SAXSdb — 1w5f

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	1w5f
沉积日期 deposition_date	2004-08-06
结构标题 title	FtsZ, T7 mutated, domain swapped (T. maritima)
关键词 keywords	;CELL DIVISION, CELL-DIVISION PROTEIN, GTP-BINDING, MULTIGENE FAMILY, SEPTATION, TUBULIN, FTSZ, FILAMENT, Z-RING, GTPASE, DOMAIN SWAPPED ;; CELL DIVISION
实验方法 method	X-RAY DIFFRACTION

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	31.95 Å
回转半径 Rg (电子) rg_electron	31.64 Å
零角强度 I(0) i0	77447800.00
分子量 molecular_weight	69712.0 kDa
排除体积 excluded_volume	87453 Å³
包络体积 envelope_volume	108180 Å³
水化壳体积 shell_volume	30692 Å³
包络直径 envelope_diameter	110.3 Å
壳层 Rg shell_rg	36.09 Å
包络 Rg envelope_rg	31.67 Å
形状 Rg shape_rg	31.62 Å
总 Rg total_rg	32.07 Å
总原子数 total_atoms	4890
残基数 n_residues	632
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	107.1 Å
Rg (实空间) rg_real	32.30 Å
Rg 误差 (实空间) rg_real_error	1.09 Å
I(0) (实空间) i0_real	7.7450e+07
I(0) 误差 (实空间) i0_real_error	1.2000e+06
Rg (倒空间) rg_reciprocal	32.16 Å
I(0) (倒空间) i0_reciprocal	77440000.0000
解质量估计 total_estimate	0.8212
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	27.8 Å
偏度 Skewness skewness	0.512
峰度 Kurtosis kurtosis	-0.526
角度范围 angular_range	— – 0.2500 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	16420000.0000
实空间数据点数 n_real_points	51
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.683; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.789; Smooth: 0.833

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (4)

Entity 1 polymer

描述 description	CELL DIVISION PROTEIN FTSZ
分子量 formula_weight	38673.4 kDa
突变 mutation	YES
来源方法 src_method	man
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B
原子数 atom_count	5256

MGFDLDVEKKKENRNIPQANNLKIKVIGVGGAGNNAINRMIEIGIHGVEFVAVNTDLQVLEASNADVKIQIGENITRGLGAGGRPEIGEQAALESEEKIREVLQDTHMVFITAGFGGGTGTGASPVIAKIAKEMGILTVAIVTTPFYFEGPERLKKAIEGLKKLRKHVDTLIKISNNKLMEELPRDVKIKDAFLKADETLHQGVKGISELITKRGYIRLTSRFARIESVMKDAGAAILGIGVGKGEHRAREAAKKAMESKLIEHPVENASSIVFNITAPSNIRMEEVHEAAMIIRQNSSEDADVKFGLIFDDEVPDDEIRVIFIATRFPDEDKILFPEGDIPAIYRYGLEGLL

来源 Source

来源生物 organism	THERMOTOGA MARITIMA
NCBI 分类编号 ncbi_taxonomy_id	2336
宿主生物 host_organism	ESCHERICHIA COLI BL21(DE3)

Entity 2 non-polymer

描述 description	PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER
分子量 formula_weight	521.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

Entity 3 non-polymer

描述 description	MAGNESIUM ION
分子量 formula_weight	24.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

Entity 4 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	366

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6. 折叠分类 (SCOP + CATH) 8 domains

SCOP 2.08 (4 domains)

结构域编号 domain_id	d1w5fa1
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.32 — Tubulin nucleotide-binding domain-like
超家族 Superfamily superfamily	c.32.1 — Tubulin nucleotide-binding domain-like
家族 Family family	c.32.1.1 — Tubulin, GTPase domain

结构域编号 domain_id	d1w5fa2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.79 — Bacillus chorismate mutase-like
超家族 Superfamily superfamily	d.79.2 — Tubulin C-terminal domain-like
家族 Family family	d.79.2.1 — Tubulin, C-terminal domain

结构域编号 domain_id	d1w5fb1
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.32 — Tubulin nucleotide-binding domain-like
超家族 Superfamily superfamily	c.32.1 — Tubulin nucleotide-binding domain-like
家族 Family family	c.32.1.1 — Tubulin, GTPase domain

结构域编号 domain_id	d1w5fb2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.79 — Bacillus chorismate mutase-like
超家族 Superfamily superfamily	d.79.2 — Tubulin C-terminal domain-like
家族 Family family	d.79.2.1 — Tubulin, C-terminal domain

CATH v4.4 (4 domains)

结构域编号 domain_id	1w5fA01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	1440 — Tubulin/FtsZ, GTPase domain

结构域编号 domain_id	1w5fA02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	1330 — 60s Ribosomal Protein L30; Chain: A;
同源超家族 H-superfamily homologous superfamily	20 — Tubulin/FtsZ, C-terminal domain

结构域编号 domain_id	1w5fB01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	1440 — Tubulin/FtsZ, GTPase domain

结构域编号 domain_id	1w5fB02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	1330 — 60s Ribosomal Protein L30; Chain: A;
同源超家族 H-superfamily homologous superfamily	20 — Tubulin/FtsZ, C-terminal domain

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7. 引用文献 (1)

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晶胞边长 a length_a	181.510 Å
晶胞边长 b length_b	60.970 Å
晶胞边长 c length_c	66.790 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	8

空间群 space_group	P 21 21 2
空间群编号 int_tables_number	18
晶系 crystal_system	—

分辨率 resolution	2.000 Å
最低分辨率 resolution_low	50.000 Å
R 因子 r_factor	0.2032
R_work r_work	0.2032
R_free r_free	0.2340

fit	/app/data/saxs_computation/w5/1w5f/1w5f.fit
pddf	/app/data/pr_computation/w5/1w5f/1w5f.pr.out
profile	/app/data/saxs_computation/w5/1w5f/1w5f.dat

FtsZ, T7 mutated, domain swapped (T. maritima)

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction