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2abm

Crystal Structure of Aquaporin Z Tetramer Reveals both Open and Closed Water-conducting Channels

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Aquaporin Z

Escherichia coli

UniProt P60844

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein homooligomer Homooligomer Protein 4 2-O-octyl-beta-D-glucopyranose × 3 BIS(((3S,4S,5R,6R)-5-(ETHYL(PHOSPHORYLOXY))-3,4,6-TRIHYDROXY-TETRAHYDRO-2H-PYRAN-2-YL)METHYL) HYDROGEN PHOSPHATE × 1 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 3 PHOSPHATE ION × 2 3-PHOSPHOGLYCERIC ACID × 1 (1S)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTANOYLOXY)METHYL]ETHYL OCTANOATE × 1 water × 4 Consistent with protein count
2 Protein homooligomer Homooligomer Protein 4 2-O-octyl-beta-D-glucopyranose × 1 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 PHOSPHATE ION × 2 3-PHOSPHOGLYCERIC ACID × 1 (1S)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTANOYLOXY)METHYL]ETHYL OCTANOATE × 1 water × 4 Consistent with protein count
3 Protein homooligomer Homooligomer Protein 16 2-O-octyl-beta-D-glucopyranose × 8 BIS(((3S,4S,5R,6R)-5-(ETHYL(PHOSPHORYLOXY))-3,4,6-TRIHYDROXY-TETRAHYDRO-2H-PYRAN-2-YL)METHYL) HYDROGEN PHOSPHATE × 2 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 PHOSPHATE ION × 8 3-PHOSPHOGLYCERIC ACID × 4 (1S)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTANOYLOXY)METHYL]ETHYL OCTANOATE × 4 water × 16 Consistent with protein count
4 Protein homooligomer Homooligomer Protein 16 2-O-octyl-beta-D-glucopyranose × 8 BIS(((3S,4S,5R,6R)-5-(ETHYL(PHOSPHORYLOXY))-3,4,6-TRIHYDROXY-TETRAHYDRO-2H-PYRAN-2-YL)METHYL) HYDROGEN PHOSPHATE × 2 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 PHOSPHATE ION × 8 3-PHOSPHOGLYCERIC ACID × 4 (1S)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTANOYLOXY)METHYL]ETHYL OCTANOATE × 4 water × 16 Consistent with protein count
5 Protein homooligomer Homooligomer Protein 16 2-O-octyl-beta-D-glucopyranose × 8 BIS(((3S,4S,5R,6R)-5-(ETHYL(PHOSPHORYLOXY))-3,4,6-TRIHYDROXY-TETRAHYDRO-2H-PYRAN-2-YL)METHYL) HYDROGEN PHOSPHATE × 2 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 PHOSPHATE ION × 8 3-PHOSPHOGLYCERIC ACID × 4 (1S)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTANOYLOXY)METHYL]ETHYL OCTANOATE × 4 water × 16 Consistent with protein count
6 Protein homooligomer Homooligomer Protein 16 2-O-octyl-beta-D-glucopyranose × 8 BIS(((3S,4S,5R,6R)-5-(ETHYL(PHOSPHORYLOXY))-3,4,6-TRIHYDROXY-TETRAHYDRO-2H-PYRAN-2-YL)METHYL) HYDROGEN PHOSPHATE × 2 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 PHOSPHATE ION × 8 3-PHOSPHOGLYCERIC ACID × 4 (1S)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTANOYLOXY)METHYL]ETHYL OCTANOATE × 4 water × 16 Consistent with protein count
7 Protein homooligomer Homooligomer Protein 8 2-O-octyl-beta-D-glucopyranose × 4 BIS(((3S,4S,5R,6R)-5-(ETHYL(PHOSPHORYLOXY))-3,4,6-TRIHYDROXY-TETRAHYDRO-2H-PYRAN-2-YL)METHYL) HYDROGEN PHOSPHATE × 1 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 PHOSPHATE ION × 4 3-PHOSPHOGLYCERIC ACID × 2 (1S)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTANOYLOXY)METHYL]ETHYL OCTANOATE × 2 water × 8 Consistent with protein count
8 Protein homooligomer Homooligomer Protein 8 2-O-octyl-beta-D-glucopyranose × 4 BIS(((3S,4S,5R,6R)-5-(ETHYL(PHOSPHORYLOXY))-3,4,6-TRIHYDROXY-TETRAHYDRO-2H-PYRAN-2-YL)METHYL) HYDROGEN PHOSPHATE × 1 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 PHOSPHATE ION × 4 3-PHOSPHOGLYCERIC ACID × 2 (1S)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTANOYLOXY)METHYL]ETHYL OCTANOATE × 2 water × 8 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
1rc2 2o9d 2o9f

3 assemblies

Homodimer

None

Homotetramer
1rc2 1rc2 2abm 2abm 2o9d 2o9d 2o9e 2o9f 2o9f 2o9g 3nk5 3nk5

16 assemblies; showing the first 12

Other homooligomers
2abm 2abm 2abm 2abm 2abm 2abm

6 assemblies

Heteromeric or nucleic-acid complex
8uy6

1 assemblies

View Construct and Data Evidence
UniProt name AQPZ_ECOLI
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–231； UniProt 1–231 Author chain B； PDBConstruct 1–231； UniProt 1–231 Author chain C； PDBConstruct 1–231； UniProt 1–231 Author chain D； PDBConstruct 1–231； UniProt 1–231 Author chain E； PDBConstruct 1–231； UniProt 1–231 Author chain F； PDBConstruct 1–231； UniProt 1–231 Author chain G； PDBConstruct 1–231； UniProt 1–231 Author chain H； PDBConstruct 1–231； UniProt 1–231

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id2abm
Deposition date deposition_date2005-07-15
Structure title titleCrystal Structure of Aquaporin Z Tetramer Reveals both Open and Closed Water-conducting Channels
Keywords keywordsAQUAPORIN, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2abm__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2abm__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

2abm__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）26.87 Å
Rg (electron density)25.83 Å
Total Rg26.89 Å
Atom count6733
Residues908
Excluded volume122950 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 2abm__assembly_1__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 2abm__assembly_2__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
3 1 2abm__assembly_3__model_1 hexadecameric （16） Success 4.1.3-1-20251215 (887e7ef) View Download
4 1 2abm__assembly_4__model_1 hexadecameric （16） Success 4.1.3-1-20251215 (887e7ef) View Download
5 1 2abm__assembly_5__model_1 hexadecameric （16） Success 4.1.3-1-20251215 (887e7ef) View Download
6 1 2abm__assembly_6__model_1 hexadecameric （16） Success 4.1.3-1-20251215 (887e7ef) View Download
7 1 2abm__assembly_7__model_1 octameric （8） Success 4.1.3-1-20251215 (887e7ef) View Download
8 1 2abm__assembly_8__model_1 octameric （8） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (8)

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6. Fold Classification (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

Domain ID domain_idd2abma1
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.19 — Aquaporin-like
Superfamily Superfamily superfamilyf.19.1 — Aquaporin-like
Family Family familyf.19.1.1 — Aquaporin-like
Domain ID domain_idd2abmb1
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.19 — Aquaporin-like
Superfamily Superfamily superfamilyf.19.1 — Aquaporin-like
Family Family familyf.19.1.1 — Aquaporin-like
Domain ID domain_idd2abmc1
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.19 — Aquaporin-like
Superfamily Superfamily superfamilyf.19.1 — Aquaporin-like
Family Family familyf.19.1.1 — Aquaporin-like
Domain ID domain_idd2abmd1
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.19 — Aquaporin-like
Superfamily Superfamily superfamilyf.19.1 — Aquaporin-like
Family Family familyf.19.1.1 — Aquaporin-like
Domain ID domain_idd2abme1
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.19 — Aquaporin-like
Superfamily Superfamily superfamilyf.19.1 — Aquaporin-like
Family Family familyf.19.1.1 — Aquaporin-like
Domain ID domain_idd2abmf1
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.19 — Aquaporin-like
Superfamily Superfamily superfamilyf.19.1 — Aquaporin-like
Family Family familyf.19.1.1 — Aquaporin-like
Domain ID domain_idd2abmg1
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.19 — Aquaporin-like
Superfamily Superfamily superfamilyf.19.1 — Aquaporin-like
Family Family familyf.19.1.1 — Aquaporin-like
Domain ID domain_idd2abmh1
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.19 — Aquaporin-like
Superfamily Superfamily superfamilyf.19.1 — Aquaporin-like
Family Family familyf.19.1.1 — Aquaporin-like

CATH v4.4 (8 domains)

Domain ID domain_id2abmA00
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1080 — Glycerol uptake facilitator protein
Homologous superfamily homologous superfamily10 — Glycerol uptake facilitator protein.
Domain ID domain_id2abmB00
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1080 — Glycerol uptake facilitator protein
Homologous superfamily homologous superfamily10 — Glycerol uptake facilitator protein.
Domain ID domain_id2abmC00
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1080 — Glycerol uptake facilitator protein
Homologous superfamily homologous superfamily10 — Glycerol uptake facilitator protein.
Domain ID domain_id2abmD00
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1080 — Glycerol uptake facilitator protein
Homologous superfamily homologous superfamily10 — Glycerol uptake facilitator protein.
Domain ID domain_id2abmE00
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1080 — Glycerol uptake facilitator protein
Homologous superfamily homologous superfamily10 — Glycerol uptake facilitator protein.
Domain ID domain_id2abmF00
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1080 — Glycerol uptake facilitator protein
Homologous superfamily homologous superfamily10 — Glycerol uptake facilitator protein.
Domain ID domain_id2abmG00
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1080 — Glycerol uptake facilitator protein
Homologous superfamily homologous superfamily10 — Glycerol uptake facilitator protein.
Domain ID domain_id2abmH00
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1080 — Glycerol uptake facilitator protein
Homologous superfamily homologous superfamily10 — Glycerol uptake facilitator protein.
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7. Citations (1)