2a7k

carboxymethylproline synthase (CarB) from pectobacterium carotovora, apo enzyme

Method: X-RAY DIFFRACTION Dmax: 139.8 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

SAXS profile for 2a7k

P(r) 距离分布 P(r) Distribution

P(r) distribution for 2a7k
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id2a7k
沉积日期 deposition_date2005-07-05
结构标题 titlecarboxymethylproline synthase (CarB) from pectobacterium carotovora, apo enzyme
关键词 keywordscarbapenem, carbapenam, crotonase, antibiotic, beta-lactam, Biosynthetic Protein; BIOSYNTHETIC PROTEIN
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier44.61
回转半径 Rg (电子) rg_electron44.12
零角强度 I(0) i0772715000.00
分子量 molecular_weight226090.0 kDa
排除体积 excluded_volume281360 ų
包络体积 envelope_volume356550 ų
水化壳体积 shell_volume68344 ų
包络直径 envelope_diameter135.4
壳层 Rg shell_rg47.86
包络 Rg envelope_rg43.57
形状 Rg shape_rg44.13
总 Rg total_rg44.26
总原子数 total_atoms15898
残基数 n_residues2074
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax139.8
Rg (实空间) rg_real44.47
Rg 误差 (实空间) rg_real_error1.23
I(0) (实空间) i0_real7.7270e+08
I(0) 误差 (实空间) i0_real_error1.3820e+07
Rg (倒空间) rg_reciprocal44.61
I(0) (倒空间) i0_reciprocal772800000.0000
解质量估计 total_estimate0.9068
解质量评级 solution_quality EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks1
主峰位置 r_peak_primary51.9
偏度 Skewness skewness0.111
峰度 Kurtosis kurtosis-0.787
角度范围 angular_range— – 0.1750 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha82180000.0000
实空间数据点数 n_real_points36
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.955; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.998; Smooth: 0.920

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (2)

6. 折叠分类 (SCOP + CATH) 27 domains

SCOP 2.08 (9 domains)

结构域编号 domain_idd2a7ka1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.14 — ClpP/crotonase
超家族 Superfamily superfamilyc.14.1 — ClpP/crotonase
家族 Family familyc.14.1.3 — Crotonase-like
结构域编号 domain_idd2a7kb_
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.14 — ClpP/crotonase
超家族 Superfamily superfamilyc.14.1 — ClpP/crotonase
家族 Family familyc.14.1.3 — Crotonase-like
结构域编号 domain_idd2a7kc_
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.14 — ClpP/crotonase
超家族 Superfamily superfamilyc.14.1 — ClpP/crotonase
家族 Family familyc.14.1.3 — Crotonase-like
结构域编号 domain_idd2a7kd_
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.14 — ClpP/crotonase
超家族 Superfamily superfamilyc.14.1 — ClpP/crotonase
家族 Family familyc.14.1.3 — Crotonase-like
结构域编号 domain_idd2a7ke_
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.14 — ClpP/crotonase
超家族 Superfamily superfamilyc.14.1 — ClpP/crotonase
家族 Family familyc.14.1.3 — Crotonase-like
结构域编号 domain_idd2a7kf_
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.14 — ClpP/crotonase
超家族 Superfamily superfamilyc.14.1 — ClpP/crotonase
家族 Family familyc.14.1.3 — Crotonase-like
结构域编号 domain_idd2a7kg_
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.14 — ClpP/crotonase
超家族 Superfamily superfamilyc.14.1 — ClpP/crotonase
家族 Family familyc.14.1.3 — Crotonase-like
结构域编号 domain_idd2a7kh_
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.14 — ClpP/crotonase
超家族 Superfamily superfamilyc.14.1 — ClpP/crotonase
家族 Family familyc.14.1.3 — Crotonase-like
结构域编号 domain_idd2a7ki_
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.14 — ClpP/crotonase
超家族 Superfamily superfamilyc.14.1 — ClpP/crotonase
家族 Family familyc.14.1.3 — Crotonase-like

CATH v4.4 (18 domains)

结构域编号 domain_id2a7kA01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology226 — 2-enoyl-CoA Hydratase; Chain A, domain 1
同源超家族 H-superfamily homologous superfamily10 — 2-enoyl-CoA Hydratase; Chain A, domain 1
结构域编号 domain_id2a7kA02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily1610
结构域编号 domain_id2a7kB01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology226 — 2-enoyl-CoA Hydratase; Chain A, domain 1
同源超家族 H-superfamily homologous superfamily10 — 2-enoyl-CoA Hydratase; Chain A, domain 1
结构域编号 domain_id2a7kB02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily1610
结构域编号 domain_id2a7kC01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology226 — 2-enoyl-CoA Hydratase; Chain A, domain 1
同源超家族 H-superfamily homologous superfamily10 — 2-enoyl-CoA Hydratase; Chain A, domain 1
结构域编号 domain_id2a7kC02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily1610
结构域编号 domain_id2a7kD01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology226 — 2-enoyl-CoA Hydratase; Chain A, domain 1
同源超家族 H-superfamily homologous superfamily10 — 2-enoyl-CoA Hydratase; Chain A, domain 1
结构域编号 domain_id2a7kD02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily1610
结构域编号 domain_id2a7kE01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology226 — 2-enoyl-CoA Hydratase; Chain A, domain 1
同源超家族 H-superfamily homologous superfamily10 — 2-enoyl-CoA Hydratase; Chain A, domain 1
结构域编号 domain_id2a7kE02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily1610
结构域编号 domain_id2a7kF01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology226 — 2-enoyl-CoA Hydratase; Chain A, domain 1
同源超家族 H-superfamily homologous superfamily10 — 2-enoyl-CoA Hydratase; Chain A, domain 1
结构域编号 domain_id2a7kF02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily1610
结构域编号 domain_id2a7kG01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology226 — 2-enoyl-CoA Hydratase; Chain A, domain 1
同源超家族 H-superfamily homologous superfamily10 — 2-enoyl-CoA Hydratase; Chain A, domain 1
结构域编号 domain_id2a7kG02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily1610
结构域编号 domain_id2a7kH01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology226 — 2-enoyl-CoA Hydratase; Chain A, domain 1
同源超家族 H-superfamily homologous superfamily10 — 2-enoyl-CoA Hydratase; Chain A, domain 1
结构域编号 domain_id2a7kH02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily1610
结构域编号 domain_id2a7kI01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology226 — 2-enoyl-CoA Hydratase; Chain A, domain 1
同源超家族 H-superfamily homologous superfamily10 — 2-enoyl-CoA Hydratase; Chain A, domain 1
结构域编号 domain_id2a7kI02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily1610

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8. 文件与曲线 (10)