2apo

Crystal Structure of the Methanococcus jannaschii Cbf5 Nop10 Complex

Method: X-RAY DIFFRACTION Dmax: 74.3 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

SAXS profile for 2apo

P(r) 距离分布 P(r) Distribution

P(r) distribution for 2apo
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id2apo
沉积日期 deposition_date2005-08-16
结构标题 titleCrystal Structure of the Methanococcus jannaschii Cbf5 Nop10 Complex
关键词 keywords;Protein-Protein complex, Box H/ACA, snoRNP, Pseudouridine synthase, RNA modification, Zinc ribbon, PUA domain, Isomerase-RNA binding protein COMPLEX ;; Isomerase/RNA binding protein
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier24.18
回转半径 Rg (电子) rg_electron23.24
零角强度 I(0) i025909700.00
分子量 molecular_weight40066.0 kDa
排除体积 excluded_volume50554 ų
包络体积 envelope_volume60866 ų
水化壳体积 shell_volume22373 ų
包络直径 envelope_diameter76.4
壳层 Rg shell_rg29.77
包络 Rg envelope_rg23.37
形状 Rg shape_rg23.22
总 Rg total_rg24.12
总原子数 total_atoms2795
残基数 n_residues356
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax74.3
Rg (实空间) rg_real24.15
Rg 误差 (实空间) rg_real_error0.44
I(0) (实空间) i0_real2.5910e+07
I(0) 误差 (实空间) i0_real_error3.3220e+05
Rg (倒空间) rg_reciprocal24.16
I(0) (倒空间) i0_reciprocal25910000.0000
解质量估计 total_estimate0.9134
解质量评级 solution_quality EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary25.5
偏度 Skewness skewness0.234
峰度 Kurtosis kurtosis-0.645
角度范围 angular_range— – 0.3300 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha4392000.0000
实空间数据点数 n_real_points65
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.969; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.986; Smooth: 0.978

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (5)

6. 折叠分类 (SCOP + CATH) 6 domains

SCOP 2.08 (3 domains)

结构域编号 domain_idd2apoa1
类 Class classb — All beta proteins
折叠类型 Fold foldb.122 — PUA domain-like
超家族 Superfamily superfamilyb.122.1 — PUA domain-like
家族 Family familyb.122.1.1 — PUA domain
结构域编号 domain_idd2apoa2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.265 — Pseudouridine synthase
超家族 Superfamily superfamilyd.265.1 — Pseudouridine synthase
家族 Family familyd.265.1.2 — Pseudouridine synthase II TruB
结构域编号 domain_idd2apob_
类 Class classg — Small proteins
折叠类型 Fold foldg.41 — Rubredoxin-like
超家族 Superfamily superfamilyg.41.16 — Nop10-like SnoRNP
家族 Family familyg.41.16.1 — Nucleolar RNA-binding protein Nop10-like

CATH v4.4 (3 domains)

结构域编号 domain_id2apoA01
类 Class class2 — Mainly Beta
架构 Architecture architecture30 — Roll
拓扑 Topology topology130 — Archaeosine Trna-guanine Transglycosylase; Chain: A, domain 4
同源超家族 H-superfamily homologous superfamily10 — PUA domain
结构域编号 domain_id2apoA03
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily3190
结构域编号 domain_id2apoB00
类 Class class2 — Mainly Beta
架构 Architecture architecture20 — Single Sheet
拓扑 Topology topology28 — Rubrerythrin, domain 2
同源超家族 H-superfamily homologous superfamily40 — H/ACA ribonucleoprotein complex, subunit Nop10

7. 引用文献 (1)

8. 文件与曲线 (10)