooth: 0.881 |
4. 晶体学与实验 4. Crystallography & Experiment
5. 实体与聚合体信息 (3)
6. 折叠分类 (SCOP + CATH) 9 domains
SCOP 2.08 (5 domains)
| 结构域编号 domain_id | d1vf6a_ |
| 类 Class class | a — All alpha proteins |
| 折叠类型 Fold fold | a.194 — L27 domain |
| 超家族 Superfamily superfamily | a.194.1 — L27 domain |
| 家族 Family family | a.194.1.1 — L27 domain |
| 结构域编号 domain_id | d1vf6b_ |
| 类 Class class | a — All alpha proteins |
| 折叠类型 Fold fold | a.194 — L27 domain |
| 超家族 Superfamily superfamily | a.194.1 — L27 domain |
| 家族 Family family | a.194.1.1 — L27 domain |
| 结构域编号 domain_id | d1vf6c1 |
| 类 Class class | a — All alpha proteins |
| 折叠类型 Fold fold | a.194 — L27 domain |
| 超家族 Superfamily superfamily | a.194.1 — L27 domain |
| 家族 Family family | a.194.1.1 — L27 domain |
| 结构域编号 domain_id | d1vf6c2 |
| 类 Class class | l — Artifacts |
| 折叠类型 Fold fold | l.1 — Tags |
| 超家族 Superfamily superfamily | l.1.1 — Tags |
| 家族 Family family | l.1.1.1 — Tags |
| 结构域编号 domain_id | d1vf6d_ |
| 类 Class class | a — All alpha proteins |
| 折叠类型 Fold fold | a.194 — L27 domain |
| 超家族 Superfamily superfamily | a.194.1 — L27 domain |
| 家族 Family family | a.194.1.1 — L27 domain |
CATH v4.4 (4 domains)
| 结构域编号 domain_id | 1vf6A00 |
| 类 Class class | 1 — Mainly Alpha |
| 架构 Architecture architecture | 10 — Orthogonal Bundle |
| 拓扑 Topology topology | 287 — Helix Hairpins |
| 同源超家族 H-superfamily homologous superfamily | 650 — L27 domain |
| 结构域编号 domain_id | 1vf6B00 |
| 类 Class class | 1 — Mainly Alpha |
| 架构 Architecture architecture | 10 — Orthogonal Bundle |
| 拓扑 Topology topology | 287 — Helix Hairpins |
| 同源超家族 H-superfamily homologous superfamily | 650 — L27 domain |
| 结构域编号 domain_id | 1vf6C00 |
| 类 Class class | 1 — Mainly Alpha |
| 架构 Architecture architecture | 10 — Orthogonal Bundle |
| 拓扑 Topology topology | 287 — Helix Hairpins |
| 同源超家族 H-superfamily homologous superfamily | 650 — L27 domain |
| 结构域编号 domain_id | 1vf6D00 |
| 类 Class class | 1 — Mainly Alpha |
| 架构 Architecture architecture | 10 — Orthogonal Bundle |
| 拓扑 Topology topology | 287 — Helix Hairpins |
| 同源超家族 H-superfamily homologous superfamily | 650 — L27 domain |